• PharmaTutor
    10 April 2013

    miRNAs A NOVEL TARGET FOR ANTICANCER THERAPY

    ABOUT AUTHORS:
    Ketan M. Parmar*, Ritesh N. Sharma
    S.K.Patel College of Pharmaceutical Education & research,
    Department of Pharmaceutical chemistry, GANPAT UNIVERSITY.
    *brave_student_90@yahoo.com

    ABSTRACT
    With the development of technologies to look at the expression levels of hundreds of miRNAs at a time and the clear role of miRNAs in cancers, groups began looking at miRNAs profiles of different cancers,especially the circulating miRNAs. We intended to make sure whether circulating miRNAs could be a promising biomarker of human cancers. Method: We comprehensively searched the Cochrane Library, Medline and EMbase from 1966 to Nov 2009 for the following terms: (“miRNA” or “microRNA”) and (“tumor” or “carcinoma”) and (“plasma” or “serum” or “circulating”). Detailed information was extracted from studies that met the inclusion criteria: blood-based miRNAs in human cancers and studies published in the English literature. Results: The current review show that different researches use different measurement methods which might impact the results;Cancers treatment might have an effect on circulating miRNAs; some miRNAs are multi-faceted RNA; small sample size might produce selection bias. Furthermore, because of the lack of randomized controlled trials and the heterogeneous nature of the available data, no attempt was made to perform quantitativemeta-analyses.
    In this review, based on those researches, circulating miRNAs are promising and difficulties for their future application for diagnosing human cancers.

  • 8 April 2013
    RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF MONTELUKAST SODIUM AND DESLORATADINE IN COMBINED DOSAGE FORM

    ABOUT AUTHORS:
    Rima M. Bankar*, Dipti B. Patel
    Department of Pharmaceutical Analysis,
    Shree S. K. Patel College of Pharmaceutical Education & Research, Ganpat University,
    Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    *rima.banker@yahoo.com

    ABSTRACT
    A novel, precise, accurate and rapid isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for  simultaneous determination of Montelukast Sodium and Desloratadine. The method showed adequate separation for Montelukast Sodium and Desloratadine  and best resolution was achieved with ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size) using Acetonitrile-Methanol-Water (15:80:5, v/v) as a mobile phase at a flow rate of 1.0 ml/min and wavelength of 283 nm. The calibration curves were linear over the concentration ranges of 5-50 μg/ml for Montelukast Sodium and Desloratadine. The limit of detection (LOD) and limit of quantification (LOQ) for Montelukast Sodium were 0.33 and 1.01 μg/ml while for Desloratadine were 0.10 and 0.31 μg/ml, respectively. All the analytes were separated in less than 6.0 min. The proposed method could be applied for routinelaboratory analysis of Montelukast Sodium and Desloratadine in pharmaceutical dosage form. Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in synthetic mixture of both drugs with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.

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  • 7 April 2013
    Requirement as Store Keeper, Assistant Pharmacist, B.Pharm Assistant, Lab Technitian Government Ayurvedic hospitals/colleges/pharmacies - 11 posts

    Applications invited in Government Ayurvedic hospitals/colleges/pharmacies for paramedical staff for five years fixed renumeration by Director, Indian medicine and Homeopathy of system, Block no. 1, 2nd floor, Dr. Jivraj Mehta Bhavan, Gandhinagar, Gujarat.

    Posts: Store Keeper, Assistant Pharmacist, B.Pharm Assistant, Lab Technitian

  • 7 April 2013
    Career as Quality Control Officer in Reputed API Bulk Drug Company - B.Pharm/M.Sc/B.Sc may apply

    Leading for pharma company.

    Post: Quality Control Officer

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  • 7 April 2013
    Require Staff Nurse in Hospitals, India’s leading super specialty hospital chain is renowned for Cardiac care.

    Hospitals, India’s leading super specialty hospital chain is renowned for Cardiac care.

    Post: Staff Nurse

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  • 5 April 2013
    Opportunity to work as Pharmacist in Assam Rifles - 23 posts

    Assam Rifles recruitment rally  will be held at Kohima(Nagaland), NRS-Dimapur and Shillong(Meghalaya), {NRS-Guwahati} from24 Jun 2013 onwards for enrolment to 431 vacancies (male candidates) from following States for the posts mentioned against each :-

    POST: PHARMACIST - Total 23 posts

  • 4 April 2013
    SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM BY RATIO SPECTRA DERIVATIVE SPECTROPHOTOMETRY METHOD IN SYNTHETIC MIXTURE

    ABOUT AUTHORS:
    Rinku B Patel*1, Paresh U Patel2, Bharat G Patel3, Anil C Patel2
    1Department of Pharmaceutical Analysis, Centre For Health Science Studies, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    2Department of Quality Assurance, Centre For Health Science Studies, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    3Aspee college of Home Science and Nutrition, S.D.Agricultural University, S.K.Nagar-385506, Banaskantha, Gujarat, India.
    *rinkupatel5890@gmail.com

    ABSTRACT
    Simple, accurate, precise, and sensitive ratio spectra derivative spectrophotometric method for simultaneous estimation of Eperisone hydrochloride (EPE) and Diclofenac sodium(DIC) in synthetic mixture have been developed and validated. The ratio derivative spectroscopic method involves measurement of first derivative amplitude of ratio spectra at 247 nm for EPE and 218.4 nm for DIC as two wavelengths for estimation. Beer's law is obeyed in the concentration range of 2-18 μg/ml for both EPE and DIC. LOD values for EPE and DIC are found to be 0.0634 μg/ml and 0.5386 μg/ml, respectively. LOQ values for EPE and DIC are found to be 0.1921 μg/ml and 1.6321 μg/ml, respectively. The method was validated statistically and recovery studies were carried out. It was found to be accurate, precise and reproducible. The method was applied to the assay of the drugs in synthetic mixture, which were found in the range of 98.0% to 102.0% of the labeled value for both Eperisone hydrochloride and Diclofenac sodium. Hence, the method herein described can be successfully applied in quality control of synthetic mixture.

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  • 4 April 2013
    Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ibuprofen and Chlorzoxazone in Synthetic Mixture

    ABOUT AUTHORS
    Anil C. Patel*, Dr Paresh U. Patel , Rinku B. Patel
    Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research,
    Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    *anilpatel002@gmail.com

    ABSTRACT
    A novel, precise, accurate and rapid isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for  simultaneous determination of Ibuprofen and Chlorzoxazone. The method showed adequate separation for Ibuprofen and Chlorzoxazone and best resolution was achieved with ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size) using Acetonitrile-Phosphate buffer pH 3.5 - Methenol (20:20:60, v/v; pH adjusted to 3.5 with O-phosphoric acid and TEA(Tetra ethyl amine) as a mobile phase at a flow rate of 0.7 ml/min and wavelength of 221 nm. The calibration curves were linear over the concentration ranges of 2-30 μg/ml for Ibuprofen and Chlorzoxazone. The limit of detection (LOD) and limit of quantification (LOQ) for Ibuprofen were 0.96 and 2.92 μg/ml while for Chlorzoxazone were 0.69 and 2.09 μg/ml, respectively. All the analytes were separated in less than 6.0 min. The proposed method could be applied for routinelaboratory analysis of Ibuprofen and Chlorzoxazone in pharmaceutical dosage form. Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in synthetic mixture of both drugs with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.

  • 3 April 2013
    Pharmaceutical product pricing policies in India: A simplified view on latest amendments

    When the domestic pharmacy market is forecasted to touch US$20 billion by 2015, it was essential to do amendments in previous laws directly affecting pharmaceutical market in India. Amendment regulations are drafted by parliamentary counsel’s office for the purpose of excellence in regulation and only published in gazettes. Major change experienced by Indian pharmaceutical market recently is in pharmaceutical pricing policies.

  • 25 March 2013
    Opening as Regional Manager – Institutional Sales,Business Manager in Unichem Labs

    Unichem Laboratories has grown to become one of India’s most respected pharmaceutical companies. It is committed to delivering better health through superior products. By combining strategic research and in-depth industry knowledge, Unichem aims to transform itself into a global pharmaceutical drug company with an increasing focus on cutting-edge research and developed markets.

     

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