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  • SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION OF DICLOFENAC SODIUM AND EPERISONE HYDROCHLORIDE USING ABSORBANCE RATIO METHOD IN CAPSULE DOSAGE FORM

    ABOUT AUTHORS:
    Lalit F. Raiyani*1, Dharanant V. Borakhatariya2, Bhargav D. Patel3, Kuldip R. Marwada4, Dr.Priti D. Trivedi5, Mr.Rajendra K. Patel6
    1Parul institute of Pharmacy, Vadodara
    2B. K. Modi Government Pharmacy College, Rajkot
    3Ramanbhai Patel College of Pharmacy, Changa
    4R. K. College of Pharmacy, Rajkot
    5Professor at K. B. Institute of Pharmaceutical Education and Research, Gandhinagar
    6Lecturer at K. B. Institute of Pharmaceutical Education and Research, Gandhinagar
    *lalitraiyani@gmail.com

    ABSTRACT:
    A simple, rapid, sensitive, precise and accurate UV-spectrophotometric method (absorbance ratio) was developed and validated for simultaneous estimation of Diclofenac sodium and Eperisone hydrochloride in pharmaceutical capsule dosage form. In absorbance ratio method absorbance measurement of sample at 239.2 nm (isoabsorbtive point, λ1) and 256 nm, λ2.The absorbance ratio method was developed using methanol as solvent. Developed method is linear between 4-12µg/ml and 5-15µg/ml for diclofenac sodium and eperisone hydrochloride respectively. The mean % recovery was found to be 99.68% & 99.14% for diclofenac sodium and eperisone hydrochloride respectively.

  • METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND INDAPAMIDE BY DIFFERENT SPECTROPHOTOMETRIC AND RP-HPLC METHODS IN BULK DRUG AND PHARMACEUTICAL FORMULATION

    ABOUT AUTHORS:
    Madhuri tadiparthi
    Chalapathi institute of pharmaceutical sciences,
    guntur, a.p, india.
    tadiparthimadhuri@gmail.com

    ABSTRACT:
    Amlodipine
     (as besylate, mesylate or maleate) is a long-acting calcium channel blocker (dihydropyridine (DHP) class) used as an anti-hypertensive and in the treatment of angina. Indapamide is a thiazide diuretic used in the treatment of hypertension, as well as decompensated cardiac failure. Six new, simple, accurate and precise methods have been developed and validated according to ICH guidelines for the simultaneous estimation of Amlodipine and Indapamide in their combined dosage form (four UV-Spectrophotometric, one colorimetric and one RP-HPLC methods).
    First method is based on simultaneous estimation using two wavelengths, 365 nm (λmaxof AMLO) and 279 nm (λmaxof INDA) by simultaneous equation method. The second method involves the use of first order derivative technique, here 293 nm, the zero crossing point of AMLO, 279 nm, the zero crossing point of INDA were used for the estimation. The third method is based on Q-absorption Ratio method using two wavelengths 365 nm (λmaxof AMLO) and 312 nm (Isoabsorptive point). In the dual wavelength method two wave lengths 270 nm and 288 nm were selected as λ1 and λ2 for the estimation of AMLO, INDA shows the same absorbance at these wavelengths. Similarly, wavelengths 350 nm and 378 nm were selected as λ1 and λ2 for the estimation of INDA, AMLO shows the same absorbance at these wavelengths.Colorimetry:  The method is based on use of MBTH reagent for simultaneous estimation of AMLO and INDA using two wavelengths, 626 nm (λmaxof AMLO) and 600 nm (λmaxof INDA).

  • RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF MONTELUKAST SODIUM AND DESLORATADINE IN COMBINED DOSAGE FORM

    ABOUT AUTHORS:
    Rima M. Bankar*, Dipti B. Patel
    Department of Pharmaceutical Analysis,
    Shree S. K. Patel College of Pharmaceutical Education & Research, Ganpat University,
    Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    *rima.banker@yahoo.com

    ABSTRACT
    A novel, precise, accurate and rapid isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for  simultaneous determination of Montelukast Sodium and Desloratadine. The method showed adequate separation for Montelukast Sodium and Desloratadine  and best resolution was achieved with ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size) using Acetonitrile-Methanol-Water (15:80:5, v/v) as a mobile phase at a flow rate of 1.0 ml/min and wavelength of 283 nm. The calibration curves were linear over the concentration ranges of 5-50 μg/ml for Montelukast Sodium and Desloratadine. The limit of detection (LOD) and limit of quantification (LOQ) for Montelukast Sodium were 0.33 and 1.01 μg/ml while for Desloratadine were 0.10 and 0.31 μg/ml, respectively. All the analytes were separated in less than 6.0 min. The proposed method could be applied for routinelaboratory analysis of Montelukast Sodium and Desloratadine in pharmaceutical dosage form. Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in synthetic mixture of both drugs with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.

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  • SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM BY RATIO SPECTRA DERIVATIVE SPECTROPHOTOMETRY METHOD IN SYNTHETIC MIXTURE

    ABOUT AUTHORS:
    Rinku B Patel*1, Paresh U Patel2, Bharat G Patel3, Anil C Patel2
    1Department of Pharmaceutical Analysis, Centre For Health Science Studies, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    2Department of Quality Assurance, Centre For Health Science Studies, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    3Aspee college of Home Science and Nutrition, S.D.Agricultural University, S.K.Nagar-385506, Banaskantha, Gujarat, India.

    *rinkupatel5890@gmail.com

    ABSTRACT
    Simple, accurate, precise, and sensitive ratio spectra derivative spectrophotometric method for simultaneous estimation of Eperisone hydrochloride (EPE) and Diclofenac sodium(DIC) in synthetic mixture have been developed and validated. The ratio derivative spectroscopic method involves measurement of first derivative amplitude of ratio spectra at 247 nm for EPE and 218.4 nm for DIC as two wavelengths for estimation. Beer's law is obeyed in the concentration range of 2-18 μg/ml for both EPE and DIC. LOD values for EPE and DIC are found to be 0.0634 μg/ml and 0.5386 μg/ml, respectively. LOQ values for EPE and DIC are found to be 0.1921 μg/ml and 1.6321 μg/ml, respectively. The method was validated statistically and recovery studies were carried out. It was found to be accurate, precise and reproducible. The method was applied to the assay of the drugs in synthetic mixture, which were found in the range of 98.0% to 102.0% of the labeled value for both Eperisone hydrochloride and Diclofenac sodium. Hence, the method herein described can be successfully applied in quality control of synthetic mixture.

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  • Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ibuprofen and Chlorzoxazone in Synthetic Mixture

    ABOUT AUTHORS
    Anil C. Patel*, Dr Paresh U. Patel , Rinku B. Patel
    Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research,
    Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
    *anilpatel002@gmail.com

    ABSTRACT
    A novel, precise, accurate and rapid isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for  simultaneous determination of Ibuprofen and Chlorzoxazone. The method showed adequate separation for Ibuprofen and Chlorzoxazone and best resolution was achieved with ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size) using Acetonitrile-Phosphate buffer pH 3.5 - Methenol (20:20:60, v/v; pH adjusted to 3.5 with O-phosphoric acid and TEA(Tetra ethyl amine) as a mobile phase at a flow rate of 0.7 ml/min and wavelength of 221 nm. The calibration curves were linear over the concentration ranges of 2-30 μg/ml for Ibuprofen and Chlorzoxazone. The limit of detection (LOD) and limit of quantification (LOQ) for Ibuprofen were 0.96 and 2.92 μg/ml while for Chlorzoxazone were 0.69 and 2.09 μg/ml, respectively. All the analytes were separated in less than 6.0 min. The proposed method could be applied for routinelaboratory analysis of Ibuprofen and Chlorzoxazone in pharmaceutical dosage form. Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in synthetic mixture of both drugs with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.

  • AN UPDATE ON FUNDAMENTALS OF UV- VISIBLE SPECTROSCOPY

    ABOUT AUTHORS:
    Prashanta Kr. Deb1*, Kaushik Nath Bhoumik2,
    1Dept. of Pharmacy, Tripura University; Suryamaninagar –799022, Agartala, Tripura (W), India.
    2Regional Institute of Pharmaceutical Science & Technology, Agartala–799005, Tripura (W), India.
    *shaandeb2010@gmail.com

    INTRODUCTION
    Spectroscopy is the measurement and interpretation of Electromagnetic radiation (EMR) absorbed or emitted when the molecules or atoms or ions of a sample move from one energy state to another energy state. This change may be ground state to exited state or vice-versa. EMR is made up of discrete particles called photons. EMR has got both wave characteristic as well as particle characteristics. This means that it can travel in vacuum also.

  • A REVIEW ON MÖSSBAUER SPECTROSCOPY

    About Authors
    Deepak Kumar Shukla*, Nazia Shahid, Vikas Kumar Alaria
    Rajasthan Pharmacy College, Bhankrota, Jaipur (Raj.)
    *deepakshukla.pharma@gmail.com

    Abstract
    Over the past several years spectroscopy has become the preeminent technique for determining the structure of organic compounds. The study of recoilless nuclear resonant absorption or fluorescence is more commonly known as Mossbauer spectroscopy. From its first origins in 1957, it has grown rapidly to become one of the most important research methods in solid-state physics and chemistry. Mossbauer spectroscopy uses the nuclear properties to get information regarding the environment surrounding the nucleus. This technique is now valid application in diverse fields, such as solid state physics, metallurgy, chemistry and biochemistry. For example, it is possible to use this method for estimating the iron or tin content in ores, alloys and wasters in a non-destructive manner to concentration down to 0.03 percent in a short time of  the order of 10 minutes. The technique can also detect the relative percentage of  different charged states of the same atom, for example fe2+ and Fe3+ present in the material. This is somewhat difficult to get from any other technique.

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  • DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF TOLPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN SYNTHETIC MIXTURE

    ABOUT AUTHORS
    Satish A. Patel, Kaushik P Hariyani*
    Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research,

  • DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CHLORZOXAZONE AND DICLOFENAC SODIUM IN COMBINATION

    ABOUT AUTHORS:
    Satish A. Patel, Kalpesh M. Prajapati*
    Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research,
    Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.

  • A REVIEW ON PHARMACEUTICAL ANALYSIS OF MASS SPECTROSCOPY

    ABOUT AUTHORS:
    G.Venkateswarlu*, M.Muthukumaran, B.krishnamoorthy, Ameren nishat
    Montessori Siva Sivani Institute of Science & Technology College of Pharmacy-Mylavaram,
    Vijayawada, Andhra pradesh-521230
    *venkateswarlu460@gmail.com

    ABSTRACT
    The goal of this review is to provide a guide for understanding current MS technology. Mass spectrometry (MS) has progressed to become a powerful analytical tool for both quantitative and qualitative applications. Proteomics research, in particular, increasingly depends on MS technologies. Basically, any information gathered from a mass spectrometer comes from the analysis of gas-phase ions. There are three main components of a mass spectrometer: an ionization source, a mass analyzer and a detector the name ‘mass spectrometry’ is a misnomer of sorts. The mass is not what is measured; instead, mass spectrometry determines the mass-to-charge (m/z) ratio or a property related to m/z .A mass spectrum is a plot of ion abundance versus m/z, although in many cases the x-axis is labelled ‘mass’ rather than m/z. The spectrum is presented in terms of Daltons (Da) per unit charge The ability of mass spectrometry analyzing proteins and other biological extracts is due to the advances gained through the development of soft ionization techniques such as electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) that can transform biomolecules into ions. Regardless of the ionization source, the sensitivity of a mass spectrometer is related to the mass analyzer where ion separation occurs.  

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