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  • DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR ASSAY OF AZITHROMYCIN IN POWDER FOR ORAL SUSPENSION

    About Author:
    Swapna.G*
    Department of pharmaceutical Pharmaceutical & Quality Assurance,
    Nirmala College of Pharmacy, Mangalagiri, Atmakuru, Guntur -522 203.
    *swapna.goday.gs@gmail.com

    Abstract
    A  simple,  precise  and  accurate  reversed phase liquid chromatographic method has been  developed  for  the  assay  of  azithromycin  in  powder  for oral suspension. The chromatographic separation was achieved on a Asahipak ODP 40 E(250 mm × 4.6 mm, 5 μm)  analytical  column.  A  mixture  of  methanol–ammonium dihydrogen phosphate (0.05M)  (30:70, v/v)  (pH 9.0)  was  used  as  the  mobile  phase,  at   a  flow  rate  of 1.5 mLmin-1  and  detector  wavelength  at  210 nm. The retention time of  azithromycin was  found to be at  8.0 min. The validation of the proposed method was carried out for specificity, linearity, accuracy, precision, robustness and stability indicating assay. The linear  dynamic range is from   382–1208 μgmL-1 for  azithromycin. The percentage recovery  obtained  for  azithromycin is 101.0%.  The developed method can be used for pharmaceutical dosage form and  in process testing.

  • PRELIMINARY CHARACTERIZATION OF ROCK SALT IN PRESENCE OF CITRIC ACID BY FTIR SPECTROSCOPY

    ABOUT AUTHOR:
    BINDU.S
    ANNAMACHRYA COLLEGE OF PHARMACY,
    RAJAMPET,KADAPA Dist, A.P, INDIA.
    sirasalabindupharmacy@gmail.com

    INTRODUCTION
    Rock salt, also known as halite is the naturally occurring crystalline salt. It is the mineral form of sodium chloride or the table salt that we use. However, rock salt does not look like the regular table salt. Instead, it naturally forms in large isometric crystals. Moreover, rock salt can be white like table salt or it can be red, orange, yellow or blue in color. The color of rock salt basically depends on the amount, and the kind of impurities present in it. Along with sodium, rock salt contains many other minerals including, calcium, zinc, iron, magnesium, potassium, copper and several other trace minerals. Like table salt, rock salt too has great many uses. rock salt in massive bags for the purpose of keeping down ice on the roads in the winter.

  • SPECTROPHOTOMETRIC ESTIMATION OF TOLPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN SYNTHETIC MIXTURE BY DUAL WAVELENGTH METHOD

    ABOUT AUTHORS:
    Hiral H. Patel*, Paresh U. Patel,
    Department of Pharmaceutical Analysis, Center for Health and Science Studies,
    Ganpat University, Ganpat Vidyanagar – 384012,
    Mehsana, Gujarat, India.
    *patel.hiral2210@gmail.com

    ABSTRACT
    This method describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic methodfor the simultaneous determination of tolperisone hydrochloride (TOL) and diclofenac sodium (DIC) in bulk and synthetic mixture. In this study, dual wavelength spectroscopic method was used for simultaneous determination of tolperisone hydrochloride and diclofenac sodium using the absorbance difference at two wavelengths. The absorbance differences of 257 nm and 306.20 nm were selected for the estimation of TOL and the absorbance differences of 243.40 nm and 265.40 nm were selected for estimation of DIC. The method was found to be linear (r2>0.999) in the range of 2- 18 μg/ml for tolperisone hydrochloride. The linear correlation was obtained (r2>0.997) in the range of 2- 18 μg/ml for diclofenac sodium. The limit of determination was 0.66 and 0.27 μg/ml for tolperisone hydrochloride and diclofenac sodium respectively. The limit of quantification was 2.00 and 0.83 μg/ml. The method was successfully applied for simultaneous determination of tolperisone hydrochloride and diclofenac sodium in binary mixture.

  • A REVIEW ON ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PHARMACEUTICAL TECHNOLOGY

    ABOUT AUTHORS:
    Rajani Pathuri*, M.Muthukumaran, B.Krishnamoorthy, Amreen Nishat
    Montessori Siva Sivani Institute of Science & Technology-College of pharmacy
    Mylavaram, Vijayawada, Andhrapradesh-521230
    *rajani.prakash4@gmail.com

    ABSTRACT
    Analytical methods development and validation play important roles in the discovery, development and Manufacture of pharmaceuticals. Method development is the process of proving that an analytical method is acceptable for use to measure the concentration of an API in a specific compounded dosage form which allow simplified procedures to be employed to verify that an analysis procedure, accurately and consistently will deliver a reliable measurement of an active ingredient in a compounded preparation. The analytical method validation is essential for analytical method development and tested extensively for specificity, linearity, accuracy, precision, range, detection limit, quantization limit, and robustness. In summary, analytical method development and validation allows to confirm that an accurate and reliable potency measurement of a pharmaceutical preparation can be performed. 

  • A CRITICAL REVIEW ON CALIBRATION OF ANALYTICAL INSTRUMENTS

    About Authors:
    Manoranjan Thunuguntla*, B.Krishnamoorthy, M.Muthukumaran, Amreen Nishat, Vasu Naik
    Montessori Siva Sivani Institute Of Science & Technology College Of Pharmacy,
    Mylavaram, Krishna District,
    Andhra Pradesh

    * manozph@gmail.com

    Abstract1:
    Analytical instruments are used for specific analysis of drugs and pharmaceuticals. So, regular performance verification is made to ensure that instrument used in the analytical purpose should be properly calibrated and validated “to demonstrate that it is suitable for its intended purpose”. This article is prepared in the sense to get all the information on calibration of basic analytical instruments in laboratory and industrial scale of scientific institutions. The scope of this article is to get the procedures of calibration of analytical instruments at here. Calibration of instruments with their procedure and precautions of Thermometer, Ph Meter, Karl Fisher, Polarimeter, Conductivity Meter, Tablet Fraibilator, Hardness Tester, Disintegration Test Apparatus, Dissolution Test Apparatus, Potentiometer and U. V. Spectrophotometer are mentioned below.

  • SIMULTANEOUS ESTIMATION OF GLIMEPIRIDE, PIOGLITAZONE HYDROCHLORIDE AND METFORMIN HYDROCHLORIDE BY DERIVATIVE SPECTROPHOTOMETRY METHOD

    About Authors:
    Wagh Vinod. T.
    Dept. of Pharmaceutics,
    DCS’s. A.R.A. College of Pharmacy, Nagaon,
    Dhule-424005 MH. India
    wagh.vinod517@gmail.com

  • EVALUATION OF QUALITY OF FRESH SOYA-BEAN OIL AVAILABLE IN BANGLADESH

    ABOUT AUTHORS:
    MR. Zubair Khalid labu*1, Md. Abdul Bake2, Razia Sultana3
    1,3
    Department of Pharmacy, World University of Bangladesh, Dhaka 1205, Bangladesh,
    2
    Department of Pharmacy, Gono University, Savar, Dhaka 1344, Bangladesh.
    * zubairlabu@yahoo.com

    ABSTRACT
    Hydrogenated soya-bean oil is the product obtained by refining, bleaching, hydrogenation and deodorisation of oil obtained from seeds ofGlycine soja Sieb. and Zucc. andGlycine max (L.) Merr. (G. hispida (Moench) Maxim.). Soya-bean oilis a vegetable oil extracted from the seeds of the soybean. It is plant based and less harmful saturated fats and offers nutrition and health related benefits.  The main objective of this work has been done for the evaluation and analyzing the quality of soya-bean oil available in local market. For the examination of soyabean oil six fresh samples of different sources were collected randomly from the market. British pharmacopoeia (BP) specifications were used for the determination of acid, peroxide and iodine value. The results were compared with the standard specified by Bangladesh standard and testing institute (BSTI). After three months study we observed that physical examination of all the samples were complies with the specification and chemical examination such as, In case of peroxide value, all the samples were found to comply with the specification and only two (S-5 and S-6) of them have comparatively higher value but remain within the limit. But In case of iodine value, five samples were within the limit, and only one sample (S-6) was out of specification. After over third month analysis we observed that all the sample value were higher than from first month study. The consumers which have to take this spurious product must be careful to take this because it may be harmful if adulterated.

  • ESTIMATION OF DRONEDARONE HYDROCHLORIDE BY SPECTROPHOTOMETRIC METHOD IN PHARMACEUTICAL FORMULATION

    About Authors:
    Kashyap K. Bhatt, Emanual Michael Patelia*, Ishani Amin
    Department of Pharmaceutical Chemistry and Analysis,
    Indukaka Ipcowala College of Pharmacy,
    New Vallabh Vidyanagar – 388121, Gujarat, India.
    *ricky.emanual@gmail.com

  • DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD OF DRONEDARONE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL FORMULATION

    About Authors:
    Gedam V. K., Shrivastav S. R., Raut N.A.
    Department of Pharmaceutical Sciences RTM, Nagpur University, Amravati Road,
    Nagpur- 440033.
    *vkgedam8@hotmail.com

    ABSTRACT
    The present research work discusses the development of a UV spectrophotometric method for Dronedarone hydrochloride. Simple, accurate, cost efficient and reproducible spectrophotometric method has been developed for the estimation of Dronedarone hydrochloride in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement at maximum wavelength (λmax) at 290nm using methanol as a solvent. The percentage recovery of Dronedarone hydrochloride ranged from 98.48 to 98.96% in pharmaceutical dosage form. Beers law was obeyed in the concentration range of 2-30μg/ml having line equation y = 0.0397x - 0.0060 with correlation coefficient of 0.9998. Results of the analysis were validated statistically and by recovery study.

  • BIOPHARMACEUTICAL ANALYSIS NEED AND PROCESS INVOLVED IN PRELIMINARY TREATMENT OF BIOLOGICAL SAMPLES FOR USING LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROSCOPY(LC-MS/MS): A REVIEW

    About Authors:
    *S.B.Muthu Vadivel, R.Suresh Kumar1, A.Tamil Selvan2, R.Suthakaran3
    Department of Pharmaceutical Analysis and Quality Assurance
    Teegala Ram Reddy College of Pharmacy

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