Nimish Talaviya1*, Nirav Chandegara2, Rakesh Radadiya3, Chetana Ribadiya4, Chandani Joshi5, Dhara Kanjaria6
1,5Department of Q.A., Smt. R. D. Gardi B. Pharmacy College, Rajkot
2Department of Pharmacology, K. B. Institute of Technology, Gandhinagar
3Department of Q.A., Mts. V.B. Manvar College of Pharmacy, Dumiyani
4Department of Q.A., Smt. R. B. Patel Mahila Pharmacy College, Atkot
6Department of Q.A., Shree H. N. Shukla Institute of Pharmaceutical Education & Research, Rajkot
This paper presents a RP-HPLC method for the simultaneous estimation of Paracetamol and Pamabrom in its synthetic mixture. The process was carried out on C18 (250 x 4.6mm, 5µ) (Spincotech Pvt. Ltd.) column using Phosphate buffer pH 4.5: Acetonitrile (75:25 %v/v) where pH was maintained using 0.1% orthophosphoric acid as a mobile phase at a flow rate of 0.8ml/min. Detection wavelength was fixed at isobestic point 268 nm. Linearity was observed in the concentration range of 10-50 μg/ml for Paracetamol (r2=0.9986) and 15-75 μg/ml for Pamabrom (r2=0.9971). The retention time of Paracetamol and Pamabrom was found to be 2.665 and 6.907 minutes respectively with resolution 13.70. The developed method is simple, precise, specific, robust, rapid, reproducible, and sensitive and it can be used for estimation ofParacetamol and Pamabrom in its synthetic mixture.