Pharmaceutical Analysis Articles

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TOLPERISONE HYDROCHLORIDE AND PARACETAMOL IN PHARMACEUTICAL FORMULATION

About Author:
Monali G. Patel*, Ragin R. Shah, Krupa H. Shah, Bushra M. Malik
Department of Pharmacy, Pharmaceutical Quality Assurance Laboratory,
Arihant School of Pharmacy & BRI, Adalaj,
Gandhinagar-382421, Gujarat, India.
*monali_patel317@yahoo.in

ABSTRACT
A simple, novel, sensitive, precise and specific validated RP-HPLC method was developed for simultaneous determination of Tolperisone Hydrochloride (TOL) and Paracetamol (PCM) in Pharmaceutical Formulation (Tablet). The chromatographic separation was achieved on C18 Licrosphere column as a stationary phase usingMethanol: Tetrahydrofuran (90: 10 v/v, pH 8.2 with 0.1% v/v TEA) as mobile phase at detection wavelength 254 nm. The linearity range was 3-27 µg/ml for TOL and 10-90 µg/ml for PCM with Rt of 3.593 min and 5.023 min for PCM and TOL respectively. The correlation coefficient (r) values were found to be found to be 0.9994 and 0.9998 for TOL and PCM, respectively.Precision study showed % CV values less than 2% for both TOL and PCM, respectively in all selected concentrations. The % Recoveries of TOL and PCM are in the range of 99.96- 100.71 % and 99.73- 100.49 %, respectively. The assay results of TOL and PCM are 100.05 % and 99.98 %, respectively. This is comparable to labeled claim. The method was validated as per the International Conference on Harmonization (ICH) guidelines.The proposed validated method was successfully used for the quantitative analysis of commercially available dosage form.

A CRITICAL REVIEW ON PHARMACEUTICAL ANALYSIS IN LIQUID CHROMATOGRAPHY - MASS SPECTROSCOPY (LCMS)

About Authors:
P. Udaya Lakshmi*, B.Krishnamoorthy, M.Muthukumaran, Amreen Nishat
Montessori Siva Sivani Institute of Science&Technology College of Pharmacy-Mylavaram,
Vijayawada, Andhrapradesh-521230
*udayalakshmi53@gmail.com

ABSTRACT
Liquid chromatography (LC) combined with mass spectrometry (MS) is a powerful tool for qualitative and quantitative analytics of organic molecules from various matrices, and the use of this hyphenated technique is very common in bioanalytical laboratories. LC-MS is an analytical technique that couples high resolution chromatographic separation with sensitive and specific mass spectrometric detection. The technique is still fast developing, particularly in the mass spectrometry area, with vastly improved sensitivity and resolution. It is probably the most powerful technique currently available for pharmaceutical analysis. The role of LC/MS in the pharmaceutical industry during the past decade is examined, and key elements for recent success are illustrated to include significant advances in instrumentation, methodology, and application. The applications are highlighted with reference to the analysis opportunity and analysis strategy implemented. The applications of LC-MS to the studies of in vitro and in vivo drug metabolism, identification and characterization of impurities in pharmaceuticals, analysis of chiral impurities in drug substances and high-throughput LC-MS-MS systems for applications in the "accelerated drug discovery" process are described.

Automated analysis

About Authors:
Patelia emanual michael*, Keyur B Ahir
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India.
*ricky.emanual@gmail.com

Abstract:
Ethane, cyclohexane, SO2, ethylene and other gaseous components. Liquid solutions also can be monitored by the analyzers. Near IR measurements using tungsten filaments lamps as sources are often used to monitor water concentrations. IR reflectance can be used to monitor water concentration in some solids, such as in paper. If moisture is present some of the incident radiation is observed and less is reflected at the wavelengths characteristics of water.

DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD OF DRONEDARONE HYDROCHLORIDE IN BULK AND PHARMACEUTICAL FORMULATION

About Authors:
Gedam V. K., Shrivastav S. R., Raut N.A.
Department of Pharmaceutical Sciences RTM, Nagpur University, Amravati Road,
Nagpur- 440033.
*vkgedam8@hotmail.com

ABSTRACT
The present research work discusses the development of a UV spectrophotometric method for Dronedarone hydrochloride. Simple, accurate, cost efficient and reproducible spectrophotometric method has been developed for the estimation of Dronedarone hydrochloride in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement at maximum wavelength (λmax) at 290nm using methanol as a solvent. The percentage recovery of Dronedarone hydrochloride ranged from 98.48 to 98.96% in pharmaceutical dosage form. Beers law was obeyed in the concentration range of 2-30μg/ml having line equation y = 0.0397x - 0.0060 with correlation coefficient of 0.9998. Results of the analysis were validated statistically and by recovery study.

BIOPHARMACEUTICAL ANALYSIS NEED AND PROCESS INVOLVED IN PRELIMINARY TREATMENT OF BIOLOGICAL SAMPLES FOR USING LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROSCOPY(LC-MS/MS): A REVIEW

About Authors:
*S.B.Muthu Vadivel, R.Suresh Kumar1, A.Tamil Selvan2, R.Suthakaran3
Department of Pharmaceutical Analysis and Quality Assurance
Teegala Ram Reddy College of Pharmacy
Saroor nagar, Meerpet, Hyderabad – 97.
*muthuvadivelanalyst@gmail.com

Abstract
Swift growth in the use of LC-MS/MS for the analysis of drugs in biological matrices has been compelled by the need for timely and high-quality data at many stages in drug discovery and development process: from high throughput screening of drug candidates and rapid data generation for pre-clinical studies to almost real-time analysis of clinical samples. Prompt and rational method development, validation, and transfer play a pivotal role in achieving the goals of faster, better, and cheaper for pharmacokinetic studies since this could easily account for more than 50% of the time and labor resources for a moderate-sized project. In this review article, Processing and treatment of biological samples for LC-MS/MS methods will be critically reviewed and discussed. Nature of biological samples, preliminary treatment, extraction procedures, and how chromatography coupled with mass spectroscopy just a few topics covered in this review. Other interesting approaches for improving the storage and stability of biological sample extracts as well as multiplexing of LC columns will also are discussed.

A REVIEW ON INSTRUMENTATION OF THERMAL ANALYSIS METHOD: DTA, DSC

About Authors:
Bhupender Kumar*, Assit. Prof. Prasant Beniwal, Monish Sharma, Ramchandra
Seth G.L. Bihani S.D. College of Technical Education
(Institute Of Pharmaceutical Sciences And Drug Research),
Sri Ganganagar, Rajasthan
*bhupendra.nimiwal@gmail.com

Abstract:
Thermoanalytical methods essentially techniques that are based entirely on the concept of heating a sample followed by well-defined modified procedures, such as : gravimetric analysis, differential thermal analysis (DTA) and differential scanning calorimetry (DSC). Thermogravimetric analysis measured weight change, differential scanning calorimetry measured heats and temperature of transitions and reactions, differential thermal analysis (DTA) measured temperatures of transitions and reactions.


“COMPARATIVE ANALYSIS OF VARIOUS PHYSICOCHEMICAL PROPERTIES OF MARKETED PARACETAMOL FORMULATION”

About Authors:
Sailesh Narayan*, Ankit Diwan
Sagar institute of Pharmacy and Technology,
Gandhi Nagar, Bhopal
*saileshcology@yahoo.co.in

INTRODUCTION
A tablet is a pharmaceutical dosage form. It comprises a mixture of active substances and excipients, usually in powder form, pressed or compacted from  a powder into a solid dose. The excipients can include diluents, binders or granulating agents, glidants and lubricants to ensure efficient tabletting; disintegrants to promote tablet break-up in the digestive tract; sweeteners or flavours to enhance taste; and pigments to make the tablets visually attractive. A polymer coating is often applied to make the tablet smoother and easier to swallow, to control the release rate of the active ingredient, to make it more resistant to the environment or to enhance the tablet's appearance. These are compressed tablets. formulated to deliver an accurate dosage to a specific site; it is usually taken orally, but can be administered sublingually, buccally, rectally or intravaginally. Medicinal tablets were originally made in the shape of a disk of whatever color their components determined, but are now made in many shapes and colors to help distinguish different medicines. Tablets are often stamped with symbols, letters, and numbers, which enable them to be identified.

AN OVERVIEW OF LIQUID CHROMATOGRAPHY COUPLED WITH TANDEM MASS SPECTROSCOPY (LC-MS/MS)

About Authors:
*S.B.Muthu Vadivel, R.Suresh Kumar, A.Tamil Selvan, R.Suthakaran
Department of Pharmaceutical Analysis and Quality Assurance
Teegala Ram Reddy College of Pharmacy
Saroor nagar, Meerpet, Hyderabad – 97.
*muthuvadivelanalyst@gmail.com

Abstract
Mass spectrometry has been applied to almost every area of research being pursued today. Studies of diverse subjects, such as cancer research, identification of drugs, forensic analysis, atmospheric end-water environmental analysis, combustion, and lasers have benefited from mass spectrometry. In some of these studies, the mass spectrometer is used both as a chemical reactor and as an analytical instrument. Because of these diverse applications, no person or group can completely review the field of mass spectroscopy. This chapter discusses instrumental designs and techniques, ionization processes, ion–molecule reactions, high-temperature systems, and sampling of reactive species. Any tandem mass spectroscopy has four basic components: (I) a system by which the sample to be studied is introduced into the instrument, (II) an ion source where ions that are characteristic of the sample are produced, (III) an analyzer region where the ion beam is sorted into its various mass-to-charge ratios, and (IV) a detector system where the separated ion beams are collected and by some method, rendered observable.

INSTRUMENTATION OF ESR SPECTROSCOPY

About Authors:
Lila dhar*1,Surender Jalandra
1Seth G. L. Bihani S. D. College Of Technical Education,
Institute Of Pharmaceutical Sciences & Drug Research,
Gaganpath, Sri Ganganagar, Rajasthan 335001
*ldbudania@gmail.com

ABSTRACT
Electron paramagnetic resonance spectroscopy (EPR) is a powerful tool for investigating paramagnetic species, including organic radicals, inorganic radicals, and triplet states. The basic principles behind EPR are very similar to the more ubiquitous nuclear magnetic resonance spectroscopy (NMR), except that EPR focuses on the interaction of an external magnetic field with the unpaired electron(s) in a molecule, rather than the nuclei of individual atoms. EPR has been used to investigate kinetics, mechanisms, and structures of paramagnetic species and along with general chemistry and physics, has applications in biochemistry, polymer science, and geosciences. The use of cavity stabilised Impatt diode oscillators for ESR spectroscopy is discussed in different experimental conditions: i.e. as microwave sources in reflection cavity homodyne spectrometers, and as marginal oscillators in which the oscillator cavity (a TE011 cylindrical cavity) is the observing cavity. The sensitivity of this second configuration has been theoretically evaluated for the case in which the Impatt itself is used as a detecting element and in which an external detector is used. For each situation the sensitivity has been measured with a DPPH sample at various power levels giving a sensitivity which is comparable with the best commercial units.

SIMULTANEOUS ESTIMATION OF TRAMADOL HCL, PARACETAMOL AND DOMPERIDONE IN PHARMACEUTICAL FORMULATION BY RP-HPLC METHOD

About Authors:
Keyur B.ahir, Emanual M. Patelia*, Falgun A.Mehta
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India.
*ricky.emanual@gmail.com

Abstract:
A simple, precise, rapid, selective, and economic reversed phase high-performance liquid chromatography (RP-HPLC) method has been established for simultaneous analysis of A Phenomenex C18 (250´4.6 mm i.d) chromatographic column equilibrated with mobile phase 0.02M Potassium dihydrogen o-phosphate/acetonitrile (55/45, v/v) adjusted to pH 6.5 with Triehtylamine (1% v/v) was used. Mobile phase flow rate was maintained at 1 ml/min and effluents were monitored at 278 nm. The sample was injected using a 20 ml fixed loop, and the total run time was 10 min. Experimental conditions such as pH of mobile phase, column saturation time, selection of wavelength, etc. were critically studied and the optimum conditions were selected. The retention time for PCM, DMP and TMD were 3.76 min, 5.18 min and 4.28 min, respectively. The calibration curve for DMP, PCM and TMD was found to be linear in the range of 0.2 - 1 mg/ml, 6.5 – 32.5 µg/ml and 0.75 – 3.75 mg /ml with a correlation coefficient of 0.9998, 0.9976 and 0.9974. The detection limits for PCM, DMP and TMD were 20 ng/ml, 1.06 ng/ml and 2 ng/ml, respectively, while quantitation limits were 60 ng/ml, 3.23 ng/ml and 6 ng/ml, respectively. This HPLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined tablet dosage forms.

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