A REVIEW ON ANALYTICAL METHODS FOR ESTIMATION OF ASPIRIN, CLOPIDOGREL BISULPHATEAND ROSUVASTATIN CALCIUMIN PHARMACEUTICAL DOSAGE FORM

Pharma courses

pharma courses



{ DOWNLOAD AS PDF }

ABOUT AUTHORS
1Jyoti Yadav , 2Amit Sharma*
1Maharishi Dayanad University Rohatak
2 NIMS University, Jaipur

*amitsharma84945@gmail.com

ABSTRACT
This review article is intended to highlight the analytical methods of aspirin, clopidogrel and rosuvastatin in individual as well as combined pharmaceutical dosage form. Aspirin, clopidogrel and rosuvastatin play an important role in the various cardiovascular diseases. Aspirin and clopidogrel are the antiplatelet whereas Rosuvastatin is antilipemic agent which are used in the treatment of various cardiovascular diseases, cerebrovascular and peripheral vascular diseases. Now these days these drugs are easily available in the market in their individual form as well as in their combined dosage form. Aspirin, clopidogrel and rosuvastatin are official in the pharmacopoeias .Various analytical methods have been reported for the estimation of these drugs in their individual form as well as in their combined dosage form.

REFERENCE ID: PHARMATUTOR-ART-2518

PharmaTutor (Print-ISSN: 2394 - 6679; e-ISSN: 2347 - 7881)

Volume 5, Issue 9

Received On: 22/04/2017; Accepted On: 14/05/2017; Published On: 01/09/2017

How to cite this article: Yadav J, Sharma A;A Review on Analytical Methods for Estimation of Aspirin, Clopidogrel Bisulphate and Rosuvastatin Calcium in Pharmaceutical Dosage Form; PharmaTutor; 2017; 5(9);35-46

Introduction to Analytical Method
There are various analytical methods are used nowthese days for the estimation .Various analytical methods like potentiometer, HPLC ,aqueous and non-aqueous titrations are used in the field of analysis .Aqueous and non-aqueous titrations are also used in the field of analysis .But now these days HPLC plays an important role in the field of analysis for the quantitative determination.
HPLC is referred as high pressure liquid chromatography which is a separation technique based on the solid stationary phase and liquid mobile phase [1].Chromatography is mass transfer process involve adsorption. The active component of the column is adsorbent which is granular material of solid particles(silica, polymers). The principle of separation in the normal phase mode and reverse phase mode is adsorption in which the substances travel /separate according to their relative affinities. Now these days HPLC plays an important role in the field of pharmaceutical analysis for the separation of various substances from the mixture of substances [2].

Introduction to Drug Profile
Aspirin
Aspirin is known as acetylsalicylic acid which is still the most commonly used NSAID to treat pain and inflammation [3].Aspirin is 2-acetyloxy benzoic which is COX inhibitor. Aspirin is white crystalline powder[4] which is freely soluble in chloroform and in ether, slightly soluble in water with having molecular formula C9H8O 4 and molecular weight 180.2g/mol.

Chemical Structure of Aspirin

Pharmacological action [5]
The analgesic, antipyretic, and anti-inflammatory effects of acetylsalicylic acid are due to actions by both the acetyl and the salicylate portions of the intact molecule as well as by the active salicylate metabolite. Acetylsalicylic acid directly and irreversibly inhibits the activity of both types of cyclooxygenase (COX-1 and COX-2) to decrease the formation of precursors of prostaglandins and thromboxane’s from arachidonic acid. This makes acetylsalicylic acid different from other NSAIDS (such as diclofenac and ibuprofen) which are reversible inhibitors.

 

Summary of Analytical Methods for Aspirin
Official Methods for Aspirin6-8

SR.

No.

Official in

METHOD

BRIEF INTRODUCTION

REF.

NO

1

IP-2010

(Aspirin tablet)

Potentiometric Titration

Titrate:-

Tablet Powder Equivalent to 0.5 gm. Aspirin in 30ml of the 0.5M Sodium Hydroxide

Titrant:-

 0.5 M HCl

 

1ml of 0.5M NaOH is Equivalent to .0.04504 gm. of Aspirin

6

2

BP-2009

Potentiometric Titration

Titrate:-

1gm Aspirin in 10ml Ethanol, Add50ml of the 0.5M Sodium Hydroxide

Titrant:-

 0.5 M HCl

 

1ml of 0.5M NaOH is Equivalent to .0.04504 gm. of Aspirin

7

3

USP30-NF25

(Aspirin Tablet)

Liquid Chromatography

Mobile phase:-

Water (pH 3.4): Acetonitrile (85:15)

Column:-

Packing L1,  (300 mm × 4.0 mm)

Flow rate:-

 2 ml/min

Wavelength:-

 285 nm.

8

Reported Methods for Aspirin14-26

SR.

No.

DRUGS

METHOD

BRIEF INTRODUCTION

REF.

NO

1

Aspirin

RP-HPLC

Mobile phase:-

Sodium Perchlorate Buffer, pH (2.5): Acetonitrile: Isopropyl alcohol (85:14:1)

Column:-

C18, (100 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.5 ml/min

Wavelength:-

 275 nm.

9

2

Aspirin and Metoprolol

RP-HPLC

Mobile phase:-

Phosphate Buffer, (pH 4.6): Methanol (20:80)

Column:-

Phenomenex Luna C18, (250 mm × 4.6 mm, 5µ,)

Flow rate:-

 0.8 ml/min

Wavelength:-

 230 nm.

10

3

Aspirin, Ramipril,

Hydrochlorothiazide, Simvastatin And Atenolol

RP-HPLC

Mobile phase:-

Methanol: Water (95:5)

Column:-

 Hypersil Gold C18, (250 mm × 4.6 mm, 5µ,)

Flowrate:-

 1 ml/min

Wavelength:-

 230 nm.

11

4

Aspirin and

Prasugrel

RP-HPLC

Mobile phase:-

Acetonitrile: Acetate Buffer (75:25)

Column:-

 Luna C18 (150 mm × 4.6 mm, 5µ,)

Flow rate:-

 0 .6 ml/min

Wavelength:-

 245 nm

12

5

Aspirin and

Prasugrel

Stability indicating RP-HPLC

Mobile phase:-

Acetonitrile: Methanol: Water pH (3) (30:10:60)

Column:-

 Kromasil-100 C18 (150 mm × 4.6 mm, 5µ,)

Flow rate:-

 1 ml/min

Wavelength:-

 245 nm

13

6

Aspirin and Salicylic acid

RP-HPLC

Mobile phase:-

 Acetonitrile: Trifluoroacetic acid 0.05%  (30:70)

Column:-

 Waters C18 (250 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 230 nm

14

7

Amlodipine Besylate, Atenolol

and Aspirin

RP-HPLC

Mobile phase:-

 Methanol: Phosphate Buffer (pH 7.0) (70:30)

Column:-

 BDS C18 (250 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 235 nm

15

8

Aspirin And Aspirin Derivatives

RP-HPLC

Mobile phase:-

 Acetonitrile: Water (60:40)

Column:-

 Kromasil C18 (180 mm × 4.6 mm, 5µ,)

Flowrate:-

 1.0 ml/min

Wavelength:-

 277 nm

16

9

Aspirin, Caffeine and

Orphenadrine citrate

RP-HPLC

Mobile phase:-

 Methanol: Phosphate Buffer, pH3 (65:35)

Column:-

 Acclaim C18 (250 mm × 4.6 mm, 5µ,)

Flowrate:-

 1.0 ml/min

Wavelength:-

 215 nm

17

10

Aspirin and

Dipyridamole

RP-HPLC

Mobile phase:-

 0.1 % Phosphoric acid: Acetonitrile (75:25)

Column:-

 RP C18 (50 mm × 4.6 mm, 3.5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 227 nm

18

11

Aspirin and Esomeprazole

Magnesium

RP-HPLC

Mobile phase:-

 Acetonitrile: Methanol: Phosphate Buffer, pH 3.0 (25:25:50)

Column:-

 ODS BP C18 (200 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 230 nm

19

12

Ramipril, Aspirin and Simvastatin

RP-HPLC

Mobile phase:-

 Acetonitrile: Methanol: 0.5% phosphoric acid (10:70:20)

Column:-

 ODS BP C18 (200 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 226 nm

20

13

Aspirin,

Salicylic Acid, and Caffeine

RP-HPLC

Mobile phase:-

 Water: Methanol: Acetic acid (69:28:3)

Column:-

 Hypersil C18 (150 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 275 nm

21

Clopidogrel Bisulphate[22-23]
Clopidogrel Bisulphate is an antiplatelet agent which is used to inhibit the aggregation of platelets which inhibits the blood clots  .The drug is Methyl (+)-(S)-α-(2-chlorophenyl)-6,7dihydrothieno [3,2-c] pyridine-5(4H) acetate sulfate. Clopidogrel bisulphate is insoluble in water.Clopidogrel Bisulphate is an irreversible inhibitor of P2Y12. The molecular formula of clopidogrel bisulphate is C16H16ClNO2S.H2SO4 and the molecular mass is 419.03 g/mol.

Pharmacological Action [23]
Clopidogrel is an anti-platelet agent which acts by direct inhibition of ADP. The anti –aggregating activity of the clopidogrel bisulphate is due to the biotransformation of the drug to 2-oxo-clopidogrel by enzyme P450-1A.Clopidogrel Bisulphate is mostlyused in the myocardial infarction, stroke and peripheral artery disease

Summary of Analytical Methods for Clopidogrel Bisulphate
Official Methods for Clopidogrel Bisulphate[24-25]

SR. NO

OFFICIAL IN

METHOD

DESCRIPTION

REF. NO

  1

IP 2010

(Clopidogrel Tablet)

Chiral Chromatography

Mobile phase:-

Phosphate Buffer: Acetonitrile (75:25)

Column:-

 Chiral Recognition Protein (15 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  220 nm

24

2

USP30-NF25

( Clopidogrel Tablet)

Chiral Chromatography

Mobile phase:-

Phosphate Buffer: Acetonitrile (75:25)

Column:-

 Packing L57 (15 cm X 4.6 mm)

Flow Rate:-

1.0 ml/min

Wavelength:-

  220 nm

25

NOW YOU CAN ALSO PUBLISH YOUR ARTICLE ONLINE.

SUBMIT YOUR ARTICLE/PROJECT AT editor-in-chief@pharmatutor.org

Subscribe to Pharmatutor Alerts by Email

FIND OUT MORE ARTICLES AT OUR DATABASE


 

Pages

FIND MORE ARTICLES