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A REVIEW ON ANALYTICAL METHODS FOR ESTIMATION OF ASPIRIN, CLOPIDOGREL BISULPHATEAND ROSUVASTATIN CALCIUMIN PHARMACEUTICAL DOSAGE FORM

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ABOUT AUTHORS
1Jyoti Yadav , 2Amit Sharma*
1Maharishi Dayanad University Rohatak
2 NIMS University, Jaipur

*amitsharma84945@gmail.com

ABSTRACT
This review article is intended to highlight the analytical methods of aspirin, clopidogrel and rosuvastatin in individual as well as combined pharmaceutical dosage form. Aspirin, clopidogrel and rosuvastatin play an important role in the various cardiovascular diseases. Aspirin and clopidogrel are the antiplatelet whereas Rosuvastatin is antilipemic agent which are used in the treatment of various cardiovascular diseases, cerebrovascular and peripheral vascular diseases. Now these days these drugs are easily available in the market in their individual form as well as in their combined dosage form. Aspirin, clopidogrel and rosuvastatin are official in the pharmacopoeias .Various analytical methods have been reported for the estimation of these drugs in their individual form as well as in their combined dosage form.

REFERENCE ID: PHARMATUTOR-ART-2518

PharmaTutor (Print-ISSN: 2394 - 6679; e-ISSN: 2347 - 7881)

Volume 5, Issue 9

Received On: 22/04/2017; Accepted On: 14/05/2017; Published On: 01/09/2017

How to cite this article: Yadav J, Sharma A;A Review on Analytical Methods for Estimation of Aspirin, Clopidogrel Bisulphate and Rosuvastatin Calcium in Pharmaceutical Dosage Form; PharmaTutor; 2017; 5(9);35-46

Introduction to Analytical Method
There are various analytical methods are used nowthese days for the estimation .Various analytical methods like potentiometer, HPLC ,aqueous and non-aqueous titrations are used in the field of analysis .Aqueous and non-aqueous titrations are also used in the field of analysis .But now these days HPLC plays an important role in the field of analysis for the quantitative determination.
HPLC is referred as high pressure liquid chromatography which is a separation technique based on the solid stationary phase and liquid mobile phase [1].Chromatography is mass transfer process involve adsorption. The active component of the column is adsorbent which is granular material of solid particles(silica, polymers). The principle of separation in the normal phase mode and reverse phase mode is adsorption in which the substances travel /separate according to their relative affinities. Now these days HPLC plays an important role in the field of pharmaceutical analysis for the separation of various substances from the mixture of substances [2].

Introduction to Drug Profile
Aspirin
Aspirin is known as acetylsalicylic acid which is still the most commonly used NSAID to treat pain and inflammation [3].Aspirin is 2-acetyloxy benzoic which is COX inhibitor. Aspirin is white crystalline powder[4] which is freely soluble in chloroform and in ether, slightly soluble in water with having molecular formula C9H8O 4 and molecular weight 180.2g/mol.

Chemical Structure of Aspirin

Pharmacological action [5]
The analgesic, antipyretic, and anti-inflammatory effects of acetylsalicylic acid are due to actions by both the acetyl and the salicylate portions of the intact molecule as well as by the active salicylate metabolite. Acetylsalicylic acid directly and irreversibly inhibits the activity of both types of cyclooxygenase (COX-1 and COX-2) to decrease the formation of precursors of prostaglandins and thromboxane’s from arachidonic acid. This makes acetylsalicylic acid different from other NSAIDS (such as diclofenac and ibuprofen) which are reversible inhibitors.

 

Summary of Analytical Methods for Aspirin
Official Methods for Aspirin6-8

SR.

No.

Official in

METHOD

BRIEF INTRODUCTION

REF.

NO

1

IP-2010

(Aspirin tablet)

Potentiometric Titration

Titrate:-

Tablet Powder Equivalent to 0.5 gm. Aspirin in 30ml of the 0.5M Sodium Hydroxide

Titrant:-

 0.5 M HCl

 

1ml of 0.5M NaOH is Equivalent to .0.04504 gm. of Aspirin

6

2

BP-2009

Potentiometric Titration

Titrate:-

1gm Aspirin in 10ml Ethanol, Add50ml of the 0.5M Sodium Hydroxide

Titrant:-

 0.5 M HCl

 

1ml of 0.5M NaOH is Equivalent to .0.04504 gm. of Aspirin

7

3

USP30-NF25

(Aspirin Tablet)

Liquid Chromatography

Mobile phase:-

Water (pH 3.4): Acetonitrile (85:15)

Column:-

Packing L1,  (300 mm × 4.0 mm)

Flow rate:-

 2 ml/min

Wavelength:-

 285 nm.

8

Reported Methods for Aspirin14-26

SR.

No.

DRUGS

METHOD

BRIEF INTRODUCTION

REF.

NO

1

Aspirin

RP-HPLC

Mobile phase:-

Sodium Perchlorate Buffer, pH (2.5): Acetonitrile: Isopropyl alcohol (85:14:1)

Column:-

C18, (100 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.5 ml/min

Wavelength:-

 275 nm.

9

2

Aspirin and Metoprolol

RP-HPLC

Mobile phase:-

Phosphate Buffer, (pH 4.6): Methanol (20:80)

Column:-

Phenomenex Luna C18, (250 mm × 4.6 mm, 5µ,)

Flow rate:-

 0.8 ml/min

Wavelength:-

 230 nm.

10

3

Aspirin, Ramipril,

Hydrochlorothiazide, Simvastatin And Atenolol

RP-HPLC

Mobile phase:-

Methanol: Water (95:5)

Column:-

 Hypersil Gold C18, (250 mm × 4.6 mm, 5µ,)

Flowrate:-

 1 ml/min

Wavelength:-

 230 nm.

11

4

Aspirin and

Prasugrel

RP-HPLC

Mobile phase:-

Acetonitrile: Acetate Buffer (75:25)

Column:-

 Luna C18 (150 mm × 4.6 mm, 5µ,)

Flow rate:-

 0 .6 ml/min

Wavelength:-

 245 nm

12

5

Aspirin and

Prasugrel

Stability indicating RP-HPLC

Mobile phase:-

Acetonitrile: Methanol: Water pH (3) (30:10:60)

Column:-

 Kromasil-100 C18 (150 mm × 4.6 mm, 5µ,)

Flow rate:-

 1 ml/min

Wavelength:-

 245 nm

13

6

Aspirin and Salicylic acid

RP-HPLC

Mobile phase:-

 Acetonitrile: Trifluoroacetic acid 0.05%  (30:70)

Column:-

 Waters C18 (250 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 230 nm

14

7

Amlodipine Besylate, Atenolol

and Aspirin

RP-HPLC

Mobile phase:-

 Methanol: Phosphate Buffer (pH 7.0) (70:30)

Column:-

 BDS C18 (250 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 235 nm

15

8

Aspirin And Aspirin Derivatives

RP-HPLC

Mobile phase:-

 Acetonitrile: Water (60:40)

Column:-

 Kromasil C18 (180 mm × 4.6 mm, 5µ,)

Flowrate:-

 1.0 ml/min

Wavelength:-

 277 nm

16

9

Aspirin, Caffeine and

Orphenadrine citrate

RP-HPLC

Mobile phase:-

 Methanol: Phosphate Buffer, pH3 (65:35)

Column:-

 Acclaim C18 (250 mm × 4.6 mm, 5µ,)

Flowrate:-

 1.0 ml/min

Wavelength:-

 215 nm

17

10

Aspirin and

Dipyridamole

RP-HPLC

Mobile phase:-

 0.1 % Phosphoric acid: Acetonitrile (75:25)

Column:-

 RP C18 (50 mm × 4.6 mm, 3.5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 227 nm

18

11

Aspirin and Esomeprazole

Magnesium

RP-HPLC

Mobile phase:-

 Acetonitrile: Methanol: Phosphate Buffer, pH 3.0 (25:25:50)

Column:-

 ODS BP C18 (200 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 230 nm

19

12

Ramipril, Aspirin and Simvastatin

RP-HPLC

Mobile phase:-

 Acetonitrile: Methanol: 0.5% phosphoric acid (10:70:20)

Column:-

 ODS BP C18 (200 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 226 nm

20

13

Aspirin,

Salicylic Acid, and Caffeine

RP-HPLC

Mobile phase:-

 Water: Methanol: Acetic acid (69:28:3)

Column:-

 Hypersil C18 (150 mm × 4.6 mm, 5µ,)

Flow rate:-

 1.0 ml/min

Wavelength:-

 275 nm

21

Clopidogrel Bisulphate[22-23]
Clopidogrel Bisulphate is an antiplatelet agent which is used to inhibit the aggregation of platelets which inhibits the blood clots  .The drug is Methyl (+)-(S)-α-(2-chlorophenyl)-6,7dihydrothieno [3,2-c] pyridine-5(4H) acetate sulfate. Clopidogrel bisulphate is insoluble in water.Clopidogrel Bisulphate is an irreversible inhibitor of P2Y12. The molecular formula of clopidogrel bisulphate is C16H16ClNO2S.H2SO4 and the molecular mass is 419.03 g/mol.

Pharmacological Action [23]
Clopidogrel is an anti-platelet agent which acts by direct inhibition of ADP. The anti –aggregating activity of the clopidogrel bisulphate is due to the biotransformation of the drug to 2-oxo-clopidogrel by enzyme P450-1A.Clopidogrel Bisulphate is mostlyused in the myocardial infarction, stroke and peripheral artery disease

Summary of Analytical Methods for Clopidogrel Bisulphate
Official Methods for Clopidogrel Bisulphate[24-25]

SR. NO

OFFICIAL IN

METHOD

DESCRIPTION

REF. NO

  1

IP 2010

(Clopidogrel Tablet)

Chiral Chromatography

Mobile phase:-

Phosphate Buffer: Acetonitrile (75:25)

Column:-

 Chiral Recognition Protein (15 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  220 nm

24

2

USP30-NF25

( Clopidogrel Tablet)

Chiral Chromatography

Mobile phase:-

Phosphate Buffer: Acetonitrile (75:25)

Column:-

 Packing L57 (15 cm X 4.6 mm)

Flow Rate:-

1.0 ml/min

Wavelength:-

  220 nm

25

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Reported Methods for Clopidogrel Bisulphate [26-39]

SR NO.

DRUGS

METHOD

            BRIEF INTRODUCTION

REF. NO.

1

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 2.8: Acetonitrile (35:65)

Column:-

 Develosil ODS (15 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  225 nm

26

2

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 4.0: Acetonitrile (32:68)

Column:-

 Hypersil BDS C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  220 nm

27

3

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Phosphate Buffer: Acetonitrile, Methanol (10:80:10)

Column:-

 Knauer C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

0.9 ml/min

Wavelength:-

  240 nm

28

4

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 3.0: Acetonitrile

(40:60)

Column:-

 C18 (15 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  224 nm

29

5

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

0.1% Trifluoroacetic acid: Acetonitrile

(30:70)

Column:-

 Inertsil ODS C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  220 nm

30

6

Clopidogrel

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 3.0: Acetonitrile

(75:25)

Column:-

 ODS C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 247 nm

31

7

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 8.0: Acetonitrile

(30:70)

Column:-

 Nova pack C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

0.8 ml/min

Wavelength:-

  210 nm

32

8

Clopidogrel Bisulphate

Stability Indicating RP-HPLC

Mobile phase:-

Tetrabutyl ammonium Hydrogen Sulfate Buffer: Acetonitrile

(70:30)

Column:-

 Symmetry C8 (15 cm X 3.9 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  225 nm

33

9

Clopidogrel Bisulphate

Stability Indicating RP-HPLC

Mobile phase:-

Phospahte Buffer, pH 4.0: Acetonitrile

(80:20)

Column:-

 C18 (15 cm X 4.6 mm),5 µm

Flow Rate:-

0.5 ml/min

Wavelength:-

  235 nm

34

10

Clopidogrel Bisulphate and Atorvastatin Calcium

 RP-HPLC

Mobile phase:-

Acetonitrile: Water (65:35)

Column:-

 Sphere-100 C18 (25 cm X 4.6  mm),5 µm

Flow Rate:-

0.5 ml/min

Wavelength:-

  227 nm

35

11

Clopidogrel Bisulphate and Atorvastatin Calcium

 RP-HPLC

Mobile phase:-

Solvent A: 0.1% Trifluoro acetic acid in water

Solvent B: 0.1% Trifluoro acetic acid in Acetonitrile

Column:-

 X-Bridge C18 (15 cm X 4.6  mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 215 nm

36

12

Clopidogrel Bisulfate,

Its Carboxylic Acid Metabolite, and Atorvastatin

 RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 2.6: Acetonitrile: Methanol

Column:-

 Hypersil BDS C18 (25 cm X 4.6  mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 220 nm

37

13

Phenytoin Sodium and

Clopidogrel Bisulphate

 RP-HPLC

Mobile phase:-

Water, pH 3.0: Acetonitrile (30:70)

Column:-

 Phenomenx Luna C18 (15 cm X 4.6  mm),5 µm

Flow Rate:-

0.5 ml/min

Wavelength:- 215 nm

38

14

Clopidogrel And Pioglitazone

 RP-HPLC

Mobile phase:-

Water, pH 4.6: Acetonitrile: Methanol (10:10:80)

Column:-

 C18 (15 cm X 4.6  mm),5 µm

Flow Rate:-

1 ml/min

Wavelength:- 230 nm

39

Rosuvastatin Calcium[40]
Rosuvastatin calcium is referred  as statin which is a cholesterol lowering drug .The IUPAC name of rosuvastatin is [(E)-7-[4-(4-fluorophenyl)-6-isopropyl-2-[methyl-(methylsulfonyl) amino] pyrimidin-5-yl] (3R, 5S)-3, 5-dihydroxyhept-6-enoicacid][40].The chemical formula of rosuvastatin calcium isC22H27FN3O6S)2.Ca and molecular mass of rosuvastatin calcium is 1001.1 g/mol.

Pharmacological Action[41]
Rosuvastatin is lipid lowering agent which inhibits the HMG-CoAwhich prevents the conversion of 3-hudroxy -3-methylglutaryl-coenzyme-A to melvonate which is precursor of cholesterol [41].Rosuvastatin is also used in the treatment of atherosclerosis, heartattack, stock and peripheral vascular disease.

Summary of Analytical Methods of Rosuvastatin Calcium

Official Methods of Rosuvastatin Calcium[42]

SR. NO

OFFICIAL IN

METHOD

DESCRIPTION

REF. NO

  1

IP 2010

(Rosuvastatin  Tablet)

 RP-HPLC

Mobile phase:-

Acetate Buffer, pH 4.0: Acetonitrile: Tetrahydrofuran (59:36:5)

Column:-

 C18 (25 cm X 4.6  mm),5 µm

Flow Rate:-

1.5 ml/min

Wavelength:- 248 nm

42

Reported Methods for Rosuvastatin Calcium [43-57]

SR NO.

DRUGS

METHOD

            BRIEF INTRODUCTION

REF. NO.

1

Rosuvastatin Calcium

RP-HPLC

Mobile phase:-

Water, pH 3.5: Acetonitrile (60:40)

Column:-

 YMC C8 (15 cm X 4.6 mm),5 µm

Flow Rate:-

1.5 ml/min

Wavelength:-

  242 nm

43

2

Rosuvastatin Calcium

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 3.0: Acetonitrile (50:50)

Column:-

 Thermo Hypersil C18 (10 cm X 4.6 mm),5 µm

Flow Rate:-

0.5 ml/min

Wavelength:-

  243 nm

44

3

Rosuvastatin Calcium

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 4.5: Acetonitrile: Methanol (50:50)

Column:-

 Luna C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  248 nm

45

4

Rosuvastatin

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 6.8: Acetonitrile: (60:40)

Column:-

 RP C18 (10 cm X 4.6 mm),3 µm

Flow Rate:-

0.6 ml/min

Wavelength:-

  242 nm

46

5

Rosuvastatin Calcium

RP-HPLC

Mobile phase:-

Acetonitrile: Water (75:25)

Column:-

 Enable C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-0.6 ml/min

Wavelength:-  252 nm

47

6

Rosuvastatin Calcium

Stability indicating RP-HPLC

Mobile phase:-

Solvent-A: Acetonitrile: Water: Methanol: Tetrahydrofuran (10:40:2:5)

Solvent-B: Acetonitrile: Methanol: Tetrahydrofuran (50:5:0.5)

Column:-

 Luna C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

2.0 ml/min

Wavelength:-

  243 nm

48

7

Rosuvastatin Calcium and Ezetimibe

RP-HPLC

Mobile phase:-

Acetonitrile: Water (75:25)

Column:-

 Enable C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-0.6 ml/min

Wavelength:-  252 nm

49

8

Rosuvastatin and Ezetimibe

RP-HPLC

Mobile phase:-

Phosphate Buffer: Acetonitrile: Methanol  (40:15:45)

Column:-

 Zorbax C18 (15 cm X 4.6 mm),3.5 µm

Flow Rate:-1.5 ml/min

Wavelength:-  242 nm

50

9

Rosuvastatin and Ezetimibe

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 8.0: Acetonitrile: Water  (50:40:10)

Column:-

 Waters C18 (25 cm X 4.6 mm),3.5 µm

Flow Rate:-1.0 ml/min

Wavelength:-  230 nm

51

10

Rosuvastatin and Ezetimibe

Stability indicating RP-HPLC

Mobile phase:-

Acetate Buffer, pH 6.5: Acetonitrile (55:45)

Column:-

 Sunfire BDS C18 (25 cm X 4.6 mm),3.5 µm

Flow Rate:-0.8 ml/min

Wavelength:-  230 nm

52

11

Rosuvastatin and Fenofibrate

RP-HPLC

Mobile phase:-

Water, pH 2.5: Acetonitrile (30:70)

Column:-

 Inertsil ODS C18 (25 cm X 4.6 mm),3.5 µm

Flow Rate:-1.0 ml/min

Wavelength:-  248 nm

53

12

Rosuvastatin Calcium and Fenofibrate

RP-HPLC

Mobile phase:-

Water: Acetonitrile: Methanol (20:40:40)

Column:-

 Agilent ODS C18 (25 cm X 4.6 mm),3.5 µm

Flow Rate:-1.0 ml/min

Wavelength:-  252 nm

54

13

Rosuvastatin Calcium and Fenofibrate

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 5.5: Methanol (25:75)

Column:-

 Phenomenex C18 (25 cm X 4.6 mm),3.5 µm

Flow Rate:-1.0 ml/min

Wavelength:-  272 nm

55

14

Rosuvastatin Calcium and Niacin

RP-HPLC

Mobile phase:-

Phosphate Buffer: Acetonitrile (50:50)

Column:-

 Inertsil ODS C18 (15 cm X 4.6 mm),3.5 µm

Flow Rate:-1.0 ml/min

Wavelength:-  254 nm

56

15

Rosuvastatin calcium and

Amlodipine besylate

RP-HPLC

Mobile phase:-

Acetonitrile: Tetrahydrofuran and Water, pH 3.0 (68:12:20)

Column:-

 Qualisil C8 (25 cm X 4.6 mm),3.5 µm

Flow Rate:-1.0 ml/min

Wavelength:-  251 nm

57

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REPORTED METHOD FOR ASPIRIN, CLOPIDOGREL BISULPHATE AND ROSUVASTATIN CALCIUM WITH EACH OTHER 58-67

SR NO.

 

DRUGS

METHOD

            BRIEF INTRODUCTION

REF. NO.

1

 

Aspirin and Clopidogrel

RP-HPLC

Mobile phase:-

3% o-Phosphoric acid: Acetonitrile (65:35)

Column:-

 Phenomenex C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  266 nm

58

2

 

Aspirin and Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Acetonitrile: Phosphate Buffer, pH 3.0: Methanol (50:30:20)

Column:-

 C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.5 ml/min

Wavelength:-

  240 nm

59

3

 

Aspirin and Clopidogrel

Stability indicating RP-HPLC

Mobile phase:-

Solvent A: Phosphate Buffer, pH 2.3

Solvent B: Methanol: Acetonitrile (50:50)

Column:-

 Phenyl Hexyl (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  220 nm

60

4

 

Aspirin and Clopidogrel Bisulphate

Ion pair RP-HPLC

Mobile phase:-

Acetonitrile: Tetrabutylammonium Hydrogen Sulphate Buffer (50:50)

Column:-

 Lichrosphere-100 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:-

  240 nm

61

5

 

Aspirin, Atorvastatin Calcium and

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Acetonitrile: Phosphate Buffer, pH 3.0 (50:50)

Column:-

 Inertsil ODS (15 cm X 4.6 mm),5 µm

Flow Rate:-

1.2 ml/min

Wavelength:-

  235 nm

62

6

 

Aspirin, Atorvastatin Calcium and

Clopidogrel Bisulphate

RP-HPLC

Mobile phase:-

Acetonitrile: Water, pH 3.0:Methanol (50:40:10)

Column:-

 Hypersil BDS C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 248 nm

63

7

 

Aspirin and Rosuvastatin

RP-HPLC

Mobile phase:-

Methanol: Buffer (45:55)

Column:-

 X-Terra C18 (15 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 215 nm

64

8

 

Aspirin and Rosuvastatin Calcium

RP-HPLC

Mobile phase:-

Water with 0.5% Triethylamine: Acetonitrile (50:50)

Column:-

 Smart C18 (25 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 243 nm

65

9

 

Aspirin and Rosuvastatin Calcium

RP-HPLC

Mobile phase:-

Phosphate Buffer, pH 3.0: Acetonitrile (45:55)

Column:-

 Hyper chrome ODS BP (20 cm X 4.6 mm),5 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 241 nm

66

10

 

Clopidogrel Bisulphate and Rosuvastatin Calcium

RP-HPLC

Mobile phase:-

Perchlorate Buffer, pH 2.5: Acetonitrile (65:35)

Column:-

 Nova pack C18 (10 cm X 3.9 mm),4 µm

Flow Rate:-

1.0 ml/min

Wavelength:- 242 nm

67

CONCLUSION
Aspirin, clopidogrel bisulphate and rosuvastatin calciumplay an important role in the many cardiovascular diseases, and in various diseases .These drugs are available in the market in many formulations with their different dose .Many methods have been reported for the estimation of these drugs but currently not any method have been reported for the simultaneous estimation of these drugs in their combined dosage form. So there is need to develop a suitable, accurate and validated method for their simultaneous estimation in combined dosage form.

REFERENCE
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6. Indian Pharmacopeia-2010, Indian Government Health and Welfare Society, Ghaziabad,    pp843.
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8. USP30-NF25, Pharmacopeia Forum: Volume No. 29(6) pp 1446.
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