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A REVIEW OF ANALYTICAL METHODS FOR DETERMINATION BROMHEXINE HYDROCHLORIDE IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
Meera V. Lad1*, Vineet Jain2, Hasumati Raj1
1Department of Quality Assurance,
2Department of Pharmacognosy,
Shree Dhanvantary Pharmacy College, Kim, Gujarat
Bromhexine HCl (BRH)is a mucolytic agent used in the treatment of respiratory disorders associated with viscid or excessive mucus, chemically named 2-amino-3,5-dibromo-N-cyclohexyl-N-methyl benzenemethanamine hydrochloride. According to IUPAC it is 2,4-dibromo-6-[[cyclohexyl(methyl)amino]methyl] aniline hydrochloride. Because of its physiological importance, the drug has been quantified by exploiting its chemical and physical properties. Bromhexine is a weak base and its precipitate out at pH value above 6. Bromhexine is a synthetic benzyl amine derivative ofvasicine. The different analytical methods used to quantify the drug as a single active pharmaceutical ingredient include flow injection analysis with ionselectiveelectrodes, inductively coupled plasma mass spectrometry, electrokinetic chromatography, electrochemical oxidation at the glassy carbon electrode, liquid chromatography, liquid gas chromatography, GC with mass detection, and voltammetry. The drug has also been quantified in its combined formulations using HPLC, direct and derivative UV spectrophotometry.
A REVIEW: ANALYTICAL METHODS FOR DETERMINATION OF CILNIDIPINE IN BIOLOGICAL FLUID AND PHARMACEUTICAL DOSAGE FORMS
Farhana V. Buchiya*, Vineet Jain, Hasumati Raj
Shree Dhanvantary Pharmacy College,
Kim, Surat, Gujarat
Cilnidipine is act as a dual blocker by blocking L- type of calcium channel present in vascular smooth muscles and N- type of calcium channel present in sympathetic nerve terminal that supply blood vessels. Cilnidipine used in treatment of mostly in hypertension and various cardiovascular diseases except in Angina. Cilnidipine used alone or in combination. This review covers most recent analytical methods such as various spectroscopic methods, chromatographic methods and other methods for determination of cilnidipine in various pharmaceutical dosage forms and biological matrix were reported.
Amitava Sinha Ray
Ranbaxy Laboratories Ltd
West Bengal, India
Cancer is basically a disease of cells characterized by a shift in the control mechanism which is associated with cell differentiation. In most cases causes of cancer is multifactorial. Cancer is the second most causes of death after heart disease. Cytotoxic chemotherapy for cancer offers cure for only certain type of cancer. Chemotherapy may prolong the life. Chemotherapy provides palliative rather than curative therapy at present.
DEVELOPMENT OF HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD FOR THE DETERMINATION OF SCOPOLIN IN CONVOLVULUS PLURICAULIS CHOIS
Department of Pharmacognosy,
Indo-Soviet Friendship College of Pharmacy, Moga, Punjab, India.
A simple, selective, precise and robust high performance thin layer chromatography (HPTLC) method was developed and validated for the determination of scopolin, in Convolvulus pluricaulis, commonly known as shankhpushpi. Aluminium backed pre-coated TLC plates (Silica gel 60F-254) of 0.2 mm thickness, were utilized to perform chromatography. The plates were developed up to 90 mm distance in twin trough glass chamber, saturated for 10 min with mobile phase system (Chloroform: methanol, 8.5:1.5, v/v). The system produces a sharp and well-defined peak for scopolin (Rf value; 0.30 ± 0.02). Scopolin was quantified in various test samples (extracts and marketed formulation) at absorbance maxima of 340 nm with the help of CAMAG TLC scanner III. The linear regression analysis data for the calibration plots (concentration Vs peak area) showed good linearity (r2 = 0.9992 ± 0.0002) in the concentration range of 20-160 ng spot−1. The method was validated in accordance with International Conference on Harmonization (ICH) guidelines. The statistical analysis of the results, indicate that the proposed method is specific, accurate and precise. Further, this method can be used for the standardization of shankhpushpi and its formulations.
Goundla Uday Bhasker Goud1*, Jakkampudi Sri Venu Prakash1, Avadhanam Pranav Kumar2, Gangi Reddy Sreenivas Reddy2, Manikanta Sai Krishna2.
1Department of Industrial Pharmacy
2Department of Pharmaceutics
Bharat Institute Of Technology, Mangalpally, Hyd, Telangana, India.
Aim: The current paper was an attempt to design a extended release dosage form of Metformin hydrochloride using various grades of hydrophilic polymers, hydroxy propyl methyl cellulose (HPMC K4M, HPMC K15M, HPMC K100M and HPMC K200M) and MCC.
Materials and Methods: Laboratory scale batches of 4 tablet formulations were prepared by wet granulation technique. Precompression parameters of the granules were evaluated prior to compression. Tablets were characterized as crushing strength, friability, weight variation, thickness, drug content or assay and evaluated for in-vitro release pattern for 12 hr using Phosphate buffer of pH 6.8 at 37 ± 0.5°C.
Results and Discussion: The results obtained revealed that HPMC K100M in formulation (F3) was able to sustain the drug release for 12 h and followed the Higuchi pattern quasi-Fickian diffusion. and charged for stability testing, parameters were within the limit of acceptance. There was no chemical interaction found between the drug and excipients during Fourier Transform Infrared Spectroscopy (FTIR).
Conclusion: Hence it can be concluded that formulation F3 containing HPMC K100M is suitable for development of extended release tablets of Metformin Hydrochloride.
DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF SILDENAFIL CITRATE AND DAPOXETINE HYDROCHLORIDE IN THEIR COMBINED DOSAGE FORMULATION
Chetan A. Prajapati, Bhavik S. Patel
Department of Quality Assurance, Shri Sarvajanik Pharmacy College
Nr. Arvind Baug, Mehsana, Gujarat, India
A simple, accurate and precise spectrophotometric method has been developed for simultaneous estimation of Sildenafil Citrate and Dapoxetine Hydrochloride in combined dosage form. Simultaneous equation method is employed for simultaneous determination of Sildenafil Citrate and Dapoxetine Hydrochloride from combined dosage forms. In this method, the absorbance was measured at 291 nm for Sildenafil Citrate and 230nm for Dapoxetine Hydrochloride. Linearity was observed in range of 6-42μg/ml and 2-10μg/ml for Sildenafil Citrate and Dapoxetine Hydrochloride respectively. Recovery studies confirmed the accuracy of proposed method and results were validated as per ICH guidelines. The method can be used for routine quality control of pharmaceutical formulation containing Sildenafil Citrate and Dapoxetine Hydrochloride.
Prakash Chanda Gupta
Quality Control Executive,
National Healthcare Pvt. Ltd, Nepal
The science of physics is understood to be very away from the Pharmaceutical Science. But in the real context, Physics is found to be most densely involved in the Pharmaceutical Science than any other field of science. Physics can be considered as a backbone that supports Pharmaceutical Science. The advance technology that describes that formation and its mode of action to the biological organ is Physics. The structure, action and result of Pharmaceutical material are explained by the theories of Physics.
Patel Divya A*., Varodiya Priyanka S., Raj Hasumati A.
Department of Quality Assurance, Shree Dhanvantary Pharmacy College,
Kim, Surat, Gujarat, India
This review article presents the pharmacology of combined edaravone and argatroban therapy specialy in acute ischemic stroke. Edaravone (MCI-186) is a free radical scavenger, a novel neuroprotective agent. Argatroban is a selective thrombin inhibitor.The antithrombotic agent was used in acute cerebral infarction. If the antithrombotic agent is administered in large quantities, the condition of patient become worse by occurrence of adeverse effect of cerebral haeorrhage. The use of edaravone in combination with antithrombotic agent has been proved to provide beneficial effect in acute ischemic stroke as edaravone has no influence to coagulation of blood and platelets aggregation. The combination therapy has fewer hemorrhagic adeverse effect. The mechanism of argatroban and edaravone is quite different. Argatroban, an anti-coagulant drug, directly improves the microcirculation of ischemic brain tissue while edaravone could indirectly attenuated brain edema by protection of endothelial cells damaged by free radicals generated after ischemic insult. The combination of both would have reciprocal and enhanced neuroprotective effects against ischemic insult. Both the drugs were approved by Japanese government and has been used in acute brain infarction in japan. The main objective of this review article is to provide pharmacological information of combined therapy of edaravone and argatroban to researcher in development of combined dosage form of this.
Madhusudan P Dabhole
Group Manager – BioProcess,
Richcore Life Sciences Ltd, Bangalore, Karnataka, India
The manufacturing of recombinant products by fermentation and purification in stainless steel vessels has seen the transition from small scale to large scale and further to single use disposable technology. The requirement to develop and modulate the process has arisen from the cost and manufacturers need to move the facility on mobile platforms. The review describes the strategies and considerations for Single Use Disposable Technology. Recombinant proteins are widely used for treatment of various diseases and disorders. Single Use Disposable Technology makes it promising to produce and formulate these proteins from bench scale to commercial level in a shorter span of time so that it can reach the physician and patients.
Amitava Sinha Ray
Ranbaxy Laboratories Ltd
West Bengal, India
Anticoagulantis an agent that is used to prevent the formation of blood clots. Anticoagulants, such as heparin or warfarin work on chemical reactions in the body to lengthen the time it takes to form a blood clot. Heparin the anticoagulant drug that is used to prevent blood clots from forming during and after surgery and to treat various heart, lung, and circulatory disorders in human body. Heparin is comparatively a strong acid that forms water–soluble salts. It is used in treatment of venous thrombosis and its extensions, pulmonary embolism (PE), peripheral arterial embolism. Smoking and alcohol may alter response to heparin. Abrupt withdrawal of heparin may precipitate increased coagulability.