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DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF TRIAMTERENE AND BENZTHIAZIDE IN TABLETS

ABOUT AUTHORS:
VC Chauhan*, VN Shah, DA Shah, RR Parmar
Department of Quality Assurance
APMC College of Pharmaceutical Education and Research,
Motipura, Himmatnagar, Gujarat 383001
vikas14vks@gmail.com

ABSTRACT
A  specific,  accurate,  precise  and  reproducible  RP-HPLC  method  has  been  developed  and subsequently validated for the simultaneous determination of Triamterene and Benzthiazide in tablets. The proposed HPLC method utilizes BDS hypersil (Thermo scientific) C18 column (250 mm × 4.6 mm id, 5 μm particle size), and mobile phase consisting of phosphate buffer: methanol (70:30) and pH adjusted to 3.5 with sodium hydroxide and flow rate of 1.0 ml/min. Quantitation was achieved with UV detection at 245 nm based on peak area with linear calibration curves at concentration ranges 10-30 μg/ml for Triamterene and 5-15 μg/ml for Benzthiazide. The retention time of Triamterene and Benzthiazide were found to be 5.960 min and 3.493 min respectively.  The  method  was  validated  in  terms  of  accuracy,  precision,  linearity,  limits  of detection,  limits  of  quantitation  and  robustness.  This  method  has  been  successively  applied  to tablet formulation  and  no interference  from the  formulation excipients  was found.


PREPARATION AND EVALUATION OF MATRIX TABLETS CONTAINING AMBROXOL HYDROCHLORIDE

ABOUT AUTHORS:
Soma Vinisha*, Sajida Akhtari Begum, Nikhat Tabassum, Soma Anusha
Department of pharmaceutical science,
Bharat Institution of Techology, Hyderabad, India
choti.reddy@gmail.com

ABSTRACT
Purpose:
This study aimed to formulate Ambroxol hydrochloride SR matrix tablets using xanthan gum (natural polymer) and to elucidate the release kinetics of Ambroxol hydrochloride from xanthan gum-matrices. Methods: controlled release matrix tablets of ambroxol hydrochloride, a mucolytic expectorant  were prepared by wet granulation method using xanthan gum as natural hydrophilic polymer in three different ratios (Drug : Polymer 1:1(F-1), 1:1.5(F-2), 1:2(F-3)). The prepared granules of three different formulations were evaluated for angle of repose, bulk density (BD), tapped density (TD) and compressibility index (CI), hausners ratio. The prepared tablets were tested for physical parameters like weight variation, hardness, friability, content of active ingredient and In-vitro drug release studies. Results: The results obtained were within the prescribed limits. The release of ambroxol from the matrix tablets was sustained up to 12hrs. The cumulative percentage of drug release was decrease with increase in polymer concentration. Among the three formulations F-3 gave the release profile close to the commercially available marketed sample of ambroxol Hcl (A-MS).The results indicate that the drug release from the matrix tablets followed Zero order kinetics. The dissolution data was fitted to Korsmeyer equation which is used to describe the drug release behaviour from polymeric systems. All the formulations showed diffusion co-efficient value (n) greater than 0.43 but less than 0.85 after fitting to the Korsmeyer equation. So, it indicates Non-Fickian transport mechanism. Therefore the drug release is by diffusion and erosion mechanism. Conclusion: Matrix tablets of Ambroxol Hydrochloride using xanthan gum prepared by wet granulation method were found to be good in appearance. The drug-polymer ratio was found to influence the release of drug from the formulations. Formulation F-3 i.e. (1:2 drug: polymer) exhibited the similar In-vitro drug release rates as that of the marketed sample.


IMPACT OF OBESITY ON PULMONARY FUNCTION TEST IN SCHOOL CHILDREN

About Authors:
Madhuri Sharma*1, KC Mathur1, Rajnee2, Raaz K Maheshwari3
1Department of Physiology, Sardar Patel Medical College, Bikaner, Rajasthan
2Department of Physiology, Sarojini Naidu Medical College, Jodhpur, Rajasthan
3Department of Chemistry, SBRM Govt PG College, Nagaur, Rajasthan
*madhurisharma26@gmail.com

Abstract
Pulmonary function test are affected by the obesity. Various studies demonstrate important modifications in the pulmonary function tests of obese adults and adolescents, there is scarce information on obese children, and the findings remain inconclusive. Although there are some studies which demonstrate important modifications in the respiratory pattern of obese children, but there are some contradictory findings. So such type of study should be done which give us different and separate findings for overweight, mild obese, moderately obese, and morbidly obese criteria in obese school children in comparison with age, sex and ethnically matched control group.


ANTIULCER ACTIVITY OF ANNONA SQUAMOSA SEED EXTRACT IN EXPERIMENTAL RATS

About Authors:
Pankaj Kushwah1*, Nandu Kayande2
1Department of Pharmacognosy
2HOD Department of Pharmacology
Nimar Institute of Pharmacy, Dhamnod,
Opp.ITI, Maheshwar Road Dhamnod, Dhar. M.P.454552
Pankajnipd@gmail.com

Abstract
Antiulcer activity of aqueous extracts of  Annona squamosa seed extract was studied in Rats by pyloric ligation method. The  extract at dose of 100,200 mg/kg produced significant inhibition of gastric lesion induced by Pylorus ligation induced ulcer. The parameters taken to assess anti-ulcer activity were volume of gastric secretion, PH, free acidity, total acidity and ulcer index. The results indicate  that the alcoholic extract significantly (P  < 0.001) decreases the volume of gastric acid secretion, PH, free acidity, total acidity and ulcer index with respect to control.


ABSORPTION CORRECTION SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF TORSEMIDE AND AMILORIDE HYDROCHLORIDE IN THEIR COMBINED DOSAGE FORM

ABOUT AUTHORS:
Dilip G. Maheshwari, Rujuta D. Patel*
Department of Quality Assurance,
L.J. Institute of Pharmacy, Sarkhej, Ahemdabad, Gujarat, India
*Rujutapatel09@gmail.com

ABSTRACT:
A new sensitive, simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Torsemide and Amiloride HCl in pharmaceutical dosage form. This method was based on UV spectrophotometric determination of two drugs, using absorbance correction method. It involves measurement of absorbances at two wavelengths 288nm(λmax of Torsemide (TOR)) and 361nm (λmax of Amiloride HCl (AMI)) in methanol for the simultaneous quantitative determination of Torsemide and Amiloride HCl in the binary mixture without previous separation. The linearity was observed in the concentration range of 5 -25 μg/ml for Torsemide and 5-25 μg/ml for Amiloride HCl. The accuracy and precision of the method was determined and validated statically. The method showed good reproducibility and recovery with % RSD less than 2. Method was found to be rapid, specific, precise and accurate, can be successfully applied for the routine analysis of Torsemide and Amiloride HCl in combined dosage form without any interference by the excipients. The method was validated according to ICH guidelines.


DIABETES AND ITS COMPLICATIONS AND SGLT-2 INHIBITORS: A NOVEL THERAPY FOR TYPE 2 DIABETES MELLITUS

About Authors:
Shivkant M. Kide*, Rahul M. Kide, Lokesh T. Thakare, Komal V. Ambulkar
Department of Pharmacology,
Institute of Pharmaceutical Education and Research,
Borgaon (Meghe), Wardha-442 001,(M.H.), India.
shivpharma08@gmail.com

Abstract:
The incidence of type 2 diabetes mellitus is increasing worldwide. The pharmacotherapy for type 2 diabetes until the last decade only consisted of biguanides, sulphonylureas, and insulin. The existing therapeutic classes of antidiabetic drugs are not adequately effective in maintaining long-term glycemic control in most patients, even when used in combination. One emerging novel therapeutic class of antidiabetic drugs is sodium glucose cotransporter 2 (SGLT2) inhibitors. SGLT2 accounts for 90% of the glucose reabsorption in the kidney. The SGLT2 inhibitors increase urinary excretion of glucose and lower plasma glucose levels in an insulin-independent manner. Forxiga (Dapagliflozin) the most prominent molecule in this class is currently approved by USFDA. Other members of this class (eg, sergliflozin, remogliflozin) are also effective. This class of novel agents can effectively control blood sugar level without producing weight gain or hypoglycemia.


FORCED DEGRADATION AND STABILITY TESTING: STRATEGIES AND ANALYTICAL PERSPECTIVES

ABOUT AUTHORS:
U. A. Deokate, A. M. Gorde*
Dept. Of Pharmaceutical Chemistry,
Government College of Pharmacy, Hotel Vedant Road, Osmanpura,
Aurangabad, Maharashtra, India 431005
gordeanjali@gmail.com

ABSTRACT:
This review discusses the regulatory aspects of forced degradation and methodology aspects for degradant investigations. It also focuses on the prediction of degradation products and pathways and development of stability indicating assay method. While reviewing the analytical perspectives various conventional and hyphenated techniques for degradant separation and characterization are described in detail.


PHARMACOLOGICAL SCREENING OF ETHANOLIC EXTRACTS OF EMBLICA OFFICINALIS GEARTH PLANT ON ANIMALS

ABOUT AUTHORS:
Nandu Kayande1*, Pankaj Kushwah2, D. K. Vir1
1Department of Pharmacology
2Department of Pharmacognosy
Nimar Institute of Pharmacy, Dhamnod,
Opp.ITI, Maheshwar Road Dhamnod, Dhar. M.P.454552
nandu_kayande@rediffmail.com

ABSTRACT
The main objective of the present work is to find out good pharmacological activities in herbal source with their preliminary phytochemical study, and also it is aimed to investigate of ethanol, and aqueous extracts of dried stem of plant Emblica Officinalis Gearthagainst with Anti-inflammatory and in rats, Analgesic activity in mice and antioxidant in invitro methods. Normally herbal products are free from side effects/adverse effects and they are low cost medicines,which will be beneficial for human being. The main objective of this work is to find active constituents which are potent Anti-oxidant, Anti-inflammatory, analgesic having no or minimum side effects from indigenous plants for the therapeutic management.


DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF BROMHEXINE HYDROCHLORIDE AND PHENYLEPHRINE HYDROCHLORIDE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM

About Authors:
Dharmesh R. Mevada*, Krutika Bhalodiya, Brijesh Maniar, Ketan Dadhania, Shital Faldu
Department of Quality Assurance,
Smt. R. D. Gardi B. Pharmacy College,
Rajkot, Gujarat, India
*dharmeshmevada89@gmail.com

Abstract
UV Spectrophotometric method has been developed for simultaneous estimation of Bromhexine HCl (BHX) and Phenylephrine HCl (PLE) in bulk drug and in their combined Pharmaceutical dosage form by first order derivative. This method utilizes methanol as a common solvent and λmax of BHX and PLE selected for analysis was found to be at 241 nm (at ZCP of PLE) and 233 nm (at ZCP of BHX) respectively. Linearity was observed in the concentration range of 5-30 μg/ml for BHX (r2 = 0.999) and 10-60 μg/ml for PLE  (r2 = 0.998). The accuracy and precision were determined and found to comply with ICH guidelines. This method showes good reproducibility and recovery with % RSD in the desired range. Developed method was applied for marketed formulation. The results were found to be within  acceptance criteria according to ICH guideline. This method was simple, rapid, accurate, Precise and sensitive.


FORMULATION AND EVALUATION OF SUSTAINED RELEASE TABLETS OF AMBROXOL HYDROCHLORIDE

ABOUT AUTHORS:
Samatha.M*, Deepthi.P, N. Srinivas
Department of pharmaceutics,
Malla Reddy Institute of Pharmaceutical Sciences, Secunderabad
samathamapashetti@gmail.com

ABSTRACT
In the present study, an attempt was made to formulate the oral sustained release matrix tablets of AmbroxolHCl in order to improve the efficacy, reduce the frequency of administration, and better patient compliance. Ambroxol Hydrochloride is a potent mucolytic agent which induces bronchial secretions used in the treatment of respiratory disorders. FTIR analysis confirmed the absence ofany drug polymer interaction. Sustained release tablets of Ambroxol Hydrochloride were formulated employing hydrophilic polymers HPMC K4M and HPMC K100M. The powder blend was evaluated for micromeritic properties. The sustained release tablets were prepared by wet granulation method. The tablets were evaluated for thickness, weightvariation test, hardness, friability, and drug content. The in vitro drug release characteristics were studied in simulated gastric fluid (2 hours) andintestinal fluid for a period of 10 hours using USP type II dissolution apparatus (total 12hours). The results of dissolution studies indicated that formulation F11 is the most successful of the study with satisfactory drug release. At the end of 12 hrs, the drug release was found to be 96.1%.