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ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND PROPYPHENAZONE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM

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About Authors:
Mehul Kakdiya*, Avinash Nagapara, Darshan Madiya, Shital Faldu
Department of Quality Assurance,
Smt. R. D. Gardi B. Pharmacy College, Rajkot, Gujarat, India
*kakdiyamehul@gmail.com

Abstract
A simple, precise, accurate and reproducible spectrophotometric method has been developed for  simultaneous estimation of Paracetamol (PCM) and Propyphenazone (PP) by employing first order derivative zero crossing method in Methanol. The first order derivative absorption at 249 nm (zero cross point of Paracetamol) was used for quantification of Propyphenazone and 274 nm (zero cross point of Propyphenazone) for quantification of Paracetamol. The linearity was established over the concentration range of 1-12 µg/ml and 5-24 µg/ml for Paracetamol and Propyphenazone with correlation coefficient r2 0.999 and 0.996, respectively. The mean % recoveries were found to be in the range of 100.48-102.119% and 100.448-102.713 % for Paracetamol and Propyphenazone, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Paracetamol and Propyphenazone in their combined pharmaceutical dosage form.


Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF PARACETAMOL AND PROPYPHENAZONE IN THEIR COMBINED PHARMACEUTICAL DOSAGE FORM

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About Authors:
1Mehul Kakdiya*, 2Viral Kakdiya, 1Darshan Madiya
1Department of Quality Assurance,
Smt. R. D. Gardi B. Pharmacy College, Rajkot
2Pacific college of pharmacy, Udaipur, Rajasthan
*kakdiyamehul@gmail.com

Abstract
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical Q-absorbance ratio method for the simultaneous determination of Paracetamol and Propyphenazone in their combined pharmaceutical dosage form. Absorbance ratio method uses the ratio of absorbance at two selected wavelengths, one which is an isoabsorptive point and other being the λ-max of one of the two components.  Paracetamol and Propyphenazone show an isoabsorptive point at 264 nm in methanol.The second wavelength used is 249 nm, which is the λ-max of Paracetamol in methanol. The linearity was obtained in the concentration range of 1-12 μg/ml for Paracetamol and 5-24 μg/ml for Propyphenazone. The concentrations of the drugs were determined by using ratio of absorbance at isoabsorptive point and at the λ-max of Paracetamol. The method was successfully applied to pharmaceutical dosage form because of no interference. The results of analysis have been validated by recovery studies.


NEW SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF LUMEFANTRINE

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ABOUT AUTHORS:
*1Baokar Shrikrishna, 1Annadate Amol, 2Undare Santosh
1Department of Pharmaceutical Chemistry, SVPM’s College of Pharmacy, Malegaon (Bk II), Tal-Baramati, Dist- Pune, Maharashtra, India
2P.G. Department of chemistry, Balbhim arts, science and commerce college, Beed
krishnabaokar@gmail.com

ABSTRACT
A Simple, sensitive, specific, spectrophotometric method has been developed for the detection of Lumefantrine in pure and Pharmaceutical formulations. The optimum condition for the analysis of the drug was established. Lumefantrine shows maximum absorption at 228 nm and obeyed beers law in the concentration range 10 to 50 µg/ml.

The correlation coefficient was found to be 0.999 and slope of line was found to be 0.0635. The percent S.D. for intra assay precision of the method was found to be 1.85% whereas Inter assay precision was found to be 0.44%. The sample solution was stable up to 24 hours. The assay results were found to be in good agreement with label claim.

The proposed method was simple sensitive, precise, quick and useful for routine quality control.


Q-ABSORBANCE RATIO SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE AND CANDESARTAN CILEXETIL IN SYNTHETIC MIXTURE

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ABOUT AUTHORS:
Kotecha B*, Pambhar M, Zala G, Faldu S
Department of Quality assurance, Smt. R.D. Gardi B.Pharmacy College,
Nyara, Rajkot-360 110, Gujarat, India
kotecha_bijal29@yahoo.com

ABSTRACTS:
The present manuscript describe simple, sensitive, rapid, accurate, precise and economical q-absorbance method for the simultaneous determination of Amlodipine besylate and Candesartan cilexetil in synthetic mixture. Absorbance ratio method uses the ratio of absorbance at two selected wavelengths, one which is an Isoabsorptive point at 242 nm in Methanol. The second wavelength is used 255 nm, which is λmax of Candesartan cilexetil in Methanol. The linearity was obtained in the concentration range of 5-25 μg/mlof both drugs. The concentration of drugs was determined by using ratio of absorbance at isoabsorptive point and at the λ-max of Candesartan cilexetil. The method was successfully applied to pharmaceutical dosage form because of no interference. The result of analysis has been validated by recovery studies.


UV-VISIBLE SPECTROSCOPY: AN OVERVIEW

ABOUT AUTHORS:
Kambham Venkateswarlu1*, N.Devanna2, Haritha Arikeri3, M.Shahinaz Farihath4
1,4M.Pharm Scholar, Department Of Pharmaceutics,
2Director Of JNTUA-Otri,
3M.Pharm Scholar, Department Of Pharmaceutical Analysis
JNTUA-Oil Technological Research Institute,
Beside Collector Office, Anantapur, Anantapur District, Andhra Pradesh, India. Pin Code: 515001
*k.v.reddy9441701016@gmail.com

ABSTRACT:
Spectroscopy is often used in physical and analytical chemistry for the identification of substances through the spectrum emitted from or absorbed by them. Spectroscopy is also heavily used in astronomy and remote sensing. Most large telescopes have spectrometers, which are used either to measure the chemical composition and physical properties of astronomical objects or to measure their velocities from the Doppler Shift of their spectral lines.
UV-Visible spectroscopy is a form of Absorption spectroscopy. Absorption spectroscopy in the UV-Visible region is to be one of the oldest and most frequently employed technique in pharmaceutical analysis for qualitative, quantitative and structural analysis of a substance in solution. The substance is analyzed by studying the spectrum produced by it due to absorption of certain wavelengths of UV-Visible light.
Spectroscopically, visible light behaves in a similar way as UV light. Hence, the techniques of UV spectroscopy and Visible spectroscopy are studied together.


A COMPLETE REVIEW ON NUCLEAR MAGNETIC RESONANCE (NMR)

ABOUT AUTHORS:
*Md. JahaSultana, P. Vijaya Sri, G.Alekhya, S.VenkateswaraRao, Chaitanya PrasadMeher
Department Of Pharmaceutical Analysis
Vijaya Institute of Pharmaceutical Sciences For Women
Enikepadu, Vijayawada-521108, Andhra Pradesh, India
sohnivya786@gmail.com

ABSTRACT:
Over a past decades, nuclear magnetic resonance (NMR) has progressed rapidly in improvement of experimental method and development of novel approaches. NMR is a research technique that exploits the magnetic properties of certain atomic nuclei. Of all the spectroscopy methods, NMR is the only one of which a complete analysis and interpretation of the entire spectrum is normally excepted. Although larger amounts of samples are needed when compared with mass spectroscopy, NMR is non-destructive and with modern instruments good data may be obtained from samples weighing less than a milligram. In this review a brief introduction to the NMR is given which includes instrumentation, parameters influencing NMR and applications.


A CRITICAL REVIEW ON PHARMACEUTICAL ANALYSIS OF NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY (HETCOR SPECTROSCOPY)

About Authors:
Reshma. K*, M.Muthukumaran*, B.krishnamoorthy, Amreen Nishat
Montessori Siva sivani Institute of Science&Technology- College Of
Pharmacy
Vijayawada, Andhrapradesh-521 230
reshmakaaja@gmail.com

Abstract
Nuclear magnetic resonance (NMR) has progressed rapidly over the last decade as a result of improved experimental technology and development of novel approaches. NMR spectroscopy has evolved into an important technique in support of structure-based drug design. It was most useful as a technique to provide structural information regarding protein drug targets and target–ligand interactions. More recently, it has been shown that NMR may be used as an alternative method for identification of small molecule ligands that bind to protein drug targets. High throughput implementation of these experiments to screen small molecule libraries may lead to identification of potent and novel lead compounds. NMR as a probe of microscopic dynamic behaviour through relaxation and direct diffusion measurements over a wide temperature range is examined.


ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

About Authors:
Jasinth. Danda*, Dr. P.Venkateswara Rao, P.Rama Bharathi, B.Madhavi, T.Swathi, Satya Lakshmi. Balla
A.M Reddy Memorial College of Pharmacy,
Department of Pharmaceutical Analysis
Narasaraopet, ANU University, Guntur.
Jasinthdanda18@gmail.com

1. Introduction
Chromatography is relatively a new technique which was first invented by M.Tsweet, a botanist in 1906 in Warsaw. He was successful in doing the separation of chlorophyll, xanthophylls and several other coloured substances by percolating vegetable extracts through a calcium carbonate. The calcium carbonate acts as absorbent and the different substances got adsorbed to different extents and this gives rise to coloured bands at different positions on the column. Tsweet termed this coloured bands as chromatogram and the method as chromatography after the Greek words chroma and graphos  meaning colour and writing respectively.


DEVELOPMENT AND VALIDATION OF IR SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF AZELASTINE HYDROCHLORIDE IN NASAL SPRAY PREPARATIONS

ABOUT AUTHORS:
*1Patel Rina B., 2Patel Nilam K.
1M.pharm(Q.A), Department of Pharmaceutical Sciences
Hemchandracharya North Gujarat University,
2Asst. Professor, Dept. of Pharmaceutical Sciences
Department of Pharmaceutical Sciences, Hemchandracharya North Gujarat University, Patan. Gujarat-384265
*rina_patel2003@yahoo.com

ABSTRACT
A simple, sensitive, rapid, accurate, precise and economical Fouriertransform infrared (FTIR) spectrophotometric method has been developed for the determination of Azelastine Hydrochloride (AZH)   in nasal spray preparations. The IR spectrophotometric method was based on the determination of AZH by the measurement of the area of the infrared band corresponding to the carbonyl group centred at the band1636 cm-1. It was present in the AZH but not in Acetonitrile used as solvent and highest intensity was at the area 1701.08-1584.21 cm-1. The linearity of AZH was obtained in the concentration range of 5-100 μg/ml. The mean % recovery was 99.67 ± 0.57 %. The recovery studies confirmed accuracy of proposed method and low values of standard deviation confirmed precision of method.


NOVEL RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF LOSARTAN POTASSIUM AND AMLODIPINE DRUGS IN PURE AND PHARMACEUTICAL DOSAGE FORMS

ABOUT AUTHORS:
1Kumari Jyothsna*, 2N.Chandana
1,2Department of Pharmaceutical Analysis and Quality Assurance,
Nimra College of Pharmacy, Jupudi, Vijayawada, A.P, India
jyothsna.s.k24@gmail.com

ABSTRACT
A fast, robust and accurate RP-HPLC method was developed and validated for simultaneous determination of Losartan potassium and Amlodipine in tablets. The mobile phase was mixture of aqueous Tri ethyl amine with pH 2.0 and Acetonitrile(70:30), effluent flow rate monitored at 1.0 ml/min. the stationary phase was C18 column, 3µm(4.6×250mm). The solutions of standard and the sample were prepared in methanol. The retention times was found to be 2.916min and 5.214min for Losartan potassium and Amlodipine respectively at 246nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of 50-150µg/ml. The percentage assay for Losartan potassium and Amlodipine were found to be 101% and 100%respectively. The method was validated and it was found to be accurate, precise, linear and reproducible as per ICH guidelines.


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