Development and Validation of RP-HPLC Method for Simultaneous Estimation of Triamterene and Benzthiazide in Tablets

  • Posted on: 1 June 2014
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PharmaTutor (June- 2014)
ISSN: 2347 - 7881

 

Received On: 17/04/2014; Accepted On: 22/04/2014; Published On: 01/06/2014

 

AUTHORS: VC Chauhan*, VN Shah, DA Shah, RR Parmar
Department of Quality Assurance
APMC College of Pharmaceutical Education and Research,
Motipura, Himmatnagar, Gujarat 383001
*vikas14vks@gmail.com

 

ABSTRACT:
A  specific,  accurate,  precise  and  reproducible  RP-HPLC  method  has  been  developed  and subsequently validated for the simultaneous determination of Triamterene and Benzthiazide in tablets. The proposed HPLC method utilizes BDS hypersil (Thermo scientific) C18 column (250 mm × 4.6 mm id, 5 μm particle size), and mobile phase consisting of phosphate buffer: methanol (70:30) and pH adjusted to 3.5 with sodium hydroxide and flow rate of 1.0 ml/min. Quantitation was achieved with UV detection at 245 nm based on peak area with linear calibration curves at concentration ranges 10-30 μg/ml for Triamterene and 5-15 μg/ml for Benzthiazide. The retention time of Triamterene and Benzthiazide were found to be 5.960 min and 3.493 min respectively.  The  method  was  validated  in  terms  of  accuracy,  precision,  linearity,  limits  of detection,  limits  of  quantitation  and  robustness.  This  method  has  been  successively  applied  to tablet formulation  and  no interference  from the  formulation excipients  was found.

How to cite this article: VC Chauhan, VN Shah, DA Shah, RR Parmar; Development and Validation of RP-HPLC Method for Simultaneous Estimation of Triamterene and Benzthiazide in Tablets; PharmaTutor; 2014; 2(6); 115-122

 

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