Chetana Ribadiya*1, Hemang Ribadia2, Nimish Talaviya3, Chandani Joshi3, Ashok Parmar3
1M.Pharm, Smt. R. B. Patel Mahila Pharmacy College, Atkot
2M.Pharm, Drug Regulatory Affairs Department of Pharmaceutical Science, Saurashtra University
3M.Pharm, Smt. R. D. Gardi B. Pharmacy College, Rajkot
A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Cefixime Trihydrate (CEF) and Linezolid (LNZ) by employing first order derivative zero crossing method in Methanol. The first order derivative absorption at 290 nm (zero cross point of CEF) was used for quantification of Linezolid and 228 nm (zero cross point of LNZ) for quantification of Cefixime Trihydrate. The linearity was established over the concentration range of 2-18 µg/ml and 7-15 µg/ml for Cefixime and Linezolid with correlation coefficient r2 0.9970 and 0.9982, respectively. The mean % recoveries were found to be in the range of 98.36 % – 99.45 % and 100.10 % – 101.66 % for Cefixime and Linezolid, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Cefixime and Linezolid in bulk and in market formulation.