Ganpat University

A REVIEW AND APPLICATION OF CRYOPROTECTANT: THE SCIENCE OF CRYONICS

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
Ankit J. Joshi
Department of Pharmaceutics & Pharmaceutical Technology,
S. K. Patel College of Pharmaceutical Education and Research,
Ganpat University, Ganpat vidyanagar, Kherva, Mehsana-Gozaria Highway, Gujarat.
Joshiankit2824@gmail.com

ABSTRACT
The preservation of cells, tissues and organs by cryopreservation is promising technology now days and Low temperature technology has progressed in the field of tissue engineering, food preservation, fertility preservation, making disease resistant breeds since the early years to occupy a central role in this technology. Cryopreservation is important technology in every field like in organ cryopreservation, food cryopreservation, human cryopreservation, seeds cryopreservation, protein cryopreservation and pharmaceuticals. Cryobiologists will be required to collaborate with new physical and molecular sciences to meet this challenge. How cryoprotectants work is a mystery to most people. In fact, how they work was even a mystery to science until just a few decades ago. This article will explain in basic terms how cryoprotectants protect cells from damage caused by ice crystals, and with some of the advances.

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CEFIXIME TRIHYDRATE AND LEVOFLOXACIN HEMIHYDRATE IN TABLET FORMULATION

ABOUT AUTHORS:
Sejal K. Patel, Sumeet I. Chhabra*
Department of Pharmaceutical Analysis,
Centre For Health Science Studies, Ganpat University,
Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India
*rxsumeet@gmail.com

ABSTRACT:
A simple, sensitive, accurate, precise and rapid reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of cefixime trihydrate and levofloxacin hemihydrate in tablet formulation. The chromatographic separation was performed on ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size). Mobile phase consisted of acetonitrile, water and methanol in the ratio of 65: 15: 25, v/v/v at a flow rate of 1.0 ml/min. The detection wavelength was set at 289 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The calibration was linear over the concentration range of 2-24 μg/ml for cefixime trihydrate and 2-30 μg/ml for levofloxacin hemihydrate. The retention times were found to be 1.9 ± 0.26 min for cefixime trihydrate and 3.6 ± 0.32 min for levofloxacin hemihydrate. The mean recoveries were 100.8 ± 0.54 and 100.1 ± 0.76 for cefixime trihydrate and levofloxacin hemihydrate, respectively. The method can be easily adopted for quality control analysis.

BRAIN TARGETING DRUG DELIVERY SYSTEM THROUGH NASAL CAVITY

ABOUT AUTHORS:
Bhavesh B. Patel*1, Nilam A. Patel2
1Department of Industrial Pharmacy
2Department of Pharmaceutics and Pharmaceutical technology
Shree S. K. Patel college of pharmaceutical education and research,
Ganpat University, Kherva, Mehsana – 384012, Gujarat.
*pbhavesh11@gmail.com

ABSTRACT
Nasal drug delivery has always been an area of research for industries because of its benefit in both local as well as systemic delivery of various therapeutic and debatably a drug delivery route to the brain. To use this route several parameters are being considered like the nature of disease condition (acute or chronic) and intended effects (local, systemic or central nervous system (CNS)) of drug treatment. Which formulation is better to target brain through nose would also be considered, device for effective nasal to brain drug delivery is also a develop thrust area for device companies. By nasal route BBB can be avoided, rapid action can be given in diseases like migraine, brain tumor, Alzheimer, Parkinson’s disease and hormone replacement. Direct delivery of intranasal drugs to the brain has been proposed, but is not universally established.

SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF MEROPENEM AND SULBACTAM SODIUM IN COMBINED DOSAGE FORM BY FIRST ORDER DERIVATIVE METHOD

ABOUT AUTHORS:
Patel Sannil R*, Patel Satish A
Department of Quality Assurance,
Shree S. K. Patel College of Pharmaceutical Education & Research,
Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
*srpatel2200@gmail.com

ABSTRACT
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical First order derivative spectrophotometry method for the simultaneous determination of Meropenem and Sulbactam Sodium in bulk and combined dosage form. The absorbance values at 333nm and 252 nm of first derivative spectrum was used for the estimation of Meropenem and Sulbactam Sodium, respectively without mutual interference. This method obeyed beer’s law in the concentration range of 5-70 μg/ml for Meropenem and 2-21 μg/ml for Meropenem. The method was successfully applied to pharmaceutical combined dosage form because no interference from the excipients was found. The suitability of this method for the quantitative determination of Meropenem and Sulbactam Sodiumwas proved by validation. The proposed method was found to be simple and sensitive for the routine quality control analysis of Meropenem and Sulbactam Sodium in bulk and combined dosage form. The results of analysis have been validated statistically and by recovery studies.

DEVELOPMENT AND VALIDATION OF FIRST ORDER DERIVATIVE METHOD FOR ANALYSIS OF EPERISONE HYDROCHLORIDE AND LORNOXICAM IN SYNTHETIC MIXTURE

ABOUT AUTHORS:
Harshil R. Patel*, Sejal K. Patel
Department of Quality Assurance,
S. K. Patel College of Pharmaceutical Education and Research,
Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
*harshil285@gmail.com

ABSTRACT
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical derivative spectroscopic methodfor the simultaneous determination of Eperisone Hydrochloride(EPE) and Lornoxicam (LOR) in synthetic mixture. Derivative spectroscopy offers a useful approach for the analysis of drugs in mixtures. In this study a first-derivative spectroscopic method was used for simultaneous determination of Eperisone Hydrochloride and Lornoxicam using the zero-crossing technique. The measurements were carried out at wavelengths of 264 nm and 225.2 nm for Eperisone Hydrochloride and Lornoxicam  respectively. The method was found to be linear (r2>0.998) in the range of 2- 30 μg/ml for Eperisone Hydrochloride at 264 nm. The linear correlation was obtained (r2>0.996) in the range of 2-14 μg/ml for Lornoxicam at 225.2 nm. The limit of detection was 0.2565 and 0.235 μg/ml for Eperisone Hydrochloride and Lornoxicam respectively. The limit of quantification was 0.7774 and 0.7121 μg/ml respectively. The method was successfully applied for simultaneous determination of Eperisone Hydrochloride and Lornoxicam in synthetic mixture.

miRNAs A NOVEL TARGET FOR ANTICANCER THERAPY

ABOUT AUTHORS:
Ketan M. Parmar*, Ritesh N. Sharma
S.K.Patel College of Pharmaceutical Education & research,
Department of Pharmaceutical chemistry, GANPAT UNIVERSITY.
*brave_student_90@yahoo.com

ABSTRACT
With the development of technologies to look at the expression levels of hundreds of miRNAs at a time and the clear role of miRNAs in cancers, groups began looking at miRNAs profiles of different cancers,especially the circulating miRNAs. We intended to make sure whether circulating miRNAs could be a promising biomarker of human cancers. Method: We comprehensively searched the Cochrane Library, Medline and EMbase from 1966 to Nov 2009 for the following terms: (“miRNA” or “microRNA”) and (“tumor” or “carcinoma”) and (“plasma” or “serum” or “circulating”). Detailed information was extracted from studies that met the inclusion criteria: blood-based miRNAs in human cancers and studies published in the English literature. Results: The current review show that different researches use different measurement methods which might impact the results;Cancers treatment might have an effect on circulating miRNAs; some miRNAs are multi-faceted RNA; small sample size might produce selection bias. Furthermore, because of the lack of randomized controlled trials and the heterogeneous nature of the available data, no attempt was made to perform quantitativemeta-analyses.
In this review, based on those researches, circulating miRNAs are promising and difficulties for their future application for diagnosing human cancers.

RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF MONTELUKAST SODIUM AND DESLORATADINE IN COMBINED DOSAGE FORM

ABOUT AUTHORS:
Rima M. Bankar*, Dipti B. Patel
Department of Pharmaceutical Analysis,
Shree S. K. Patel College of Pharmaceutical Education & Research, Ganpat University,
Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
*rima.banker@yahoo.com

ABSTRACT
A novel, precise, accurate and rapid isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for  simultaneous determination of Montelukast Sodium and Desloratadine. The method showed adequate separation for Montelukast Sodium and Desloratadine  and best resolution was achieved with ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size) using Acetonitrile-Methanol-Water (15:80:5, v/v) as a mobile phase at a flow rate of 1.0 ml/min and wavelength of 283 nm. The calibration curves were linear over the concentration ranges of 5-50 μg/ml for Montelukast Sodium and Desloratadine. The limit of detection (LOD) and limit of quantification (LOQ) for Montelukast Sodium were 0.33 and 1.01 μg/ml while for Desloratadine were 0.10 and 0.31 μg/ml, respectively. All the analytes were separated in less than 6.0 min. The proposed method could be applied for routinelaboratory analysis of Montelukast Sodium and Desloratadine in pharmaceutical dosage form. Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in synthetic mixture of both drugs with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.


SIMULTANEOUS ESTIMATION OF EPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM BY RATIO SPECTRA DERIVATIVE SPECTROPHOTOMETRY METHOD IN SYNTHETIC MIXTURE

ABOUT AUTHORS:
Rinku B Patel*1, Paresh U Patel2, Bharat G Patel3, Anil C Patel2
1Department of Pharmaceutical Analysis, Centre For Health Science Studies, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
2Department of Quality Assurance, Centre For Health Science Studies, Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
3Aspee college of Home Science and Nutrition, S.D.Agricultural University, S.K.Nagar-385506, Banaskantha, Gujarat, India.

*rinkupatel5890@gmail.com

ABSTRACT
Simple, accurate, precise, and sensitive ratio spectra derivative spectrophotometric method for simultaneous estimation of Eperisone hydrochloride (EPE) and Diclofenac sodium(DIC) in synthetic mixture have been developed and validated. The ratio derivative spectroscopic method involves measurement of first derivative amplitude of ratio spectra at 247 nm for EPE and 218.4 nm for DIC as two wavelengths for estimation. Beer's law is obeyed in the concentration range of 2-18 μg/ml for both EPE and DIC. LOD values for EPE and DIC are found to be 0.0634 μg/ml and 0.5386 μg/ml, respectively. LOQ values for EPE and DIC are found to be 0.1921 μg/ml and 1.6321 μg/ml, respectively. The method was validated statistically and recovery studies were carried out. It was found to be accurate, precise and reproducible. The method was applied to the assay of the drugs in synthetic mixture, which were found in the range of 98.0% to 102.0% of the labeled value for both Eperisone hydrochloride and Diclofenac sodium. Hence, the method herein described can be successfully applied in quality control of synthetic mixture.

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Ibuprofen and Chlorzoxazone in Synthetic Mixture

ABOUT AUTHORS
Anil C. Patel*, Dr Paresh U. Patel , Rinku B. Patel
Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research,
Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
*anilpatel002@gmail.com

ABSTRACT
A novel, precise, accurate and rapid isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for  simultaneous determination of Ibuprofen and Chlorzoxazone. The method showed adequate separation for Ibuprofen and Chlorzoxazone and best resolution was achieved with ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size) using Acetonitrile-Phosphate buffer pH 3.5 - Methenol (20:20:60, v/v; pH adjusted to 3.5 with O-phosphoric acid and TEA(Tetra ethyl amine) as a mobile phase at a flow rate of 0.7 ml/min and wavelength of 221 nm. The calibration curves were linear over the concentration ranges of 2-30 μg/ml for Ibuprofen and Chlorzoxazone. The limit of detection (LOD) and limit of quantification (LOQ) for Ibuprofen were 0.96 and 2.92 μg/ml while for Chlorzoxazone were 0.69 and 2.09 μg/ml, respectively. All the analytes were separated in less than 6.0 min. The proposed method could be applied for routinelaboratory analysis of Ibuprofen and Chlorzoxazone in pharmaceutical dosage form. Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in synthetic mixture of both drugs with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF TOLPERISONE HYDROCHLORIDE AND DICLOFENAC SODIUM IN SYNTHETIC MIXTURE

ABOUT AUTHORS
Satish A. Patel, Kaushik P Hariyani*
Department of Quality Assurance, S. K. Patel College of Pharmaceutical Education and Research,
Ganpat University, Ganpat Vidyanagar – 384012, Mehsana, Gujarat, India.
*hariyanikaushik@gmail.com

ABSTRACT
A simple, sensitive, accurate, precise and rapid reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Tolperisone hydrochloride and Diclofenac sodium from synthetic mixture. The chromatographic separation was performed on ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size). Mobile phase consisted of a mixture of phosphate buffer pH 6, acetonitrile and methanol in the ratio of 10: 50: 40, v/v/v at a flow rate of 0.7 ml/min. The detection wavelength was set at 267 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The calibration was linear over the concentration range of 2-30 μg/ml for Tolperisone hydrochloride and 2-30 μg/ml for Diclofenac sodium. The retention times were found to be 2.1 ± 0.14min for Diclofenac sodium and 4.7 ± 0.13min for Tolperisone hydrochloride. The mean recoveries were 100.5 ± 0.34 and 100.8 ± 0.80 for Tolperisone hydrochloride and Diclofenac sodium, respectively. The method can be easily adopted for quality control analysis.

Pages