• PharmaTutor
    3 January 2013

    EFFECT OF LYOPHILIZATION AND CRYOPRESERVATION ON PLANT LEAVES OF TERMINALIA ARJUNA TERMINALIA CATAPPA, TERMINALIA CHEBULA, JATROPHA GOSSYPIFOLIA, JATROPHA CURCAS

    About Authors:
    1Hardik R. Patel*, 2Upanita C. Patel
    1Industrial biotechnologist, 2Microbiologist
    Anand, Gujarat, India.
    *hardikigbt@gmail.com

    INTRODUCTION:
    Cryopreservation and lyophilization of plant germplasm has obvious advantages over in vitro storage in term of space saving and improved phytosanitation. We compared cryopreserved and lyophilized leaf as sources for genomic DNA isolation by CTAB protocol and PVP protocol.Our results showed that cryopreservation of leaf tissue yielded high molecular weight genomic DNA. The DNA was suitable for restriction-enzyme digestion and as a template for polymerase chain reaction (PCR) amplification. While these results rule out cryopreserved  tissue as a source for DNA isolation, the ability to freeze-dry, powder, and efficiently store voluminous tissue samples for later use in DNA and protein isolation could be of great benefit to laboratories involved in  molecular genetics and molecular biology.

  • 2 January 2013
    Multiple vacancies for B.Pharm, M.Pharm, M.Sc, D.Pharm in Production, QC/QA, Technicians @ Alembic Pharma

    Alembic Limited, with an established presence in the Indian pharma  industry has a proud, historical track record going back one hundred years. With a turnover in excess of Rs1000 crores today, Alembic Limited is one of India’s leading integrated pharmaceutical companies.

  • 31 December 2012
    Indian Institute of Advanced Research invites Junior Research Fellow and Lab Assistant

    Applications are invited for the position of JRF & Lab attendant in GSBTM, Govt. of Gujarat sponsored project: "To study the role of ubiquitin proteosome system and molecular chaperone 70 in the progression of Alzheimer's disease using Drosophila as a model organism."

    Post: Junior Research Fellow and Lab Assistant

  • 26 December 2012
    Application are invited for the post of Junior Research Fellow in INDIAN INSTITUTE OF ADVANCED RESEARCH(IIAR)

    Applications are invited for the position of Junior Research Fellow (JRF) in DBT sponsored project "Unravelling the cross talk between inflammation and apoptosis: Focusing on regulation of inflammatory mediators and apoptosis in cancer".

    Post: Junior Research Fellow

  • 26 December 2012
    Franco-Indian Pharmaceuticals invites Regional Sales Managers, Area Sales Managers, Trainee Medical Representatives

    Franco-Indian Pharmaceuticals Pvt. Ltd., a name that stands for quality and innovation, is one of the major players in the Pharmaceutical Industry in India.
    Our innovative, value added products improve the quality of life of people and ease their sufferings and help them to enjoy, longer, healthier and more productive lives.

  • 19 December 2012
    EFFECT OF CRYOPRESERVATION, LYOPHILIZATION ON DNA EXTRACTION PROTOCOL OF ACACIA ARABICA, ACACIA SINUATA, PROSOPIS SPICIGERA, ADENANTHERA PAVONINA AND ACACIA AURICULIFORMIS

    About Authors:
    Hardik R. Patel
    Industrial Biotechnology from Sardar Patel University,
    Gujarat, India.
    hardikigbt@gmail.com

    ABSTRACT
    Cryopreservation and lyophilization of plant germplasm has obvious advantages over in vitro storage in term of space saving and improved phytosanitation. We compared cryopreserved and lyophilized leaf as sources for genomic DNA isolation by CTAB protocol and PVP protocol.Our results showed that cryopreservation of leaf tissue yielded high molecular weight genomic DNA. The DNA was suitable for restriction-enzyme digestion and as a template for polymerase chain reaction (PCR) amplification. While these results rule out cryopreserved  tissue as a source for DNA isolation, the ability to freeze-dry, powder, and efficiently store voluminous tissue samples for later use in DNA and protein isolation could be of great benefit to laboratories involved in  molecular genetics and molecular biology.

  • 18 December 2012
    CSIR Sponsored Workshop on Hands on training on software related to Computer Aided Drug Design at Nirma University

    Nirma University (NU) was established in the year 2003 as a Statutory University under a Special Act passed by the Gujarat State Legislative Assembly. The University Grants Commission (UGC) has recognized Nirma University under Section 2(f) and the University is also accredited by National Assessment and Accreditation Council (NAAC). Nirma University is a member of the Association of Indian Universities (AIU) and the Association of Commonwealth Universities (ACU).

  • 9 December 2012
    Medical and Health Services, Dadra and Nagar Haveli invites applications for Pharmacist

    Medical and Health Services, Dadra and Nagar Haveli invites applications for the following post on regular basis.

    Post: Pharmacist Group C

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  • 27 November 2012
    Oil And Natural Gas Corporation invites Pharmacist GD.III (Allopathy) | 10 Posts

    ONGC, a ‘Maha Ratna’ Public Sector Enterprise, and India’s flagship energy company is engaged in Exploration and Production of Oil and Gas in India and abroad. A Global Energy majors, it contributes to 80% of India’s Domestic Oil Production. ONGC’s annual net profit is excess of Rs. 25,123 Crores currently. ONGC, through its subsidiary ONGC Videsh Ltd.(OVL), is India’s biggest Transnational Corporate with overseas investment of over 10 Billion USD in 16 Countries.

  • 25 November 2012
    Rolling advertisement for recruitement of Professor, Assistant Professor, Associate Professor in Gujarat Technological University

    The Gujarat Technological University was established vide Gujarat Act No. 20 of 2007. The main objective of the University is to develop the knowledge of science, engineering, technology, management and environment for the advancement of quality of life of the mankind in general. The prime objects of the University shall be to create centres and institutes of excellence is basic and applied science, engineering and technology.

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