RP-HPLC METHOD FOR THE ESTIMATION OF NITAOXANIDE IN PHARMACEUTICAL FORMULATION
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ABOUT AUTHORS:
R.Meera1*, N.Swathylakshmi2, M.Sundarapandian2, P.Raja Soundara Pandian1, Madhavanmallayasamy1
1Researcher, Radianz Health Care Pvt Ltd, Madurai, Tamilnadu, India
2Department of pharmaceutical Chemistry, K.M.College of pharmacy, Uthangudi, Madurai, India
meeraharsa23@gmail.com
ABSTRACT
Objective: A simple and precise RP-HPLC method was developed and validated for the determination of Nitaoxanide in pharmaceutical dosage forms.
Materials and Methods: Chromatography was carried out using waters RP –C18 150×4.6 mm, 3.5 µ, pH 6.8, buffer: acetonitrile (50:50) as the mobile phase at a flow rate 1.2 ml/min. The analyze was monitored using PDA detector at 254 nm. The proposed method was found to have linearity in the concentration range of 25-150µg/ml with correlation co efficient of r2 =0.9999.
Results:The developed method has been statistically validated and found simple and accurate. The mean recoveries obtained for Nitaoxanide were in the range 100.06-101.9%.
Conclusion:Due to its simplicity, rapidness, high precision and accuracy of the proposed method it may be used for determining Nitaoxanide in bulk and dosage forms.


