EXTRACTION AND PURIFICATION OF NUCLEIC ACID USING CBNP & PCIA TECHNIQUE
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About Author
Nirav Soni
Department of Quality Assurance,
A-One Pharmacy College,
Anasan, Ahmedabad, Gujarat, India
nirav_sonic@yahoo.com
ABSTRACT
Isolation of Nucleic acid easily by Solid Phase Extraction (SPE) and this approach, using commercially available Extraction of nucleic acid column-based kits, requires no toxic chemicals and is a rapid and consistent method for concomitant protein extraction. It is a modern technique useful for separation of Nucleic acid which is most reliable, less time consuming and separation of impurities and continuity of reactive products using listed techniques like column-based nucleic acid purification, Nucleic acid methods& ethanol precipitation, DNA separation by silica adsorption. Buffer choice is significant to completely solubilized all proteins in the sample. This technique provides a simple and effective way to analyze protein and nucleic acids simultaneously from the same sample not affecting yield and quality.
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