• PharmaTutor
    21 September 2012

    BIOSENSOR - Micro electrochemical device

    About Authors:
    Vedant M. Pandya
    Department of biotechnology, shree M & N Virani science college,
    Rajkot, India-360005
    vedantpandya007@gmail.com

    ABSTRACT
    A biosensor is a device for the detection of an analyte that combines a biological component with physicochemical detector components. An integrated device consisting of a biological recognition element & a transducer capable of detecting the biological reaction & converting it into a signal which can be processed. Ideally, the sensor shod be self-contained, so that it is not necessary to add reagents to the sample matrix to obtain the desired response. There are a number of analytes which are measured in biological media: pH, partial pressure of carbon dioxide, partial pressure of oxygen & the ionic concentration of sodium, potassium, calcium & chloride. Biosensors are typically classified by the type of recognition element or transduction element employed. Sensors might be described as a catalytic biosensor if it is recognition element comprised an enzyme or series of enzymes, a living tissue slice, or whole cells derived from microorganisms such as bacteria, fungi, or yeast. The sensor might be described as a bioaffinity sensor if the basis of operation were a biospecific complex formation. Accordingly, the reaction of an antibody with an antigen or hapten, or the reaction of an ageist or antagonist with a receptor, could be employed. In the former case, the sensor might be called an immunosensor. Since, enzyme-based sensor measure the rate of the enzyme-catalyzed reaction as the basis for their response, any physical measurement which yield a quantity related to this rate can be used for detection. The enzyme may be immobilized on the end of an optical fiber, & the spectroscopic properties related to the disappearance of the reactants or appearance of products of the reaction can be measured. Since biochemical reaction can be either endothermic or exothermic. Miniaturized thermistor based calorimeter called thermistos, have been developed & widely applied, especially for bioprocess monitoring.

  • 17 September 2012
    RECENT ADVANCES OF LACCASE ENZYME IN INDUSTRIAL BIOTECHNOLOGY : A REVIEW

    About Authors:
    Alpeshkumar J.Shiroya*
    Bhagwan Mahavir College Of  Biotechnology,
    Surat
    alpeshshiroya45@yahoo.in

    ABSTRACT
    Laccases are an interesting group of multi-nuclear copper-containing oxidoreductases, which have been subject of intensive research in last decades due to their ability to oxidize both phenolic and nonphenolic lignin related compounds as well as highly recalcitrant environmental pollutants, which makes them very useful for their application to several biotechnological processes. These oxidase enzymes catalyze oxidation of substrates at the expense of molecular oxygen and produce water as the only by-product. Lacasses are present in higher plants, bacteria, fungi, insects and lichens. The oxidative ability of laccases is employed in a number of industrial and environmental applications including bioremediation, food technology, nanobiotechnology, medicine, pulp and paper industry, textile industry, and cosmetology. Owing to its versatility, laccase is continuously under investigation for new applications. In the recent years, laccases are also used as cleaning agents for certain water purification systems, as catalysts for the manufacture of anti-cancer drugs and even as ingredients in cosmetics. More recently, it is also used in the design of biosensors, biofuel cells, as a medical diagnostics tool and bioremediation agent to clean up herbicides, pesticides and certain explosives in soil. This paper reviews the occurrence, mode of action, general properties, production, immobilization, molecular cloning, and important  application  of laccases  within different industrial and biotechnological area.

  • 16 September 2012
    Walk in interview @ Zydus Cadila for experience candidates in API as Chemists/Sr. Chemists/Officers - R&D, Production, Quality Control - 10 posts

  • 11 September 2012
    Application are invited for the post of Senior Research Fellow in Indian Institute of Advanced Research

    Applications are invited for the position of SRF in ICMR-sponsored project: “Study of Genetic Factors Governing Multidrug Resistance in Clinical Isolates of Shigella spp.” with Dr. Ashima Bhardwaj, Assistant Professor, Department of Human Health and Diseases, IIAR.

    Position : SRF (One post)

  • 4 September 2012
    Zydus Cadila invites Chemists/Sr. Chemists/Officers Production, Chemists/Sr. Chemists/Officers: - Quality Control, Office/Executive - TT (Technology Transfer) - Baroda

  • 29 August 2012
    DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR ESTIMATION OF TADALAFIL AND DAPOXETINE HCL IN SOLID DOSAGE FORM

    About Authors:
    CHIRAG S. RAJPARA*1, JAWED AKHTAR1, AMIT KHANDHAR2
    1. Department of Quality Assurance, School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur-302025, Rajasthan, India.
    2. Zydus cadila pharma, moraiya,ahmedabad.
    *chiragrajpara2601@gmail.com

    ABSTRACT
    * A simple, specific, accurate and stability-indicating reversed phase high performance liquid chromatographic method was developed for simultaneous estimation of Tadalafil and Dapoxetine HCL, Water Symmetry C-18 (150 x 4.6 mm),5 µ and a mobile phase composed of Buffer : Acetonitrile (65:35)

    * The retention time of Tadalafil and Dapoxetine HCL were found to be10.08 and 4.45 min respectively. Linearity was established for Tadalafil and Dapoxetine HCL in the range of 8.0-60 μg/ml and 24.0-180.0 μg/ml respectively. The percentage recovery of Tadalafil and Dapoxetine HCL were found to be in the range of 99.7-100.6% and 98.07-99.09% respectively. The drug was subjected to acid and alkali hydrolysis, oxidation, dry heat and photolytic degradation. The degradation studies indicated, Tadalafil showed degradation in acid and alkali while it was found stable in H2O2, photolytic and in presence of dry heat and Dapoxetine showed degradation in thermal and peroxide while it was found stable in rest of parameters . The degradation products of Tadalafil and Dapoxetine HCL in acidic, alkaline conditions were well resolved from the pure drug with significant differences in their retention time values. This method can be successfully employed for the quantitative analysis of Tadalafil and Dapoxetine HCL in bulk drugs and formulations.

  • 28 August 2012
    SYNTHESIS AND BIOLOGICAL EVALUATION OF SOME NOVEL HETEROCYCLES DERIVED FROM 2-ARYLIDENE-1-TETRALONES

    About Authors:
    Roshni Patel*, Prof. Arun Parikh, Prof. Vijayalakshmi  Gudaparthi
    Department of Pharmaceutical Chemistry, L.J.Institute of Pharmacy,
    S.G.Highway, Ahmedabad-382210
    *roshanimpharm@gmail.com

    ABSTRACT
    Medicinal chemistry involves chemical aspects of identification, and then systematic, thorough synthetic alteration of new chemical entities to make them suitable for therapeutic use. Indazoles have an important role in medicinal chemistry. Indazole  nucleus is an important class of nitrogen containing heterocyclic widely used as key building block for the synthesis of various pharmaceutically important agents. Indazole possess wide range of biological activities such as bactericidal, fungicidal, analgesic, antihypertensive, anti-inflammatory and antitumor. In the present study we have synthesized some novel 3,3a,4,5-tetrahydro-2-(substituted benzenesulphonyl)-3-(substituted phenyl)-2H-benzo[g]indazoles, by condensation of  1-tetralone with different substituted aromatic aldehydes and the resulted 2-(substituted benzylidene)-3,4,-dihydronaphtalen-1-ones on reaction with hydrazine hydrate in methanol followed by treatment with different substituted sulfonyl chlorides in pyridine gave the titled compounds.The synthesized compounds were characterized by IR, NMR and Mass spectral analysis. All newly synthesized derivatives were evaluated for antibacterial activity against  gram + ve and gram-ve bacteria B.cereus, S.aureus and E.coli respectivelyand antifungal activity againstC.albicans and all the synthesized compounds showed significant antibacterial and antifungal activity.

    [adsense:336x280:8701650588]

  • 26 August 2012
    J. B. Chemicals & Pharmaceuticals invites for high profile QC Manager

    J. B. Chemicals & Pharmaceuticals Ltd. (JBCPL), known to many as Unique Pharmaceutical Laboratories is one of India's leading global pharmaceutical companies. The company manufactures and markets a wide range of pharmaceutical formulations.

  • 25 August 2012
    SIMULTANEOUS ESTIMATION OF FUROSEMIDE AND SPIRONOLACTONE IN COMBINED PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

    About Author:
    Hardik Patel*, Sagar Solanki
    Department of Pharmaceutical Chemistry,
    K.B.Raval College of Pharmacy, Shertha,
    Gandhinagar-382423, Gujarat, India.
    *patel1928@yahoo.in

    ABSTRACT
    A new, simple, rapid, accurate, precise and sensitive method has been developed for the simultaneous estimation of Furosemide and Spironolactone in their combined tablet dosage form. The method was carried out on a Hiber C18 column (250 mm×4.6mm, i.d.5 μm) with a mobile phase consisting of acetonitrile: water at a flow rate of 1 ml/min and the detection was carried out at 237 nm. The retention time of Furosemide and Spironolactone was 3.81 min and 7.28 min. respectively. Linearity for Furosemide and Spironolactone were found in the range of 2-10 μg/ml and 5-25 μg/ml respectively. The developed method was validated in terms of linearity, accuracy, and precision, limit of detection (LOD) and limit of quantification (LOQ). The proposed method can be used for estimation of both drugs in their combined dosage form.

  • 24 August 2012
    Walk in Interview for post of Pharmacists in Western Railways - 8 posts | Freshers

    It is proposed to engage full time Para Medical staff for the following posts purely on contract basis on BCT Division of Western Railway. The details of the post, place of posting, remuneration etc. and other terms and conditions stipulated are given below:-

Subscribe to Gujarat