About Authors:
Nitin Deorao Rewatkar
Department of Biotechnology, Kamla Nehru College,
Sakkardara Square, Nagpur
nitinrewatkar@gmail.com
Abstract
The effect of TDZ on regeneration studies of cotton G.arboreum cv PA255 was performed for in-vitro culture studies, healthy and disease free seeds, after surface sterilization were inoculated on seed germination media. Seeds germination efficiency was observed to be 73.38%. Shoot tips and cotynode explants aseptically isolated from in Vitro germinated seedlings of 7 and 14 days old were inoculated on MS basal salt supplemented with 30 gm/L glucose, 10 mg/L thiamine, 100 mg/L Inositol with four different concentration of TDZ. The proliferation of 7 and 14 days old shoot tip was found to 100% in media combination with 0.08 TDZ and 0.02 TDZ respectively. Cotynode explants of 7 days old was 100% responded in 0.02 TDZ and 0.08 TDZ, 14 days old cotynode shows highest 85% explants responded in 0.02 TDZ media combination but multiple shoots observed higher in 0.05 TDZ medium.TDZ induces callus formation and inhibit root formation, TDZ shows good responsein very small concentration otherwise increase concentration more than 1 mg/l it shows toxic effect on explants and completely dried. Browning and subsequent death of the cultured explants are major problems in TDZ supplemented media. Calculate the amount of phenol excreted from shoot tip and cotynode during in vitro regeneration of cotton cv. PA255. In the media combination 0.1mg /L TDZ evaluate highest phenol secreted, shoot tip secrete average 0.014mg and cotynode secrete 0.022mg phenol by per explants.
