Anand

EFFECT OF LYOPHILIZATION AND CRYOPRESERVATION ON PLANT LEAVES OF TERMINALIA ARJUNA TERMINALIA CATAPPA, TERMINALIA CHEBULA, JATROPHA GOSSYPIFOLIA, JATROPHA CURCAS

About Authors:
1Hardik R. Patel*, 2Upanita C. Patel
1Industrial biotechnologist, 2Microbiologist
Anand, Gujarat, India.
*hardikigbt@gmail.com

INTRODUCTION:
Cryopreservation and lyophilization of plant germplasm has obvious advantages over in vitro storage in term of space saving and improved phytosanitation. We compared cryopreserved and lyophilized leaf as sources for genomic DNA isolation by CTAB protocol and PVP protocol.Our results showed that cryopreservation of leaf tissue yielded high molecular weight genomic DNA. The DNA was suitable for restriction-enzyme digestion and as a template for polymerase chain reaction (PCR) amplification. While these results rule out cryopreserved  tissue as a source for DNA isolation, the ability to freeze-dry, powder, and efficiently store voluminous tissue samples for later use in DNA and protein isolation could be of great benefit to laboratories involved in  molecular genetics and molecular biology.

FORMULATION AND EVALUATION OF AMOXICILLIN TRIHYDRATE MODIFIED RELEASE DOSAGE FORMS

About Author:
*1 Patel Dimpalben Girishkumar, 2 Mr.K.H.Shah, 3 Rohit K Patel,
3Yatish Shukla, 3 Modi B, 3 Nilesh Patel
1 Dharmaj Degree Pharmacy College,

Dist- Anand, Dharmaj -388430, Gujarat
2 Professor, IPCPRC, Dharmaj, Gujarat
3 KAPTAB Pharmaceuticals
*dimplepatel70@gmail.com

Abstract
The aim of the current investigation is to design oral once daily modified  release dosage forms of amoxicillin trihydrate for treatment of pharyngitis/tonsilitis,  which release the drug for 24 hours and match with theoretical drug release profile.  The tablets and capsules were prepared by the different method using different  polymers in different concentrations. The interference of the polymers was ruled out  by FT-IR spectroscopy studies. The powder-blends of tablets and drug were evaluated  for their physical properties like angle of repose, bulk density, compressibility  index, and Hausner ratio and found to be satisfactory. The manufactured tablets  were evaluated for in process and finished product quality control tests including  appearance, thickness, weight variation, hardness, friability, drug content, and in  vitro drug release. All formulations showed appearance, thickness, weight variation,  hardness, friability and drug content in specified limit. All formulations showed  acceptable pharmacotechnical properties and complied with in-house specifications  for tested parameters. The results of dissolution studies indicated that formulation  containing 50% ethyl cellulose and 50% methocel was the most successful  formulation which was evidenced by similarity (f2) and dissimilarity (f1) factors. The  formulated amoxicillin trihydrate tablets followed zero order release kinetics and  Higuchi diffusion was the dominant mechanism of drug release, resulting in regulated  and complete release within 24 hours. Formulations were subjected to short term  stability studies as per ICH guidelines and were found stable. Capsule formulations  16  were evaluated for weight uniformity, drug content and in vitro drug release. The  results of dissolution studies indicated that drug release from capsule not extend up to  24hrs. All formulations of capsule failed in in-vitro drug release test. In comparison of  tablet and capsule formulations, tablet found to be successful dosage form.

Simultaneous Estimation of Tramadol HCl, Paracetamol and Domperidone in Pharmaceutical Formulation by Thin-Layer Chromatographic-Densitometric method

About Authors:
Keyur B.ahir, Emanual M. Patelia*, Falgun A.Mehta
Department of Pharmaceutical Chemistry and Analysis,
Indukaka Ipcowala College of Pharmacy,
New Vallabh Vidyanagar – 388121, Gujarat, India
*ricky.emanual@gmail.com

Abstract:
A simple, precise, rapid, selective, and economic high-performance-thin-layer chromatography (HPTLC) method has been established for simultaneous analysis of Domperidone (DMP), Paracetamol (PCM) and Tramadol Hcl (TMD) in tablet dosage forms. The chromatographic separations were performed on precoated silica gel 60254 plates with toluene-ethylacetate-butanol-ammonia 5:4:1:0.2(v/v) as mobile phase. The plates were developed in a 7.0 cm at ambient temperature. The developed plates were scanned and quantified at their single wavelength of maximum absorption at approximately 278 nm for DMP and PCM, respectively. Experimental conditions such as chamber size, chamber saturation time, migration of solvent front, slit width, etc. were critically studied and the optimum conditions were selected. The drugs were satisfactorily resolved with Rf 0.18 ± 0.02 for DMP, Rf 0.25 ± 0.02 for PCM and for TMD Rf 0.50 ± 0.02. The method was validated for linearity, accuracy, precision, and specificity. The calibration plot was linear between 100-600 ng / band for DMP, 3250-19500 ng / band based for PCM and 375-2250 ng / band based for TMD. The limits of detection and quantification for DMP were 9.95 and 30.16ng / band, respectively; for PCM they were 64.30 ng and 194.87 ng / band and for TMD 5.51 and 16.70/ band. This HPTLC procedure is economic, sensitive, and less time consuming than other chromatographic procedures. It is a user-friendly and importance tool for analysis of combined tablet dosage forms.

Synthesis and Antimicrobial activity of Bulky molecules comprising Benzothiazole and Sulphonamide moieties

About Authors:
Pritesh R. Patel*, Priyank Patel, Jagath Pillai, Nilesh Darji, Bhagirath Patel
Department of Pharmaceutical Chemistry,
Sat Kaival College of Pharmacy, Sarsa,
Ta & Dist: Anand (Gujarat), India-388365

*prit.pharma@gmail.com

ABSTRACT
As part of ongoing studies in developing new antimicrobials, a novel series of 4-acetamido-N-(substituted 1, 3-benzothiazol-2-yl) benzenesulphonamide and N-(substituted 1, 3-benzothiazol-2-yl)-4-(substituted aryl diazenyl) benzenesulphonamide were synthesized in order to determine their antibacterial and antifungal activity. The synthesized compounds were tested in vitro against two Gram-positive bacteria like Staphylococcus aureus, Bacillus subtilis; two Gram-negative bacteria like Escherichia coli, Pseudomonas aeruginosaand one fungal strain Candida albicans in comparison with standard drugs. Microbiological results showed that the synthesized compounds possessed a broad spectrum of antibacterial and antifungal activity against the tested microorganisms. The compounds with a 6-chloro (SK5b), 7-chloro-6-fluoro(SK5d) and 6-nitro (SK5e) on 2-amino benzothiazole ring possessing azo linkage showed better antimicrobial activity; almost similar or less to that of standard drugs thus they could be promising candidates for novel drugs. The novel heterocyclic derivatives were characterized by Physical characterization (Melting point, TLC) and different Spectroscopy techniques (IR, 1H NMR and Mass spectroscopy).

Faculty vacancies in A.R.College of Pharmacy & G.H.Patel Institute of Pharmacy AND Indukaka Ipcowala College of Pharmacy - 16 vacancies

Set up in 1981 with generous donation from Shrimati Anjanaben Ashokbhai Shah and Shri Gordhanbhai Hathibhai Patel the institutions offer the following courses:
D.Pharm, B.Pharm, M.Pharm (Eligibility for admission is minimum of 60% marks in B. Pharm.) (Eligibility for admission is M. Pharm.) Main areas of pharmaceutical sciences covered are Pharmaceutics and Pharmaceutical Technology, Pharmaceutical Chemistry & Pharmaceutical Analysis, Pharmacology & Toxicology and Pharmacognosy & Phytopharmaceuticals.

Multiplace Vacancies in Unichem for the post of Business Executive - Gynec Division | all India level

Unichem Laboratories has grown to become one of India’s most respected pharmaceutical companies. It is committed to delivering better health through superior products. By combining strategic research and in-depth industry knowledge, Unichem aims to transform itself into a global pharmaceutical drug company with an increasing focus on cutting-edge research and developed markets.

Teaching & nonteaching staff require in Sardar Patel College of Pharmacy | Principal, Professors, Assistant professors & Lecturers (in various dept.)

Sardar Patel University comprises of 25 Postgraduate Departments, a constituent collage, and number of colleges affiliated to it. There are 14 PG Centers in the affiliated Colleges, linstitutions. The teaching programme covered at Postgraduate, Medicine, Homeopathy, Home Science, Law and Education includes Postgraduate Degree Courses, Postgraduate Diploma Courses, Diploma Courses, and Advanced Certificate Courses, Undergraduate Courses are taught in the colleges or institutes affiliated to the University.

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