Zero order and First order derivative spectroscopic method for simultaneous estimation of Moxifloxacin hydrochloride and Ketorolac tromethamine in simulated tear fluid

  • Posted on: 31 March 2016
  • By: admin

 

 

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PharmaTutor (April- 2016)

 

Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 4, Issue 4)

 

Received On: 01/11/2015; Accepted On: 07/11/2015; Published On: 01/04/2016

 

AUTHORS:
Shashank Nayak N*1, U Srinivasa2, Shwetha S Kamath K3, Shabaraya AR
1Dept of Pharmaceutics, Pacific university, Udaipur, Rajasthan, India
2Dept.of Pharmacognosy, Srinivas college of pharmacy, Mangalore, Karnataka, India
3Dept. of Pharmaceutics, Srinivas college of pharmacy, Mangalore, Karnataka, India
*shashanknayak87@gmail.com

 

ABSTRACT: In this current work simultaneous estimation of Moxifloxacin HCL and Ketorolac tromethamine in simulated tear fluid was performed by using UV spectroscopy. The zero order derivative spectroscopy revealed that Moxifloxacin HCL had a λ max of 288 nm in Simulated tear fluid(STF) and Ketotorolac tromethamine had λmax of 322nm respectively. The linearity was found in the range of 2-10 μg/ml for Moxifloxacin HCL and 2-14 μg/ml for ketorolac tromethamine. The R2 value was found to be 0.998 and slope y=0.089 for Moxifloxacin HCL and R2 =0.998 and slope y=0.051 for Ketorolac tromethamine. The zero order spectrum was deravatized to first order derivative spectra for both the above mentioned drug. The first order derivative spectra of Moxifloxacin HCL and ketorolac tromethamine indicated that it had a zero crossing point (ZCP) at 286 nm and 328 nm. The absorbance of the mixture containing MOXI and KETO was found out at 286 nm and 328 nm which showed linearity and obeyed beers lamberts law.

 

 

How to cite this article: Shashank NN, Srinivasa U, Kamath SS, Shabaraya AR; Zero order and First order derivative spectroscopic method for simultaneous estimation of Moxifloxacin hydrochloride and Ketorolac tromethamine in simulated tear fluid; PharmaTutor; 2016; 4(4); 43-47

 

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