Guntur

ENHANCEMENT OF SOLUBILITY; AN OVERVIEW

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
Ramesh Babu Pedada1*, Eukondalu Vanka1, Dr.A.M.S.Sudhakar Babu1, Prasanna kumar Desu1, P.Ramaa Bharathi1, P.Venkateawara.rao2
1Department of Pharmaceutics, 2Department of Pharmaceutical  Analysis,
A.M.Reddy Memorial College of pharmacy, Narasaraopet, Guntur (Dt), Andhra Pradesh, India.
Rameshbabu.pedada@gmail.com

ABSTRACT:
Enhancement of solubility, dissolution rate and bioavailability of drug is a very challenging task in drug development, nearly 40% of the new chemical entities currently being discovered are poorly water soluble drugs. Aqueous solubility of any therapeutically active substance is a key property as it governs dissolution, absorption and thus the in vivo efficacy. Orally administered drugs completely absorb only when they show fair solubility in gastric medium and such drugs shows good bioavailability. The solubility and dissolution properties of drugs play an important role in the process of formulation development. Problem of solubility is a major challenge for formulation scientist which can be solved by different technological approaches during the pharmaceutical product development work. The present review deals in detail about the solubilisation by surfactants, cosolvents, complexation for the improvement of solubility of poorly water soluble drugs.

AN OVERVIEW ON PULSATILE DRUG DELIVERY SYSTEM

{ DOWNLOAD AS PDF }

ABOUT AUTHORS:
Edukondalu.Vanka*, M. Jhansi Rani, A.M.Sudhakar Babu, P.Venkatesawara Rao
Department Of Pharmaceutics,
A.M. Reddy Memorial College of Pharmacy,
Narasaraopet, Guntur (district), Andhra Pradesh
7yedu7@gmail.com

ABSTRACT:
Novel Oral Drug Delivery technologies have emerged and expanded into different drug delivery System. Among them Pulsatile Drug Delivery Systems are gaining a lot of interest as they deliver the drug at the right place at the right time and in the right amount, thus providing spatial and temporal delivery and increasing patient compliance. The drug delivery is designed according to the circadian rhythm of body. System is suit for the drugs which shows high first pass effect. A pulse has to be designed in such a way that a complete and rapid drug release is achieved after the lag time. Various systems like  Time controlled pulsatile release systems, Stimuli-induced pulsatile release system, Externally regulated pulsatile release system, Multiparticulate regulated pulsatile release system have been dealt with in the article. These delivery occurs at predetermined lag time. These systems are beneficial for the drugs having chronopharmacological behavior where night time dosing is required, such as anti-arhythmic and anti-asthmatic.

DRUG EXCIPIENT COMPATIBILITY STUDIES USING THERMAL METHODS

ABOUT AUTHORS:
Sandeep Kumar Patro*, M.Jhansi, Dr.A.M.S.Sudhakar Babu, Sk.Asief, P.Ramesh Babu
A.M.Reddy memorial college of pharmacy, Narasaraopet.
Sandeepkumarpatro2@gmail.com

 

ABSTRACT
Study of drug - excipient compatibility is an important process in development stage of dosage forms. Incompatibility between drugs and excipients alter drug stability and bioavailability &there by affect their safety and efficacy. Dosage form is a pharmaceutical drug delivary system, which is a combination of drug(s) and non-drug  components called as excipients. Drug is a chemical substance obtained from either natural, synthetic or semi synthetic source, which is used for the treatment, curing, prevention  or mitigation of a disease or disorder in human beings or animals Excipients are nondrug components which are serve specific purposes like shape, stability, solubility, elegance, palatability etc. of a dosage form. These are also called as adjuvants, additives or pharmaceutical aids. The evaluation of drug-excipient compatibility is therefore an essential aspect of any preformulation study. To evaluate the drug- excipient compatibility different techniques such as  Thermal analysis, Differential scanning  calorimetric (DSC) study, Infra red spectrophotometric study (IR), Isothermal stress testing (IST), High performance liquid chromatography (HPLC),Thin layer chromatography (TLC), Solution calorimetry  were adopted.

LIPOSOMES AN OVERVIEW– A NOVEL TREND IN THE DRUG DELIVERY

ABOUT AUTHORS:
*Sambhara Gayatri Deepti1, Aruna Ragidi1, A.M.S.Sudhakar Babu1, P.Venkateswara Rao2
1Department of Pharmaceutics
2Department of Pharmaceutical Analysis
A.M.Reddy Memorial college of Pharmacy, Narasaraopet, Guntur Dist., Andhra Pradesh, India.
gayatri.dpt11@gmail.com

ABSTRACT:
Liposomes are result of self assembly of phospholipid in an aqueous media resulting in closed bilayered structures. Liposomes are one of unique drug delivery system which can be use in controlling and targeting drug delivery system. Liposomes are generally classified based upon structure, method of preparation, composition and application, conventional liposome, and specialty liposome. Liposomes are formulated and processed to differ in size, composition, charge and lamellarity, depending upon method of preparation either active loading technique or passive loading technique. The prepared liposomes are characterized for visual appearance, liposomal size distribution, lamillarity, liposome stability, entrapped volume and surface charges. Different marketed formulations are available in market for liposomes. The liposomes have many applications which increase its importance over other formulations.

BIO-ANALYTICAL METHOD DEVELOPMENT, VALIDATION AND TRANSFER BY USING LC-MS/MS – A REVIEW

About Authors:
Satya Lakshmi.B*, P.Raju vel, Dr. P. Venkateswara Rao, Sindhu.G, Nikil Kumar.K
A.M Reddy Memorial College of Pharmacy,
Narasaraopet, AN University, Guntur.
balla.satya03@gmail.com

Abstract:
The reliability of quantitative assays in determination of drugs in biological fluids using High-performance liquid chromatography with Tandem Mass spectrometric determination (LC-MS/MS) detection methods and the integrity of resulting Pharmacokinetic data may not be absolute, in contrary to common perceptions and possible conjectures. The results may be adversely affected by lack of specificity and selectivity due to ion suppression caused by the sample matrix and interferences from metabolites. The advancements in the past few years and new technologies introduced can be used in enhancing LC-MS/MS Bio-analytical method development by reducing matrix effects. This Article reviews Automated Sample preparation and various extraction techniques like liquid-liquid extraction, Solid phase extraction and protein precipitation which plays an important role in sample preparation and detection by LC-MS/MS. Potential drawbacks during method development and validation are pointed out.

METHODS FOR THE ESTIMATION AND VALIDATION OF MULTICOMPONENT FORMULATION

About Authors:
B. Madhavi*, Dr. P.Venkateswara Rao, P.Rama Bharathi, T.Swathi, BH. Ramya Reddy
A.M Reddy Memorial College of Pharmacy, Narasaraopet
ANU University, Guntur.
*madhavi.pharm@gmail.com

Abstract:
The aim of the present work was focused on development of analytical methods for the estimation of drugs in multi component dosage form. There is a plethora of analysis of such formulations without prior separation. For the estimation of multi component formulation, the instrumental techniques, which are commonly employed, are spectrophotometry, Gas liquid chromatography (GLC), high performance thin layer chromatography (HPTLC), high performance liquid chromatography (HPLC) etc. These methods are based upon the measurement of specific and non specific physical properties of the substances. Chromatographic separation techniques are one of the most widely used technique for analysis of a multi component formulation. HPLC techniques allows for the separation as well as analysis of different drugs that are present in a combined formulation. Validation studies were performed in order to assess the validation parameters for the analytical method developed in accordance to ICH Guidelines.

COLON TARGETED DRUG DELIVERY SYSTEMS: A REVIEW

ABOUT AUTHORS:
P.Ramaa Bharathi*, Dr A.M.S.Shdhakar Babu
Department of Pharmaceutics,
A.M.Reddy Memorial College of pharmacy, Narasaraopet,
Guntur (Dt), Andhra Pradesh, India.
*puluguramabharathi63@gmail.com

ABSTRACT
The colon is a site where both local and systemic delivery of drugs can take place. Local delivery allows topical treatment of inflammatory bowel disease. However, treatment can be made effective if the drugs can be targeted directly into the colon, thereby reducing the systemic side effects. The colon targeted drug delivery has a number of important implications in the field of pharmacotherapy. Oral colon targeted drug delivery systems have recently gained importance for delivering a variety of therapeutic agents for both local and systemic administration. Targeting of drugs to the colon via oral administration protect the drug from degradation or release in the stomach and small intestine. It also ensures abrupt or controlled release of the drug in the proximal colon. Various drug delivery systems have been designed that deliver the drug quantitatively to the colon and then trigger the release of drug. This review will cover introduction, colon targeted diseases, drugs and sites, criteria for selection of drugs to CDDS, factors to be considered in the design, advantages, disadvantages, colonic absorption, factors affecting colonic absorption, approaches used for site specific drug delivery to colon and different types of polymers which can be used in formulation of colon targeted drug delivery systems.

DRIED BLOOD SPOT SAMPLING TECHNIQUE IN QUANTITATIVE ANALYSIS OF SMALL MOLECULES- A REVIEW

ABOUT AUTHORS:
Bhimavarapu Ramya Reddy*1, Bhavna Priyasri S2
*1Department of Pharmaceutical Analysis, A.M Reddy Memorial College of Pharmacy, Narasaraopet, Guntur, Andhra Pradesh, India
2Department of Pharmaceutical Engineering, New Jersey Institute of Technology, New Jersey, U.S.A.
ramyareddy.bh@gmail.com

ABSTRACT:
Dried blood spots (DBS) as an attractive alternative to conventional venous plasma sampling in many pharmaceutical companies and clinical laboratories, different analytical approaches have been developed to enable automated handling of DBS samples without any pretreatment. DBS offers a number of advantages over conventional blood collection. As a less invasive sampling method, DBS offers simpler sample collection and storage and easier transfer, with reduced infection risk of various pathogens, and requires a smaller blood volume. DBS-LC-MS/MS has emerged as an important method for quantitative analysis of small molecules. This technique is Widely used to screen for metabolic problems in newborn babies, PK studies. The objective of this review is to describe the analytical concepts of current direct DBS techniques along with DBS sample collection, processing and storage and to present their advantages and disadvantages.

DETERMINATION OF PURITY AND RELATIVE MOLECULAR WEIGHT OF PURIFIED HUMAN IgG

About Authors:
1M Prasad Naidu, 2Dr Madhu Sudan Reddy, 3T Madhu Chaithanya, 4N Mallikarjun Rao

1(Medical Biochemistry) NMCH, Nellore, AP, India.
2(MD Pharmacolgy) NMCH, Nellore, AP, India.
3(Medical Pharmacology) MIMS, Vijayanagaram, AP, India.
4(MSc Botany). Acharya Nagarjuna University, Guntur, AP, India.
*www.prasadnaidu.com@gmail.com

Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)
An important technique for the separation of proteins is based on the migration of charged proteins in an electric field, a process called electrophoresis. These procedures are not generally used to purify proteins in large amounts, because simpler alternatives are usually available and electrophoretic methods often adversely affect the structure and thus the function of proteins. Electrophoresis is, however, especially useful as an analytical method. Its advantage is that proteins can be visualized as well as separated, permitting a researcher to estimate quickly the number of different proteins in a mixture or the degree of purity of a particular protein preparation. Also, electrophoresis allows determination of crucial properties of a protein such as its isoelectric point and approximate molecular weight. The polyacrylamide gel acts as a molecular sieve, slowing the migration of proteins approximately in proportion to their charge-to-mass ratio. Migration may also be affected by protein shape. In electrophoresis, the force moving the macromolecule is the electrical potential, E. The electrophoretic mobility of the molecule, µ, is the ratio of the velocity of the particle molecule, V, to the electrical potential. Electrophoretic mobility is also equal to the net charge of the molecule, Z, divided by the frictional coefficient, f, which reflects in part a protein’s shape.

CONSIDERATIONS FOR BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY USING LC-MS/MS-A REVIEW

ABOUT AUTHOR:
Bhimavarapu Ramya Reddy
Department of Pharmaceutical Analysis,
A.M Reddy Memorial College of Pharmacy, Narasaraopet,
Guntur, Andhra Pradesh, India.
ramyareddy.bh@gmail.com

ABSTRACT:
One of the major challenges facing the pharmaceutical industry today is to increase the productivity, cost effective, ultimately developing new therapies that increases the human health. In order to achieve these challenges, new hyphenated analytical techniques has been employed to perform experiments in less span of time with high quality. During last decade, quantification of low molecular weight drugs by using LC-MS/MS in biological fluids is common procedure in many clinical and preclinical laboratories. Also it plays significant role in the evaluation and interpretation of bioequivalence, pharmacokinetic, and toxicokinetic studies. This overview highlights a number of issues involving “small molecule drugs”, bioanalytical liquid Chromatography –tandem mass spectrometry, which are frequently encountered during assay development.

Pages