What is Chromatography?
- Chromatography is the technical separation of substance according to their Partition Coefficient in two immiscible phases.
- Basically, all chromatographic system consists of a stationary phase , which may be as solid or liquid that is immobilized and the mobile phase which may be liquid or gas, flows over or through stationary phase.
- The various components in a sample get separated essentially due to their differences in their partition or distribution behavior between the stationary phase and the mobile phase.
- This distribution is measured by Partition Coefficient which is defined as the ratio of concentration of compound in two phases at equilibrium.
Presentation by,
Deepali R. Khangaonkar, Dr. Vinod S. Narayane
Birla College (Mumbai university)
Mumbai, Maharashtra
Email: deepalikhangavkar@gmail.com
.png)
