Hyderabad

CANCER A DARKER SIDE OF LIFE

About Authors:
PIYUSH PARASAR
SPS I.T.E.R BHUBANESWAR,
INDIA

INTRODUCTION- 
CANCER A DARKER SIDE OFLIFE LITERALLY MEANS A HOPE TO LIVE FURTHER GET DIMINISHED IN THE CASE OF CANCER. MAN HAS BEEN EXPOSED TO THE CHANGING SURROUNDING EVER SINCE HE CAME INTO EXISTENCE.IN THE PROCESS HE IS VULNERABLE TO THE NUMBER OF FATAL DISEASE AMONG WHICH CANCER IS A DREADED MALADY NEXT TO CARDIOVASCULAR  DISEASE. IT IS KNOWN TO ALL OF US THAT DEATH IS INEVITABLE BECAUSE IT IS A VICIOUS CYCLE OF THE NATURE. BUT THIS TYPE OF ESOTERIC DISEASE MAKES OUR LIVES FROM SIMPLE TO COMPLEX. SOME PEOPLE BELIEVE IN THE CONCEPT OF AS YOU SOW SOW YOU SHALL REAP ESPECIALLY IN INDIA. THESE ARE ALL SPECULATIONS OF OUR WAY OF THINKING AND THOUGHTS. IN MAJORITY OF CASES WE HAVE SEEN THAT DUE TO OUR MODERN LIFESTYLE WE GET SUFFERED FROM THESE TYPE OF DISEASE.

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Isolation And Molecular Characterization Of Xylanase Enzyme From Soil

About Author:
E.Ashwini
Microbiologist  In  Institute Of Health Systems, Hyderabad
M.Sc Microbiology From Osmania University.

ABSTRACT
The purpose of this study was to determine the effect of some cultural conditions on the xylanase enzyme production by two Isolated Species from the industrial soiland to investigate its potential to produce xylanase utilizing tomato pomace as a substrate. Xylanase activity was detected using the Dinitrosalicylic acid assay method.
The Alkalophilic bacteria isolated from the industrial soil, secreats extra cellular xylanases when grown in liquid media supplemented with eighter rice bran, grass, corn cob, or sugar baggage as a carbon sources (which were treated with 2N NaoH for removing the cellulose from these substrates). The two bacteria belonging to the species Sporo lactobacilli and Acrobacter respectively shows the high enzyme activity at high temperatures 50?C and 60?C and high enzyme activity was found at pH 8 and pH 9 for two organisms. The extra cellular enzyme has an apparent molecular weight of 66 KD & 67KD for both the organism respectively, as determined by SDS-PAGE. The purified enzyme has two peptides and was conformed by Zymogram analysis. The species sporo lactobacilli show high enzyme activity of 4.7 U/ml and the species Acrobacter shows the enzyme activity of 7.46 U/ml.

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