QUANTITATIVE DETERMINATION OF ASENAPINE MALEATE USING REVERSE PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
About Authors:
T.R.PARTHASARATHI*, M.VANITHA SRI.
BIOANALYTICAL DEPARTMENT,
QUEST LIFE SCIENCES PVT. LTD, SDF III, MEPZ,
TAMBARAM, CHENNAI- 600 045, INDIA.
*parthu_14@yahoo.co.in
ABSTRACT :
A novel isocratic reverse-phase high performance liquid-chromatography method for determination of asenapine maleate was developed and validated after optimization of various chromatographic conditions. Samples were separated on a waters x-terra C18 (100 mm × 4.6 mm, 3.5 μ) analytical column. The mobile phase used was acetonitrile: 0.1M phosphate buffer (pH 3.2) 65:35%v/v operated at 30 °C column oven temperature was pumped at a flow rate of 1.0 mL min−1 and the column eluents were monitored at a wavelength of 272 nm. When sample was injected into the Finnigan surveyor high performance liquid-chromatography system through Finnigan surveyor auto-sampler injector, separation was achieved within 5.0 min. The present method demonstrated was validated with the acceptable values for selectivity, linearity (within the expected concentration range (10–50 μg mL−1; r2 > 0.999)), recovery (>95%), precision (%RSD < 2.0), sensitivity (limit of detection: 1.85 µg mL−1 and lower limit of quantification: 2.34 µg mL−1), robustness, and ruggedness.
Southern Railway, in its present form, came into existence on 14th April 1951 through the merger of the three state railways namely Madras and Southern Mahratta Railway, the South Indian Railway, and the Mysore state railway.
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