Development and Validation of HPTLC Method for Estimation of Tapentadol Hydrochloride

  • Posted on: 1 October 2014
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PharmaTutor (October- 2014)

 

ISSN: 2347 - 7881
(Volume 2, Issue 10)

 

Received On: 05/08/2014; Accepted On: 07/08/2014; Published On: 01/10/2014

 

AUTHORS: Shoumik Roy*, Samil D. Desai, Bhavna A. Patel, Shraddha J. Parmar
Post Graduation Department of Pharmaceutical Sciences,
Sardar Patel University, Vallabh Vidyanagar, Gujarat, India.
roy.shoumik@gmail.com

 

ABSTRACT:
The main thrust of the paper was to develop and validate a simple, precise high performance thin-layer chromatographic (HPTLC) method for estimation of tapentadol hydrochloride in tablet dosage form. Chromatography was performed on silica gel 60 F254 plates with Chloroform: Acetone: Ammonia (2.5: 2.4: 0.1 v/v/v) as mobile phase. This mobile phase system gave a good resolution for tapentadol hydrochloride (Rf value of 0.49 ± 0.02). Detection and quantification were carried out at 272 nm. The linear regression data for the calibration plot showed a good relationship with r=0.999. The limits of detection and quantification were 62.68 and 189.94ng/spot for tapentadol hydrochloride. The amounts of the drugs in the marketed formulation were 99.98%.

 

How to cite this article: S Roy, SD Desai, BA Patel, SJ Parmar; Development and Validation of HPTLC Method for Estimation of Tapentadol Hydrochloride; PharmaTutor; 2014; 2(10); 136-141

 

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