Development and Validation of HPTLC Method for Estimation of Tapentadol Hydrochloride
PharmaTutor (October- 2014)
ISSN: 2347 - 7881
(Volume 2, Issue 10)
Received On: 05/08/2014; Accepted On: 07/08/2014; Published On: 01/10/2014
AUTHORS: Shoumik Roy*, Samil D. Desai, Bhavna A. Patel, Shraddha J. Parmar
Post Graduation Department of Pharmaceutical Sciences,
Sardar Patel University, Vallabh Vidyanagar, Gujarat, India.
The main thrust of the paper was to develop and validate a simple, precise high performance thin-layer chromatographic (HPTLC) method for estimation of tapentadol hydrochloride in tablet dosage form. Chromatography was performed on silica gel 60 F254 plates with Chloroform: Acetone: Ammonia (2.5: 2.4: 0.1 v/v/v) as mobile phase. This mobile phase system gave a good resolution for tapentadol hydrochloride (Rf value of 0.49 ± 0.02). Detection and quantification were carried out at 272 nm. The linear regression data for the calibration plot showed a good relationship with r=0.999. The limits of detection and quantification were 62.68 and 189.94ng/spot for tapentadol hydrochloride. The amounts of the drugs in the marketed formulation were 99.98%.
How to cite this article: S Roy, SD Desai, BA Patel, SJ Parmar; Development and Validation of HPTLC Method for Estimation of Tapentadol Hydrochloride; PharmaTutor; 2014; 2(10); 136-141