TOTAL PHENOLIC AND FLAVONOID CONTENT OF DIFFERENT EXTRACTS OF INDIGOFERA TINCTORIA LINN

 

Preliminary phytochemical analysis of different extracts

S.No

CHEMICAL TEST

Aque
ous

Ethanol

Chloro
form

Ace
tone

Ethyl acetate

Pet ether

Hydro Alcoholic

1

Alkoloids

+

+

+

+

+

+

+

2

Glycosides

+

+

--

+

-

-

+

3

Flavonoids

+

+

+

+

-

-

+

4

Saponins

+

+

+

+

+

+

+

5

Steroids

+

-

-

-

+

-

-

6

Carbohy
drates

+

+

+

+

+

+

+

7

Protiens

+

+

-

-

-

-

+

8

Tannins

+

-

+

+

+

+

+

9

Phenolic Group

+

+

+

+

-

-

+

+ = present, - =Absent

Preliminary phytochemical analysis revealed that the presence of flavonoids and Phenolics abundantly. Besides this hydroalcoholic extracts shows presence of  alkaloids, glycosoide, proteins, saponons, carbohydrates, Tannins, Proteins.

In the present study, total phenolic content present in Indigofera tinctoria Linn extract was estimated using modified Folin- ciocalteau method and total flavonoid content by aluminium chloride assay method. The results of total phenolic and total flavonoid content  and ratio of total flavonoids/phenolic content of Indigofera tinctoria Linn were represented in table II. The data clearly indicating that Indigofera tinctoria Linn, medicinal herb is rich of phenols and flavonoids. Aqueous extract contains more phenolic and flavonoid content in comparision with other solvent extracts.

Conclusion:
Phenolics are the widely spread as secondary metabolites in plant kingdom(Aleemuddin MA et al, 2012). These are potential natural antioxidant and act by efficient radical scavengers and metal chelator(Tapas et al., 2008). Aqueous extract of Indigofera tinctoria Linnleaves and ethanolic extract of stem and root of Indigofera tinctoria Linnis a potential source of phenols and flavonoids. Further deeper studies have to be carried out to isolate new natural molecules.

Tables and figures:

Table 1 percentage of yield of Indigofera tinctoria Linnextract

Solvent

Yield of extract(%)

Aqueous

23.3

Ethanol

14.2

Ethyl acetate

3.3

Chloroform

3.8

Acetone

5

Petroleum ether

0.6

Table II Total phenolic and flavonoid content of leaves extract of Indigofera tinctoria

solvent

Total phenolics mg GAE/100g fresh mass

Total flavonoids mg QC/100g fresh mass

Flavonoids/Phenolics

Aqueous

73.48

55.9

1.31449

Ethanol

67.43

50.11

1.34564

Chloroform

8.09

2.2

3.677273

Acetone

5.93

3.85

1.54026

Ethyl acetate

25.29

18.99

1.331754

Petroleum ether

55.26

43.01

1.284817

Figure I standard calibration curve of Gallic acid

FigureII standard calibration curve of Quercetin

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