FORMULATION, EVALUATION OF POLYHERBAL SOLID DOSAGE FORM AND STUDY OF ITS ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY

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Table 4: Antifungal activity of polyherbal formulation

In characterization study of poly herbal capsule results showing that weight variation test (n=20) was with in IP limit, moisture analysis (n = 20) was 3.98+_0.5 and pH (n = 20) was 3.5 +_0.02 and drug content uniformity (n = 20) was within IP limit.
In vitro release of the developed formulation was studied and presented in Figure 1. The release rate of the polyherbal formulation showed a maximum cumulative release of 91 % at the end of the study (120 min.)

In dissolution study the result showing almost 100% drug release at 2 hrs. It may due to poly herbal drugs complexity and need for further studies to improve the drug release.

DPPH radical scavenging or the antioxidant activities of poly herbal extract and Quercetin (taken as the standard) were presented in table I. The poly herbal extract has expressed a noteworthy dose dependent inhibition of DPPH. This influence of antioxidants on DPPH is due to its ability to donate hydrogen. The half inhibition concentration (IC50) of poly herbal extract and Quercetin were 27.07μg/ml and 6.248μg/ml respectively. The potential of Quercetin and poly herbs to scavenge DPPH radical is directly proportional to the concentration. The DPPH assay activity is near to standard as Quercetin.

In this polyherbal capsule containing nine herbs are edible vegetables and each one having natural antioxidant effect and they are defend against oxidative imbalance. The known fact behind in most of the end organ damage is reactive oxygen species followed by oxidative stress. This can be balanced by this poly herbal capsule.

Antibacterial activity of poly herbal extract, Ciprofloxacin (Standard) and Fluconazole (standard for antifungal activity) are presented in table 2. The poly herbal extract has showing good response against E. coli (antibacterial) and Aspergillus niger (antifungal) with zone of inhibition of 34 mm.and12mm. Respectively when compared to the standard drugs (33 mm and 12 mm respectively).  Rest of other microbesthe zone will be moderately inhibited by poly herbal extract when compared to the standard drugs.

Out of nine herbs particularly Neem, Moringa and Ginger possess antibacterial and anti-fungal activity. The rest of the herbs may be potentiate the anti-microbial activity of Neem and others which is reflected in this study by the zone of inhibition almost equal to standard drug particularly E.Coli and Aspergillus niger.

CONCLUSION
Polyherbal capsule are compact formulation and follow the long way treatment strategies to treat the many disorder like cardiovascular, cancer, inflammatory, ulcer, diabetes and infectious. Also polyherbs are improving the immune system and iron level in hemoglobin. In this study result showed that antioxidant and antimicrobial efficacy and further study need to be focused on preclinical evaluation for pharmacological finding.

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