Antimicrobial activity of Cymbopogon Species against Human Pathogens

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Rudra Sharan Dwivedi *
Bhopal University,
Working in RMS Regrow, Pune

, is a tall perennial grass with 55 species ofgrasses. Lemongrass is the source of Lemongrass oil, natural citral, which is used as a basic raw material for synthesis of β-ionone used for synthesis of a number of useful aromatic compounds and Vitamin-A. In the present study the determined plant parts (5g) of four CymbopogonSps. were ground, extracted with five solvents and filtered after 48hrs. The dry weight of the extracts was obtained by allowing the solvent to evaporate and was used to determine concentration in mg/ml. Microbial susceptibility assays using the agar dilution (Mueller-Hinton Agar) method (%v/v and corresponding mg/mL values) and the Minimal Inhibitory Concentration (MIC) were carried out for E.coli, Bacillus sp., Streptococcus sp., Staphylococcus sp.


The plants that possess therapeutic properties or exert beneficial pharmacological effects on the animal body are generally designated as “Medicinal Plants” ( Medicinal plants are gifts to nature to cure limitless number of diseases among human beings. The abundance of plants on the earth’s surfaces has led to an increasing interest in the investigation of different extracts obtained from traditional plant as potential sources of new antimicrobial agents (Bonjor et al., 2004). Natural antimicrobials can be derived from plants, animal tissues, or microorganisms (Gordon et al., 2001).  Medicinal plants and their parts contains different chemical substances that can be used for therapeutic purpose or which are precursors for synthesis of useful drugs. 

Medicinal plants synthesize substances that are useful to the maintenance of health in humans and other animals. These include aromatic substances, most of which are phenols or their oxygen-substituted derivatives such as tannins. Many are secondary metabolites, of which at least 12,000 have been isolated a number estimated to be less than 10% of the total ( Secondary metabolites are organic compounds that are not directly involved in the normal growth, development or reproduction of organisms. Plants synthesize a bewildering variety of phytochemicals such as alkaloids, phenolics, terpenoids, glycosides etc.Lemon grass oil contains geraniol, myrcene, citronellal, limonene, linalool and dipentene and none of the constituents were reported to be mutagenic with Salmonella assay. (Gomes et al., 1998)

Cymbopogon, a tall perennial grass has 55 species. Cymbopogon is the source of Lemongrass oil, a good source of natural citral, which is used as a basic raw material for synthesis of β-ionone used for synthesis of a number of useful aromatic compounds and Vitamin-A. Lemon grass is a long thick grass with leaves at the top and a solid portion several inches long at the root end. Lemon grass used for culinary purposes, drugs, flavoring, insect repellants, liquors and perfumes. The lemon grass oil is used in the manufacture of perfumes of soaps, hair oils, scents and medicines. It also have antibacterial properties. Ionone prepared from the Citral present in lemon grass oil is one of the most important raw material for the preparation of vitamin-A( antimicrobial is a substance that kills or inhibits the growth of microbes such as bacteria, fungi, or viruses. 

There are several methods used to extract plant secondary metabolites (example: antimicrobial compound) which includes distillation, steam distillation, hydro distillation, water and  steam distillation, solvent extraction, carbon dioxide extraction, cold pressing, florasols/phytols, maceration. Solvent extraction involves separating the constituents of a mixture using a volatile solvent ( ethanolor hexane ) that does not mix with water. The solvent takes up the molecules to be extracted because it has a strong affinity with them. To collect the active ingredients, the solvent is then removed by distillation ( Panna et al;2008)

In this article, I give an account of the antimicrobial activity of 4 differerent species of Cymbopogon (Cymbopogon citratus, Cymbopogon flexuosus, Cymbopogon martini and Cymbopogon winterianus)

Sample collection:  Medicinal plants such as Cymbopogon citratus, Cymbopogon flexuosus, Cymbopogon martini and Cymbopogon winterianus were collected from different nursery of Bhopal.

Solvent extraction: 5gm of plant leaves were taken and dried under shade. Dry weights of the leaves were weighed and crushed using mortar and pestle. The crushed plant leaves were extracted with 25ml of different solvents like ethanol, methanol, propanol, acetone and diethyl ether. The crushed plant material kept in rotator shaker along with solvents for 2 days. After incubation, solvents were filtered using Whatmann No.1 filter paper. The residue after filtration was dried and weighed.

Collection of microorganisms:   The strains used in this work were isolated from patients suffered with bacterial diseases. The isolates were identified by Gram staining, biochemical characterization and by using selective media. The organisms isolated were E.coli, Bacillus sp., Staphylococcus sp. and Streptococcus sp. The microorganisms were grown overnight at 370C   in selective media such as, E.coli  - EMB agar, Bacillus sp. – Nutrient agar,  Staphylococcus sp.– Mannitol salt agar,Streptococcus sp. – Blood agar.

For identification and screening Gram’s staining was done. The morphologically identified organisms were grown on selective media such as, EMB agar, Starch agar, Mannitol salt agar and Blood agar .These selectively grown isolates were used for biochemical characterization by indole production, methyl red, Voges-Proskauer, citrate, SIM agar, gelatin, catalase, oxidase, Urease, nitrate tests.

Determination of antimicrobial activity:  Antimicrobial activity was determined by agar diffusion method. Three to five identical colonies of E.coli, Bacillus sp., Staphylococcus sp., and Streptococcus sp., from each agar plate were lifted with a sterile loop and transferred into a tube containing 5ml of nutrient broth. All the bacteria mentioned above were incubated at 37° C for 24hours. The Mueller Hinton agar was sterilized and cooled to 45-50° C and then the fresh cultures of the microorganisms (0.1ml) were inoculated. Each inoculated petridishes were swirled to distribute the medium homogeneously and allowed to dry for 15-20minutes. Wells (6) of 7mm were made into previously seeded MH agar plates. Each well was filled with 50µl of each plant extract. The same quantity of 75% ethanol served as control. The plates were allowed to stand at room temperature for 1hour for extract to diffuse into the agar and then they were incubated at 37° C for 24hours. Subsequently, the plates were examined for bacterial growth inhibition. The diameter of cleared zones was measured in cm. the resulting zone diameter is shown in the table. The transparently cleared zones containing micro colonies showed bacteriostatic activity.

Determination of minimal inhibitory concentration
This test was performed on the bacteria that exhibited inhibition zones using the effective plant extracts. The extracts that exhibited considerable activity when used in different concentration like 10µl, 20µl, 30µl, 40µl. The ethanol, methanol, propanol, acetone and diethyl ether extracts are used for the assay of MIC against E.coli, Bacillus sp., Staphylococcus sp., and Streptococcus sp. The test culture was inoculated with MH agar and poured in petriplates. The different concentration of each extract was poured in the wells. Then the plates were incubated at 37°C for 24hours. The lowest concentration that did not permit any visible growth, it was considered as the minimum inhibitory concentration.  

Medicinal Use
 is used extensively in Ayurvedic Medicine. It is normally used for common cold with headache, diarrhea, wind-cold impediment pain, cold pain in stomach duct and abdomen, knocks and falls.


  1. Anjaria, J., Parabia, M., Dwivedi, S. (2002). Ethnovet  Heritage Indian Ethnoveterinary Medicine – An Overview, Pathik Enterprise, Ahmedabad , India .
  2. Balandrin, M.F.J., Kjocke, A., Wurtele, E.(1985). Natural plant chemicals sources of industrial and medicinal materials. 228:1154-1160.
  3. Bonjar, Glls and Farrokhi, P.R. (2004). Antibacillus activity of some plants used in traditional medicine of Iran.Niger. J. Nat. Prod. Med., 8: 34-39.
  4. Bushra Beegum, N.R., Ganga Devi, T (2003). Antibacterial activity of selected Seaweeds from Kovalam southWest coast of India . Asian Jr. of Microbiol. Biotech. Env. Sc. 5(3): 319-322.
  5. Carbajal, D., Casaco, A., Arruzazabala, L., Gonzalez and Tolon,Z. (1989). Pharmacological study of Cymbopogon citratesleaves. J. Ethnopharmacol. 25(1): 103-107.
  6. Carlini, E.A., Contar, J.D.D.P., Siva-Filho, A.R., Dasilveira-Filho, N.G., and Frochtengarten (1986). Pharmacology of lemongrass (Cymbopogon citratus ) Effects of teas prepared from the leaves on laboratory animals. J.Ethnopharmacol. 17: 37-64.
  7. Dagmar Janovska., Katerina Kubikova., Ladislav kokoska.(2003).Screening for antimicrobial activity of some medicinal plant species of traditional Chinesemedicine. Czech.J.Food.Sci. 21(3):107-110
  8. Duraipandiyan, V., Ayyanar, M., Ignacimuthu, S.(2006). AntimicrobialActivity of Some Ethnomedical Plants Used by Paliyar Tribe from TamilNadu , India . BMC complementary and alternative medicine.  635.
  9. Eloff, J.N. (1988) Which extract and should be used for the screening and isolation of antimicrobial components from plants.Journal of Ethnopharmacology.60:1-8.
  10. Erdo Urul, O.T. (2002).Antibacterial activities of some plant extracts used in folk medicine. Pharmaceutical Biol .40: 269-273
  11. Esimone, C. O., Adiukwu, M. U. and Okonta, J. M. (1998). Preliminary Antimicrobial Screening ofthe Ethanolic Extract from the Lichen Usnea subfloridans (L). Journal of Pharmaceutical Research and Development. 3(2): 99 – 102.
  12. Essawi, T., Srour, M. (2000) .Screening of some Palestinian medicinal plants for antibacterial activity.Journal of Ethnopharmacology .70: 343-349.
  13. Ferrua, F.Q., Marques, M.O.M., Meireles, M.A.A.,Óleo Essencial de Capim-Limao obtido por Extraçao com Dióxido de Carbono Líquido.Ciência e Tecnologia de Alimentos. 14: 83(1994).
  14. Gulluce, M., Sökmen, M., Daferera, D., Agar, G., Özkan, H., Kartal, N., Polissiou, M., Sökmen, A. and Sahin, F. (2003). In-vitro antibacterial, antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agriculture and Food Chemistry 51(14): 3958 -3965.
  15. Hammer, K.A., Carson , C.F., Riley, T.V. (1999) Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology.86: 985-990.
  16. Hatch, E. (1995). Asthma, inhaled oxidants, and dietary antioxidants. Am.   J. Clin. Nutr. 61(3): 625-630.
  17. Iwu, M.W., Duncan , A.R., Okunji, C.O. (1999).New Antimicrobials of Plant Origin. In:     Janick, J. (ed.): Perspectives on New Crops and New Uses. ASHS Press, Alexandria , VA : 457–462.
  18. Iyengar, M.A.( 1995). Study of Crude Drugs. Manipal Power Press, Manipal , India . pp 2.
  19. Jigna Parekh, Darshana Jadeja, Sumitra Chanda.(2005). Efficacy of Aqueous and Methanol Extracts of Some Medicinal Plants for Potential Antibacterial Activity.Turk.J.Biol 29:203-210.
  20. Lorenzetti, B.B.; Souza, G.E.P.; Sarti, S.J.; Filho, D.S.; and Ferreira, S.H. Myrcene mimics the peripheral analgesic activity of lemongrass tea. J. Ethnopharmacol. 1991, 34, 43-48.
  21. Mishra, A.K. Dubey, N.K. (1994). Evaluation of some essential oils for their toxicity  against fungi causing deterioration of stored food commodities. Appl. Environ. Microbiol. 60: 1101-1105.
  22. Nair, R., Kalariya, T., Sumitra Chanda.(2005). Antibacterial Activity of Some Selected Indian Medicinal Flora, Department of Biosciences, Turk J Biol. 29: 41-47.
  23. Ogueke, C.C., Ogbulie, J.N., Okoli, I.C. and Anyanwu, B.N. (2007).Antibacterial Activities and toxicological potentials of crude ethanolic extracts of Euphorbia hirta.Journal of American Science. 3(3): 11-16.
  24. Osadebe, P. O., Ukwueze, S. E. (2004). A Comparative Study of the Phytochemical and Antimicrobial Properties of the Eastern Nigerian Species of African Mistletoe (Loranthus micranthus) sourced from different host trees. Journal of Biological Research and Biotechnology. 2(1): 18 – 23.
  25. Ozer, N.K., Boscoboinik, D. and Azzi, A. (1995). New roles of low density lipoproteins  and vitamin E in the pathogenesis of atherosclerosis.Biochem Mo! Biol Int. 35: 117-24.
  26. Panna Thapa.(2008). Phytochemical and  antimicrobial evaluations of some medicinal plants of Nepal . Department of science.1(5):49-54.
  27. Parasakthy, K., Shanthi, S., Deepalokshmi, P., Niranjali , S.D. (1996). The antioxidant effect of eugenol and carbon tetrachloride induced erythrocyte damage in rats. J.Nutr. Biochem. 7: 23-28.
  28. Ress NB (2003). Toxicology and carcinogenesis studies of microencapsulated citral in rats and mice. Toxicol. Sci.71(2): 198-206.
  29. Surh, Y. (1999). Molecular mechanisms and chemopreventive effects of selected dietary and medicinal phenolic substances.Mut. Res. 428: 305-327.
  30. Tomoko, N., Takashi, A., Hiromu, T., Yuka,I., Hiroko, M., Munikazu, I., Totshiyuki, T., Tetsuro, I., Fujio,A.,Iriya, I. , Tsutomu, N., Kazuhito, W.(2002). Antibacterialactivity of extracts preparated from tropical andsubtropical plants on methicillin-resistantStaphylococcus aureus. J. Health Sci.,48: 273–276.
  31. Unningham, A. B.( 1988). An investigation of the herbal medicine trade. Natal/Kwa Zulu. Investigation Report.pp 79
  32. Van Den Berghe, D.A., Vlietinck, A.J. (1991). Screeningmethods for antibacterial and antiviral agentsfrom higher plants.Methods in Plant Biochemistry. 6: 47–69.
  33. Wagnor H., Hikino, H. and Farnsworth, N. R. (1989).Principle PP. The economic significance of plants and their constituents as drugs. In: Economic and medicinal plant research. 3:1-17.
  34. Zakaria, M. (1991) Isolation and characterization of active compounds from medicinal plants. Asia Pacific Journal of Pharmacology. 6: 15-20.




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