ANTI-ANEMIC ACTIVITY OF HYDRO-ALCOHOLIC EXTRACT SEEDS OF FOENICULUM VULGARE IN PHENYLHYDRAZINE INDUCED ANEMIC RATS
CHANDRAKANTA KUSHWAH 1*, DEEPANSHU GUPTA 1, ANKUR JOSHI 1, SAPNA MALVIYA 1 & ANIL KHARIA 1
1 Department of pharmacy Modern institute of Pharmaceutical Sciences,
Indore (M.P.), India
The main objective of this research was to evaluate the anti-anemic activity in hydro-alcoholic extract of seeds of Foeniculum vulgare in phenylhydrazine induced anemic rats. Phenylhydrazine (60mg/kg) was given intraperitoneally in rats for two days to induce anemia. The animal were divided into 5 groups of 6 animal each. Group 1 was known as normal control group, Group 2 was known as anemic control group, Group 3 was known as standard reference control group given with Vit. B12, Group 4 was known as test control-I given with 100mg/kg of hydro-alcoholic extract of seeds of Foeniculum vulgare, Group 5 was known as test control-II given with 200mg/kg of hydro-alcoholic extract of seeds of Foeniculum vulgare. All the test drugs were given for 28 days through oral route once in a day. On 29th day blood was taken out through tail puncture and was subjected to the determination of RBC, Hb and percentage Haematocrit. Both the hydro-alcoholic seeds extract of Foeniculum vulgare and Vit. B12 significantly increase the HB, RBC & percentage Haematocrit level which shows that Foeniculum vulgare seeds exhibits the anti-anemic activity
Reference Id: PHARMATUTOR-ART-2596
Anemia affects the lives of more than 2 billion people globally, accounting for over 30% of the world’s population which is the most common public health problem particularly in developing countries occurring at all stages of the life Cycle. Anemia is a condition that develops when blood lacks enough healthy red blood cells or haemoglobin. Iron deficiency is the most common nutritional disorder in which there is a depleted and a restricted supply of iron to various tissues and which becomes apparent. This may result in depletion of Hemoglobin and iron-dependent intra- cellular enzymes participating in many metabolic pathways. Plant and plant products are being utilized as a source of medicine since long. Plant extracts are used as phototherapeutics and are still a large source of natural antioxidants. Particularly, flavonoids and phenolics are considered as potential therapeutic agents. In many developing countries, herbal medicines are assumed as greater importance in health care (Mohan, 2005; http://www.who.int/topics/anaemia/en/ cited: 25-08-2017; Tortora, 2015).
Table 1- Plant profile
Fig. I - Seeds of Foeniculum vulgare
Fig. II - Plant of Foeniculum vulgare
Preparation of extract
The seeds were collected, shade dried and then converted into coarse powder. The powder was then filled in a Soxhlet apparatus for extraction by 70:30 hydro-alcoholic as a solvent. The Hydro-alcoholic extract was concentrated by vacuum distillation to dry. The collected extract was stored in suitable container and used for further pharmacological studies (Khandelwal 2013).
Wistar strain male albino rats, weighing 100–150 g were selected for the study. The animals were housed individually in polypropylene cages under hygienic and standard environmental conditions (22 ± 3°C, humidity 30–70%, 12 h light/dark cycle). The animals were allowed to have stan¬dard feed and water adlibtum. They were acclimated to the environment for one week prior to experimental use. All the animal testing were done under the approval of Institutional Animal Ethical Committee (IAEC) of Modern Institute of Pharmaceutical Sciences, Indore the approval no. is IAEC/MIPS/01/2017/02 (Hussain et al, 2017).
Anemia was induced by intra peritoneal injection of phenyl hydrazine at 60 mg/kg for 2 days,
Following the injections, rats were divided into five groups of six rats each.
Group I-Control rats received 0.1% Carboxy methyl cellulose.
Group II-Phenyl hydrazine treated rats (60 mg/kg per day for 2 days).
Group III-Phenyl hydrazine treated rats with Vitamin B12 per day for 28 days.
Group IV-Phenyl hydrazine treated rats with a single dose of seed extract of Foeniculum vulgare (100 mg/kg) per day for 28 days.
Group V-Phenyl hydrazine treated rats with a single dose of seed extract of Foeniculum vulgare (200 mg/kg) per day for 28 days.
On completion of the experimental period, the blood was collected with EDTA as an anticoagulant. Plasma was separated by centrifugation. Then Plasma was used for the estimation of various biochemical parame¬ters like Haemoglobin, RBC and percentage Haematocrit.
Data’s were expressed as mean ± SEM. The data were analysed by using one way analysis of variance (ANOVA) followed by Dunnet’s ‘t’ test. P values < 0.05 were considered as significant (Joshi Ankur et al, 2017; Joshi Ankur et al, 2017, Gupta Deepanshu et al, 2018).
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