You are hereAphrodisiac Activity of Bombax Ceiba Linn. Extract in Male Mice

Aphrodisiac Activity of Bombax Ceiba Linn. Extract in Male Mice


About Author: 1) Pankaj H. Chaudhary, M.Pharm
Dept. of Pharmacognosy, Government College of Pharmacy,
Kathora Naka, Amravati – 444604, Maharashtra, INDIA.
2) Somshekhar S. Khadabadi*. M.Pharm., PhD.
Dept. of Pharmacognosy, Government College of Pharmacy,
Kathora Naka, Amravati – 444604, Maharashtra, INDIA.

Abstract
In the present study, the aphrodisiac activity of Bombax ceiba Linn. (Bombacaceae) root extract was investigated. The extract (400 mg/kg body wt./day) was administered orally by gavage for 28 days. Mount latency (ML), intromission latency (IL), ejaculation latency (EL), mounting frequency (MF), intromission frequency (IF), ejaculation frequency (EF) and post-ejaculatory interval (PEI) were the parameters observed before and during the sexual behavior study at day 0, 7, 14, 21, and 28. The extract reduced significantly ML, IL, EL and PEI (p < 0.05). The extract also increased significantly MF, IF and EF (p < 0.05). These effects were observed in sexually active and inactive male mice.

Reference ID: PHARMATUTOR-ART-1120

Introduction
Aphrodisiacs are substances that stimulate/increase sexual desire and performance. There are numerous reports of aphrodisiac activity attributed to plants (Chandler, 1988; Sreedhar et al. 1993; Ananthkumar et al. 1994; Subramoniam et al. 1997; Singh and Mukerjee, 1998; Noumi et al. 1998; Sureshkumar et al. 2000), isolated constituents (Waddell and Ibach, 1989) and synthetic compounds (Dallo et al. 1986). Bombax ceiba is reported to possess antihypertensive, antioxidant, antidiabetic, aphrodisiac and uterine tonicity properties (Kirtikar & Basu, 1994; Anonymous, 2001). B. ceiba is also claim (Anonymous, 2003)to use in the treatment of diarrhea, dysentery, menorrhagia, styptic and for wounds. These claims are based largely on subjective opinion rather than scientific observation. The present study was undertaken to investigate the aphrodisiac activity of B. ceiba at doses of 400 mg/kg body wt. in male mice.

Materials and Methods
Plant material and preparation of extracts

Roots of Bombax ceiba were collected from Govt. Vidharbha Institute of Science & Humanities localities, Amravati (Maharashtra). The plant was identified and authenticated by Dr. Vishal R. Marathe of Department of Botany, Shri Shivaji Science College, Amravati and dried in the shade at room temperature. Dried roots were powdered in grinder and powder material (100 g) was suspended in 1 liter mixture of ethanol: distilled water (70:30). The suspension was stirred at 40 °C for 24 h and heated at 50 °C for 2 h. The extract was filtered then and dried (2.4 % w/w yield).

Animals
Adult Swiss albino mice (weight – 25 to 35 gm) of either sex were used for the study. The animals were fed with standard animal feed and water ad libitum. The animals were housed at a temperature of 25 ± 1 0C with a reversed light dark cycle (light from 2300 h to 1100 h) and relative humidity of 45–55 %. The study was performed as per the protocols and recommendation of the Institutional Animal Ethics Committee, Govt. College of Pharmacy, Amravati.

Male mice (n = 5/group) were trained for sexual experience. To provide sexual experience, each male mice was allowed 30 min exposure to a female mice (used as mating stimulus) in behavioral estrous, several days before testing for copulatory performance in a transparent arena. The animal were tested 3 times over a 10 day period for copulatory behavior and divided into active and inactive groups. Sexually active animals were divided into control (saline), Chlorophytum boriviliannum treated (400 mg/kg body wt.) and Bombax ceiba treated (400 mg/kg body wt.) groups. The animals that did not show any sexual interest during training were considered the inactive group that was administrated Bombax ceiba (400 mg/kg body wt.).

Female mice were artificially brought into estrous by the administration of a single subcutaneous dose of 2 µg/kg body wt. of estrogen benzoate and 500 µg/kg body wt. progesterone 48 h and 6 h before the copulatory study (S. Ramachandran et al.2004).

Sexual behavior study
The following guidelines (Hart et al. 1983; Islam et al. 1991) were followed in the study: a) males were kept individually but females were kept in groups; b) training of each male for 15 min at a time was performed until sexual behavior was elicited and when the behavior was noticed, males were exposed to receptive females (1 male with 5 females); c) repeated training to overcome the lack of sexual response in the presence of observers; d) the study was conducted in a silent room under dim red light; e) any jerking movement of the mating area was avoided to enable the mice to chase each other; and g) cleaning of the mating area was performed after each trial, since the urine trails left by one mice might alter the sexual behavior of the next mice.

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