REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY: AN EFFECTIVE TOOL FOR DRUG ESTIMATION
About Authors:
Bhawana Kapoor*1, Vishnukant Rai2, Sonu Sharma3
1Seth G.L. Bihani S.D. College of Technical Education, Sriganganagar, Rajasthan, India
2Shri Ramnath Singh College of Pharmacy, Gormi, Bhind, M.P., India
3NIMS Institute of Pharmacy, Jaipur, Rajasthan, India
ABSTRACT
Chromatography, although primarily a separation technique, is mostly employed in chemical analysis in which High-performance liquid chromatography (HPLC) is an extremely versatile technique where analytes are separated by passage through a column packed with micrometer-sized particles. Now a day reversed-phase chromatography is the most commonly used separation technique in HPLC. The reasons for this include the simplicity, versatility, and scope of the reversed-phase method as it is able to handle compounds of a diverse polarity and molecular mass. This review covers the importance of RP-HPLC in analytical method development and their strategies along with brief knowledge of critical chromatographic parameters need to be optimized for an efficient method development.

Optimal environment to natural, personal and professional development leading to the making of responsible citizens. The day begins in the holy and spiritual environment with a prayer. Complete discipline, unfitness and the unity brought to be attained thought a dress code for staff members and students.
The National Institute of Pharmaceutical Education & Research (NIPER) is an Institute of National Importance by an Act of Parliament under the aegis of Department of Pharmaceuticals, Ministry of Chemicals & Fertilizers, Government of India. The institute is located at S.A.S. Nagar, Punjab adjoining Chandigarh the City Beautiful.

