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Pharma Analysis

  • Solvent effect on absorption spectroscopy and Difference between UV and Visible Spectroscopy

    Solvent Effect
    Solvents play an important role in UV spectra. Compound peak could be obscured by the solvent peak. So a most suitable solvent is one that does not itself get absorbed in the region under investigation. A solvent should be transparent in a particular region. A dilute solution of sample is always prepared for analysis. Most commonly used solvents are as follows.

  • Electronic transition level
    Electronic transition level

    It was earlier stated that σ, π, and n electrons are present in molecule and can be excited from the ground state to excited state by the absorption of UV radiation. The various transitions are n→∏*, ∏→∏*, n→σ*, & σ →σ*

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