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  • HPTLC INSTRUMENTATION: AN OVERVIEW

    ABOUT AUTHORS:
    Arshad Hala
    Seth G. L. Bihani S. D. College of Technical Education,
    Institute of Pharmaceutical Sciences & Drug Research,
    Gaganpath, Sri Ganganagar, Rajasthan -335001, India

    ABSTRACT:
    HPTLC is one type of planar chromatography and most advanced form of instrumental TLC. Now a day, HPTLC is more useful than TLC and HPLC. Because HPTLC is independent of sample application, chromatogram development, detection, etc. it is not only instrumental TLC but entire concept that include widely standardize methodology based on validated method. It is instrument controlled by software. In this review article, we discussed about which type of instrument used in HPTLC, complete HPTLC methodology, How HPTLC better than TLC.

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  • VARIOUS DETECTORS USED IN THE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH COMPARISON OF THEIR SENSITIVITY

    About Authors:
    Patel Amit, Hala Arshad, Beniwal Prashant
    Seth G. L. Bihani S.D. College of Technical Education,
    Institute of Pharmaceutical Sciences and Drug Research,
    Sri Ganganagar, Rajasthan, INDIA

    ABSTRACT:
    The HPLC detectors are used to detect the solute present in the eluent comes from the HPLC column. There are various types of detectors available, but Detector selection in HPLC system is most valuable part in the solute detection. After comparison of all detector, find out which is the best detector for our solute analysis? Then Detector selection was carried out.  Comparisons of all detectors are done on the basis of the sensitivity of the detector for that particular solute.

  • ELECTRONIC TRANSITION IN UV VISIBLE SPECTROSCOPY

    About Authors:
    Tarun Patel , Prof. Dr. Vipin Kukkar, Nilesh Sovasia
    Seth G.L. Bihani S.D. College of Technical Education,
    Institute of Pharmaceutical Sciences and Drug Research,
    Sri Ganganagar, Rajasthan, INDIA

    INTRODUCTION
    When we speak of a molecule as being raised to a higher electronic level we mean that an electron has been changed from one orbital to another orbital of higher energy. This electron can be of any kinds we have encountered –a σelectron, a π electron or an n electron. In Ultraviolet region we are confined only to the excitation of the comparatively loosely held n and πelectrons.2

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