Biotechnology Articles

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About Author
Nirav Soni
Department of Quality Assurance,
A-One Pharmacy College,
Anasan, Ahmedabad, Gujarat, India
nirav_sonic@yahoo.com

ABSTRACT
Isolation of Nucleic acid easily by Solid Phase Extraction (SPE) and this approach, using commercially available Extraction of nucleic acid column-based kits, requires no toxic chemicals and is a rapid and consistent method for concomitant protein extraction. It is a modern technique useful for separation of Nucleic acid which is  most reliable, less time consuming and separation of  impurities and continuity of reactive products using listed techniques like column-based nucleic acid purification, Nucleic acid methods& ethanol precipitation, DNA separation by silica adsorption. Buffer choice is significant to completely solubilized all proteins in the sample. This technique provides a simple and effective way to analyze protein and nucleic acids simultaneously from the same sample not affecting yield and quality.

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ABOUT AUTHORS
Vivek P. Chavda^1*, Dharmesh Shaliya², Bhargav Patel³, Ashish Gabani^4
1Department of Pharmaceutics, B.K. Mody Government Pharmacy College, Rajkot, Gujarat, India
²Quality Assurance, Intas Pharmaceuticals Ltd., Ahmedabad, Gujarat, India
³Quality Assurance, Zydus cadila pharmaceuticals Ltd., Ahmedabad, Gujarat, India
⁴ Intas Biopharmaceuticals Ltd., Ahmedabad, Gujarat, India
* vivek7chavda@gmail.com

ABSTRACT
The drug delivery systems have made some of the technological advances especially in the case of biopharmaceuticals. When one talks about biotechnological product stability of drug product becomes main culprit due to dynamic nature of drug molecule. This review is grafted using ICH and other regulatory guidelines to provide an overview to stability testing of such molecules.

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About Authors:
Shikha Jain*, Ranjana Joshi, Kirti Jatwa, Avnish Sharma, S.C. Mahajan
Department of Pharmaceutics,
Mahakal Institite of Pharmaceutical Studies,
Behind Air strip, Datana, Dewas Road, Ujjain, M.P.
jain.shikhapharma@gmail.com

Abstract
Microsatellites consist of tandemly repeated sequence, no more than 6 bases long. They are scattered throughout most eukaryotic genomes. The major characteristic that makes microsatellites as useful and powerful genetic tool is the extensive length polymorphism that first of all reflects allelic variation in the number of the tandemly arranged perfect repeats. Microsatellite are generally considered as the most powerful genetic marker.
A genetic marker is a gene or DNA sequence with a known location on a chromosome that can be used to identify individuals or species. Genetic marker that reveal polymorphisms at the DNA level are called molecular marker.
Molecular markers are called as DNA markers, which should be thought of as signs along the DNA trail that pinpoint the location of desirable genetic traits or indicate specific genetic differences.They are responsible for various neurological diseases and hence the same cause can now be utilized for the early detection of various diseases, such as, Schizophrenia, Bipolar Disorder and Congenital generalized Hypertrichosis . These agents are widely used for forensic identification and relatedness testing, and are predominant genetic markers in this area of application.

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ABOUT AUTHORS:
Nirav.R. Soni
M.Pharm, A-One Pharmacy College,
Anasan, Ahmedabad-382330, India
nirav_sonic@yahoo.com

ABSTRACT:
Pharmaceutical Biotechnology is not a source of energy, but a scientific method that provides tools to produce energy based entirely on modern biotechnological techniques, as to date encompass a wider range of altogether newer medicinal compounds, e.g., antibiotics, vaccines development and monoclonal antibodies (MABs) that may now be produced commercially using well-defined, optimized and improved fermentative methodologies. In fact, genetic engineering has brought in a sea change by virtue of the directed construction of microorganisms resulting in a plethora of newer life-saving drugs. Focus on a variety of  Research areas including health/medicine, food science, environmental science and agri- science and also bioenergy development.

ABOUT AUTHOR:
Muhammed Mujahed
Master’s of Science in Biotechnology.
SRTM University.
mujubiotech2011@rediffmail.com

INTRODUCTION:
Drug design is an integrated developing discipline which portends an era of ‘tailored drug’. It involves the study of effects of biologically active compounds on the basis of molecular interactions in terms of molecular structure or its physico-chemical properties involved. It studies the processes by which the drug produce their effects, how they react with the protoplasm to elicit a particular pharmacological effect or response how they are modified or detoxified, metabolized or eliminated by the organism.

Disposition of drugs in individual region of biosystems is one of the main factors determining the place , mode and intensity of their action . The biological activity may be “positive” as in drug design or “negative” as in toxicology. Thus drug design involves either total innovation of lead  or an optimization of already available lead. These concepts are the building stones up on which the edifice of drug design is built up.

The drug is most commonly an organicsmall  molecule that activates or inhibits the function of a biomolecule such as a protein, which in turn results in a therapeutic benefit to the patient. In the most basic sense, drug design involves the design of small molecules that are complementary in shape and charge to the biomolecular target with which they interact and therefore will bind to it. Drug design frequently but not necessarily relies on computer modeling techniques. This type of modeling is often referred to as computer-aided drug design. Finally, drug design that relies on the knowledge of the three-dimensional structure of the biomolecular target is known as structure-based drug design.