• 30 May 2012

    VALIDATION OF UV SPECTROPHOTOMETRIC METHOD WITH STRESS DEGRADATION STUDY FOR METFORMIN HYDROCHLORIDE

    About Authors:
    *Shrikrishna Baokar, Vinod Pawar, Smrutidevi Sonavane, Mahesh Rode, Deepali Kate
    Shivnagar Vidya Prasarak Mandal’s College of Pharmacy,
    Malegaon (Bk II), Tal-Baramati,
    Dist- Pune, Maharashtra, India- 413115
    *krishnabaokar@gmail.com

    ABSTRACT
    A Simple, sensitive, specific, spectrophotometric method has been developed for detection of Metformine Hydrochloride in pure and pharmaceutical formulations. The optimum condition for the analysis of the drug was established. Metformine exhibits absorption at 228 nm and obeyed beers law in the concentration range 02 to 10 ppm. The correlation coefficient was found 0.9988 and slope of line 0.1148. The %R.S.D. for intra assay precision of the method was found 1.08 whereas Inter assay precision was found to be 0.4433 (Average). The sample solution was stable up to 24 hours. The assay results were found to be in good agreement with label claim. The degradation study was checked at different conditions like with acid, alkali, dry heat, oxidative and photolytic degradation. All the results found in degradation study were satisfactory. So this proposed method was simple sensitive, precise, quick and useful for routine quality control.

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