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METHOD DEVELOPMENT AND ITS VALIDATION FOR ESTIMATION OF TORSEMIDE IN TABLET DOSAGE FORM

About Authors:
Kapil Sharma1*, Subhash Gupta 2, Yogesh Sharma1
1 Yaresun Pharmaceuticals Pvt. Ltd.Jaipur - 302006, Rajasthan, India.
2 Oasis test house ltd.jaipur-302006,
Rajasthan, India.

METHOD DEVELOPMENT AND ITS VALIDATION FOR ESTIMATION OF TORSEMIDE IN TABLET DOSAGE FORM BY RP-HPLC AND UV SPECTROPHOTOMETRY AND COMPARISON OF TWO DEVELOPED METHODS BY USING t-TEST

ABSTRACT
One HPLC and one UV spectrophotometric method have been developed for the determination of torsemide (TRS) in tablet dosage form. The first method is based on determinetion of TRS in tablet dosage form by RP- HPLC method.  Chromatgraphy was carried out on a nucleosil C-18,250 x 4.6 mm column using a mixture of phosphate buffer and methanol  (50:50 v/v)  as the mobile phase at a flow rate of 1.3 ml/min.  Run time was 15 min.  Detection was done at 288 nm and retention time of the drug was 7.05 min.  This method produced linear responses in the concentration range 60-140  µg/ml of torsemide. The accuracy of the method was assessed by recovery studies and was found to be 99.90± 0.41 for torsemide.  The second method is based on the estimation of torsemide in tablet dosage form by UV spectrophotometry using 50% v/v methanol in distilled water.  Beer’s law obeyed over the concentration range 2-26 µg/ml at 288 nm with apparent molar absorptivity of 1.26 x 104.  Both developed methods were found to be applicable for routine analysis of drug in tablet dosage form. The result of the analysis were validated statistically.The results were compared obtained from UV spectrophotometry and HPLC.

Microsphere: An Overview

About Author:
Rajesh Mujoriya
Sardar patel college of technology,
Balaghat, dis. Balaghat,
MP–481001,INDIA

Abstract
Microspheres offer an excellent solution to creating precise pore sizes in ceramics at reasonable prices.  Polyethylene microspheres offer the added benefit of minimal residue after firing, and the availability in wide size ranges from a few micron up to 1000um (1mm).    Highly spherical microspheres have the added benefit of creating strong pores without any stress risers. Biodegradable microspheres are used to control drug release rates thereby decreasing toxic side effects, and eliminating the inconvenience of repeated injections. Microparticulate carrier system can be administered through different routes such as i.v,ocular,i.m,oral,intra arterial.etc.Each routes has it’s own biological significance, limitation & pharmaceutical feasibility.

Openings for Project Fellow and Lab attendent in Department of Pharmacy @ Banaras Hindu University

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Walk in for the post of Senior Project Fellow at INSTITUTE OF HIMALAYAN BIORESOURCE TECHNOLOGY

Govt. of Himachal Pradesh took up initiative in early seventies to set up a CSIR lab to make prudential use of the natural resources of the region. After several levels of discussions a formal request was made by then Chief Minister of HP to Vice President, CSIR in 1982 for initiating the matter, and giving final shape to the proposal.

Applications are invited for the post of Project Fellow in a project sanctioned by Defence Research Laboratory @ BITs

BIT, Mesra is a "Deemed University" under Sec. 3 of the U.G.C. Act 1956.

Work as Manager/ Sr. Manager-QA -Audit and Plant Compliances in Aurobindo Pharma

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Review on: THE PHARMACEUTICAL PACKAGING

About Authors:
Manoj,
Shekhawati College of Pharmacy,
Dundlod

1) Introduction:
Packaging can be defined as an economical means of providing presentation, protection, identification information, containment, convenience and compliance for a product during storage, carriage, display and until the product is consumed. Packaging must provide protection against climatic conditions biological, physical and chemical hazards and must be economical. The package must ensure adequate stability of the product throughout the shelf life.1

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ISCHAEMIC HEART DISEASE: AN OVERVIEW TO HEART DISEASE

About Author:
Rajesh Mujoriya
Sardar patel college of technology,
Balaghat, dis. Balaghat,
MP–481001,INDIA

Abstract
Ischaemic Heart Disease is a condition that affects the supply of blood to the heart. The blood vessels are blocked due to the deposition of cholesterol plaques on their walls. This reduces the supply of oxygen  and  nutrients  to the  heart musculature, which is essential for proper functioning of the heart. This may eventually result in a portion of the heart being suddenly deprived of its blood supply leading to the death of that area of heart tissue, resulting in heart attack.  
 In 1963 the Ministry of Railways carried out a survey with a view to ascertaining the number of deaths due to ischimic heart disease among railway populations in different parts of the country. The method employed was to obtain data from all the railway zones on a proforma based on W.H.O. classification 420, for arteriosclerotic, including coronary heart disease.
The epidemiology studies have provided several key points of information related to the risk of developing IHD. First, several specific risk factors for IHD have been identified. Second, evidence that these factors are closely related to environmental and life-style changes implies that risk factors are potentially alterable. Third, these studies have stimulated further consideration and investigation of the basic mechanism of atherosclerosis. Angiographic studies have indicated a direct relationship between the risk factors and the severity of coronary disease.

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