Method development and validation for estimation of Naproxen in bulk samples as well as in tablet dosage forms by using RP-HPLC
PharmaTutor (September - 2016)
Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 4, Issue 9)
Received On: 17/03/2016; Accepted On: 19/04/2016; Published On: 01/09/2016
S. Ashutosh Kumar*, Manidipa Debnath, Vaddi Pavan Krishna Kumar
Department of Pharmaceutical Analysis and Quality Assurance,
A.K.R.G College of Pharmacy, West Godavari, A.P, India
ABSTRACT: Purpose: A simple, precise, accurate, rapid and economical reverse phase high-pressure liquid chromatographic method has been developed as per ICH norms for the estimation of Naproxen from pharmaceutical formulation.
Methods: The method was carried out on a Kromosil-C18 ODS column (150 mm X 4.6 mm; 5 μ) with a mobile phase consists of ammonium acetate buffer (adjusted to pH 4.0 with 1 % Triethyl amine): methanol (40:60 v/v) and filtered through a 0.45 μ cellulose nitrate filters. The flow rate was maintained in isocratic mode at 1.0 mL/min. The detection was carried out at 210 nm. The run time was 7.0 min.
Results: The retention time was 3.063 min for Naproxen. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification and solution stability.
Conclusion: The proposed method was adequate sensitive, reproducible, and specific for the determination of Naproxen in bulk as well as in tablet dosage forms.
How to cite this article: Kumar SA, Debnath M, Kumar VPK; Method development and validation for estimation of Naproxen in bulk samples as well as in tablet dosage forms by using RP-HPLC; PharmaTutor; 2016; 4(9); 33-39