Articles

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ABOUT AUTHORS:
Sagar Alone*, Sharda Deore, Bhushan Bawiskar
Department of Pharmacognosy & Phytochemistry
Govt. College of pharmacy, Kathora naka, Amravati-444604, India
*sagaralone123@gmail.com

ABSTRACT
Total seventeen saponins previously isolated from roots of Pulsatilla koreana having cytotoxic activity against 4 different cancer cell line (A-549, SK-OV-3, SK-MEL-2, HCT15) were used for 2D QSAR using V-life Molecular design suit. Using multiple linear regression method against 4 different cell lines develops QSAR model. QSAR model was generated by using training set of 11 and test set of 6 molecules having correlation coefficient (r²), significant cross validated correlation coefficient (q²) and F-test (For statistical significance) is as given below  (A-549: r²- 0.9281, q²- 0.8691, F-test- 51.6079),  (SK-OV-3: r²- 0.9554, q²- 0.9184, F-test- 85.7357),  (SK-MEL-2: r²- 0.9160, q²- 0.8285, F-test- 43.6084),  (HCT15: r²- 0.9203, q²- 0.8357, F-test- 46.1887). In this QSAR study Alignment independent descriptors such as T_2_C_7, T_O_O_5 and physicochemical descriptors like Chain path count such as 6 chain count and Chi chain such as Chi 6 chain were most responsible descriptors for anticancer activity.

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ABOUT AUTHORS:
Somsubhra Ghosh¹*, B. V. V. Ravikumar², B. Mahanti^1
1Bharat Technology, Banitabla, Uluberia, West Bengal
²Roland Institute of Pharmaceutical Sciences, Berhampur, Odisha
som_subhra_ghosh@yahoo.co.in

ABSTRACT
Validation is a very important tool in GMP. Main aim & objective of GMP to all Pharmaceutical agencies are to provide a good & reasonable quality of Pharmaceutical products to people. To get that desired quality Validation is great support to all Pharmaceutical & other industry people. Validation is also a very important tool to save money, time, labourer, waste material etc. There are different types of Validation used all over the world by which we can achieve our goal very easily. In this review it is briefly described scope, importance, objectives & types of Validation as per international norms. Now days it became so important that without validation any process, method or instrument are not accepted globally.

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ABOUT AUTHROS:
Vandana M Patel*, Hasumati A Raj, Vineet C Jain
*Shree Dhanvantary College of Pharmacy,
Kim, Surat, Gujarat, India.
vandanapatel@gmail.com

ABSTRACT
A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Amlodipine besylate and Simvastatin in synthetic mixture using Absorbance correction  method. At  360.80  nm (λmax of Amlodipine besylate) Simvastatin has zero absorbance so Amlodipine besylate is directly estimate at 360.80 nm. At 237.60 nm (λmax of Simvastatin) both drugs have some absorbance so Simvastatin is estimate at 237.60 nm using absorbance correction method. The method was found to be linear (r2>0.999) in the range of 5-10 μg/ml for Amlodipine besylate at 360.80 nm. The linear correlation was obtained (r2>0.999) in the range of 5-10 μg/ml for Simvastatin at 237.60 nm. The limit of determination was 0.17 μg/ml and 0.10μg/ml for Amlodipine besylate and Simvastatin, respectively. The limit of quantification was 0. 54μg/ml and 0. 32μg/ml for Amlodipine besylate  and Simvastatin, respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99%. The method was successfully applied for simultaneous determination of Amlodipine besylate and Simvastatin in binary mixture.

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ABOUT AUTHORS:
Fatema Nasrin¹*, Nabila Mahrin², Nisrat Jahan¹, Yesmin Begum¹, Senjuti Majumder^1
1Department of Pharmacy, Southeast University, Banani, Dhaka
²Pharmacology labortory, Department of Pharmacy, Southeast University, Banani, Dhaka
nasrin_0209@yahoo.com

ABSTRACT
We aimed at assessing the effect of methanolic extract of Streblus asper in human red blood cell (HRBC) membrane stabilization and insecticidal (on the stored grain pest, Trogoderma  granarium Everts) as studies. The membrane stabilizing activity was assessed by using erythrocyte in hypotonic solution and heat induced was compared with acetyl salicylic acid. The extract at the doses of  200, 400 and 800 μg/ml significantly inhibited heat induced lysis of the human red blood cell membrane with values of 46.53%, 56.52% and 65.14%, respectively. The results of hypotonic solution induced lysis showed that S. asper has significant reduction (P≤0.01) in inflammation i.e. 40.8 % (400 µg/ml) and 50.8 % (800 µg/ml) as compared to the standard drug, acetyl salicylic acid, which was 62.96 % in insecticidal assay the extract showed dose dependent paralyzing effect and mortality of T.  granarium Everts. All the doses of crude extracts exhibited concentration and time dependent insecticidal activity.

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ABOUT AUTHORS:
Suresh VV Babu¹, Talasila EGK Murthy²*, Chimakurthy Jithendra^3
1Dept. of Pharmaceutics, Natco Pharma Limited, Hyderabad, Telangana, India.
²Dept. of Pharmaceutics, Bapatla College of Pharmacy, Bapatla, Andhra Pradesh, India.
³Dept. of Pharmacology, Bapatla College of Pharmacy, Bapatla, Andhra Pradesh, India.
*drgkm@bcop.net

ABSTRACT
The present study was to assess the relative bioavailability and pharmacokinetic properties of extended release formulations of Ranolazine 1000 mg in healthy male volunteers usinga randomized, open-label, balanced, two-treatment, two-period, two sequence, single dose, crossover, bioequivalence study under fasting conditions. Bioavailability of the test product of Ranolazine extended release tablets 1000 mg was compared with that of the reference product of Ranexa® (Ranolazine extended release tablets 1000mg) of CV Therapeutics Inc., California. The plasma samples were collected at 0.50, 1.00, 1.50, 2.00, 2.50, 3.00, 3.50, 4.00, 4.50, 5.00, 5.50, 6.00, 6.50, 7.00, 8.00, 10.00, 12.00, 16.00, 24.00 and 48.00 hours post dose after single administration of Ranolazine 1000mg. The plasma Ranolazine concentrations were estimated by using a validated bioanalytical method by LC-MS/MS. A ten day washout period is followed between two treatments. The formulations were considered to be bioequivalent if the 90% CIs for the log-transformed values were within the predetermined equivalence range 80%–125% for AUC and C_max. For Ranolazine, at 90% confidence intervals C_max, AUC_0-tand AUC _0-∞ were 83.43-113.29, 82.10-102.87 and 80.94-101.85 for log-transformed data respectively.The present results show that the formulation of Ranolazine was bioequivalent to the reference in fasting, healthy, male volunteers.

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ABOUT AUTHORS:
Rajmahamad H. Shaikh¹*, Mohsin J. Jamadar¹, Amol D. Patil¹, Audumbar D. Mali², Sanauaha M.Tamboli^1
1Department of Pharmaceutics, Appasaheb Birnale college of Pharmacy, Sangali, Maharashtra, India.
²Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, India
rajshaikh71@gmail.com

ABSTRACT:
The purpose of this investigation was to enhancement of solubility of cinnarizine by using solid dispersion technique solvent evaporation method using polymer PEG 6000 & develop combination ODT of cinnarizine with domperidone by using direct compression technique using crospovidone, croscarmellose sodium and sodium starch glycolateas a superdisintegrants. The preformulation study includes the compatability of drugs with the polymers by using FTIR,UV,TLC. The batches were evaluated for weight variation, hardness, friability, drug content, wetting time, IN In-vitro dispersion, in-vitro dissolution. The formulation F2 which contain 8% crospovidone and 10 % sodium starch glycolate showed best results and rapid in-vitro dissolution. The results revealed that the tablets containing superdisintegrants combination had a good dissolution profile. The drug content of all the batches was within the acceptable limits of the United States Pharmacopoeia with maximum drug being released at all timeintervals. The present study demonstrated potentials for rapidabsorption, improved bioavailability, effective therapy and patientcompliance. The results conclusively demonstrate successful enhancement of solubility, disintegration and dissolution of the formulated tablets.

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ABOUT AUTHORS:
Shete Reshma S.¹*, Gadhave Manoj V.², Gaikwad D. D.^3
1Department of Quality Assurance Techniques,
²Department of Pharmaceutics,
³Department of Pharmaceutics,
VJSM’S Vishal institute of pharmaceutical education and research, Ale, Pune, Maharashtra, 412411
*reshma.s.shete@gmail.com

ABSTRCT
Simvastatin is a poorly soluble drug exhibiting poor dissolution pattern. Simvastatin, PEG 6000 & Poloxamer 407 solid dispersions were prepared with a view to study the influence of polymer on solubility and dissolution of this poorly soluble drug Simvastatin. Solid dispersions of Simvastatin were prepared using different ratios of PEG 6000 & Poloxamer 407 as carrier by, solvent evaporation method. They were evaluated for percentage yield, drug content, FTIR spectral studies, DSC, XRD, solubility, and in-vitro dissolution. The solubility profile indicated that there is increase in solubility of Simvastatin when polymer concentration is increased. The solid dispersion complex of drug (1:5:5 ratios) was giving better dissolution profile as compared to pure drug and other solid dispersions. This in turn can improve the bioavailability. FT-IR, DSC shows the compatibility of drug and carrier.

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ABOUT AUTHOR:
Mohammad Asif
Department of Pharmacy, GRD(PG) Institute of Management & Technology,
Dehradun, (Uttarakhand), 248009, India
aasif321@gmail.com

ABSTRACT:
The reappearance of tuberculosis and the rush of multidrug-resistant clinical isolates of Mycobacterium tuberculosis have reaffirmed tuberculosis as a key public health concern. Describe findings on the pharmacological status of Benzothiazinones as new agents that are being developed as antitubercular drugs. Benzothiazinones act by targeting the enzymes responsible for the formation of arabinans that are essential parts of the cell wall. In view of their novel mechanism of action, these drugs appear promising as anti-TB drugs and considered to be promising candidates for future development.