Estimation of Ondansetron Hydrochloride in bulk and formulation by second order derivative area under curve UV-Spectrophotometric Methods
PharmaTutor (August- 2015)
Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 3, Issue 8)
Received On: 03/04/2015; Accepted On: 10/04/2015; Published On: 01/08/2015
AUTHORS: Jadhav Santosh1*, Kharat Rekha1, Ansari Afaque2, Tamboli Ashpak3
1Department of Pharmaceutics, Sahyadri College of Pharmacy, Methwade, Sangola-413307, Solapur, Maharashtra, India.
2Department of Pharmaceutic’s, D. S. T. S Mandal’s College Of Pharmacy Solapur, Maharashtra India.
3Department of Pharmaceutical chemistry, Sahyadri College of Pharmacy, Methwade,
Sangola-413307, Solapur, Maharashtra, India.
ABSTRACT: Simple, fast and reliable spectrophotometric methods were developed for determination of Ondansetron Hydrochloride in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Distilled Water. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 248-254nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ondansetron Hydrochloride using 2-10μg/ml (r²=0.9986) for second order Derivative Area under Curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ondansetron Hydrochloride in pharmaceutical formulations.
How to cite this article: S Jadhav, R Kharat, A Ansari, A Tamboli; Estimation of Ondansetron Hydrochloride in bulk and formulation by second order derivative area under curve UV-Spectrophotometric Methods; PharmaTutor; 2015; 3(8); 42-46