Articles

About Authors:
CHIRAG S. RAJPARA*¹, JAWED AKHTAR¹, AMIT KHANDHAR²
1. Department of Quality Assurance, School of Pharmaceutical Sciences, Jaipur National University, Jagatpura, Jaipur-302025, Rajasthan, India.
2. Zydus cadila pharma, moraiya,ahmedabad.
*chiragrajpara2601@gmail.com

ABSTRACT
* A simple, specific, accurate and stability-indicating reversed phase high performance liquid chromatographic method was developed for simultaneous estimation of Tadalafil and Dapoxetine HCL, Water Symmetry C-18 (150 x 4.6 mm),5 µ and a mobile phase composed of Buffer : Acetonitrile (65:35)

* The retention time of Tadalafil and Dapoxetine HCL were found to be10.08 and 4.45 min respectively. Linearity was established for Tadalafil and Dapoxetine HCL in the range of 8.0-60 μg/ml and 24.0-180.0 μg/ml respectively. The percentage recovery of Tadalafil and Dapoxetine HCL were found to be in the range of 99.7-100.6% and 98.07-99.09% respectively. The drug was subjected to acid and alkali hydrolysis, oxidation, dry heat and photolytic degradation. The degradation studies indicated, Tadalafil showed degradation in acid and alkali while it was found stable in H₂O_2, photolytic and in presence of dry heat and Dapoxetine showed degradation in thermal and peroxide while it was found stable in rest of parameters . The degradation products of Tadalafil and Dapoxetine HCL in acidic, alkaline conditions were well resolved from the pure drug with significant differences in their retention time values. This method can be successfully employed for the quantitative analysis of Tadalafil and Dapoxetine HCL in bulk drugs and formulations.

About Authors:
Vaibhav Patel*, Punit Bhatnagar, Gopal Rai, Alok Pal Jain
Guru Ramdas Khalsa Institute of Science & Technology (Pharmacy),
Jabalpur
*vaibhavpatel281@gmail.com

Introduction
Gene therapy by small interfering RNAs (siRNAs) hasbeen emerging as innovative nucleic acid medicines with increasing knowledge on the molecular mechanisms of endogenous RNA interference. Gene silencingis a promising tool for the treatment of numerous human diseases that cannot be cured by rational therapies. The primary success of siRNA applications depends on suitable vectors to deliver therapeutic genes.

About Authors:
Roshni Patel*, Prof. Arun Parikh, Prof. Vijayalakshmi  Gudaparthi
Department of Pharmaceutical Chemistry, L.J.Institute of Pharmacy,
S.G.Highway, Ahmedabad-382210
*roshanimpharm@gmail.com

ABSTRACT
Medicinal chemistry involves chemical aspects of identification, and then systematic, thorough synthetic alteration of new chemical entities to make them suitable for therapeutic use. Indazoles have an important role in medicinal chemistry. Indazole  nucleus is an important class of nitrogen containing heterocyclic widely used as key building block for the synthesis of various pharmaceutically important agents. Indazole possess wide range of biological activities such as bactericidal, fungicidal, analgesic, antihypertensive, anti-inflammatory and antitumor. In the present study we have synthesized some novel 3,3a,4,5-tetrahydro-2-(substituted benzenesulphonyl)-3-(substituted phenyl)-2H-benzo[g]indazoles, by condensation of  1-tetralone with different substituted aromatic aldehydes and the resulted 2-(substituted benzylidene)-3,4,-dihydronaphtalen-1-ones on reaction with hydrazine hydrate in methanol followed by treatment with different substituted sulfonyl chlorides in pyridine gave the titled compounds.The synthesized compounds were characterized by IR, NMR and Mass spectral analysis. All newly synthesized derivatives were evaluated for antibacterial activity against  gram + ve and gram-ve bacteria B.cereus, S.aureus and E.coli respectivelyand antifungal activity againstC.albicans and all the synthesized compounds showed significant antibacterial and antifungal activity.

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About Authors:
Gupta S^*, Singal A
Apex Institute Of Pharmaceutical Sciences,
Jaipur, Raj., India
*somesh.gupta88@gmail.com

Abstract
The aim of the present study was to correlate the occurrence of hypertension in the diabetics’ patient by the survey report on hypertension associated with diabetics’ patient. The effect of this study is symbolize that 70% patient have diabetes with hypertension and 30% patient with hypertension only so we can say that in this survey as the BGL increased leads to increased in B.P. And it was also found to be that aged patients have more risk of diabetes. Over 60- 70 year age group patient have 40% risk to get diabetes.

About author:
Ashish Vishwakarma
Department of Pharmaceutics,
Chandigarh College of Pharmacy,
Mohali-140307
ashish1931@gmail.com

ABSTRACT-
Acceptability of any drug dosage form mainly depends upon its taste i.e. mouth feel. In the formulation of   oro-dispersible tablet of bitter drug, the main challenge is to mask the bitter taste of drug, because the drug is dispersed and released in mouth. This is more essential in the formulation for pediatric and geriatric, bed ribbon and non-cooperative patients. There are many methods of taste masking but the main objective of this article is to explore method, technology and evaluation to mask taste of bitter drug by ion-exchange resins. Here we will study the formulation and evaluation of various oro-dispersible tablets of bitter drug. This is more economical, easiest and efficient method for taste masking.

About Authors:
Abdulrhman A. Akasha
Department of Pharmaceutics, Faculty of Pharmacy,
Tripoli University, Libya-13645
akashaabdu@yahoo.co.uk

Abstract
Bioerodible microspheres may provide a vehicle for achieving good aerosolisation characteristics, whilst offering a slow release depot from the pulmonary deposited fraction.  Polysebasic anhydride (PSA) was synthesised by a melt-polycondensation process using nitrogen gas under high vacuum. The products were characterised by ¹H-NMR (2 & 20 KDa) and DSC (melting point: 68 & 83 ^°C), and with a yield (81 & 89 %) of prepolymer and polymer respectively. The polyanhydride was converted into microspheres using an oil in oil emulsification procedure.  The polymer was dissolved in dichloromethane and using 1 to 5% Span 85 or oleic acid as stabilizer, dispersed into silicone oil. The dichloromethane was removed by evaporation, the microspheres recovered and washed with petroleum ether. Laser diffraction analysis of the microspheres indicated a mean microspheres size <5 mm.  The presence of rough non-porous spheres was demonstrated by SEM.  Initial in vitro experiments were undertaken to examine the degradation rate in 0.1 M phosphate buffer at 37 ^°C, pH 7.4.  The process was followed for 24 hr, by which time 73% of the microsphere mass had eroded. However release of salbutamol base occurred more rapidly with 95% lost within 6-10 hr. The fact that 74% was released in the first 10 min suggest a high fraction of surface adsorbed drug. Microcarrier systems for slow release may provide a vehicle for achieving good aerosolisation characteristics, whilst offering a slow release depot from the pulmonary deposited fraction. The produced rough and non-porous microspheres containing salbutamol base were subjected for Deposition process using Andersen Mark II Cascade Impactor. The Deposition was assessed from both a Spinhaler and Rotahaler after 5s activation at a flow rate of 28.3L/min.  This resulted in an average of 33.5% (Rotahaler and 17.5% (Spinhaler) of the dose found between stages 2 and the filter corresponding to microspheres in the size range 4.7 to 0.4 mm.

About Authors:
Borse V. G.*, Birajdar A. S., Datal A. A.
K. T. Patil College of Pharmacy,
Osmanabad-413501, Maharashtra, India.
*vishalgborse@gmail.com

ABSTRACT :
The objective of this study is to enhance dissolution rate of practically insoluble drug olanzapine, using surface solid dispersion technique. Olanzapine is classified as a thiobenzodiazepine atypical antipsychotic drug used in the treatment of schizophrenia. Surface solid dispersion technique is used to prepare solid dispersion of olanzapine using Crospovidone as carrier. Surface solid dispersion were characterised by saturation solubility studies, drug content and in- vitro dissolution studies. Solubility studies revealed a marked increase in the solubility of olanzapine with an increase in Crospovidone concentration. The best dispersion was selected based on saturation study and release study and evaluated for solid state characterization techniques Differential scanning calorimetry, FT-IR spectroscopy study. The results from DSC and IR indicated that there was no interaction between drug and carriers. Surface solid dispersion of olanzapine shows increase in aqueous solubility 4.42 fold than pure drug. SSD with Crospovidone in 1:9 ratio give highest drug release i.e. 88.87%.Surface solid dispersion is successful technique to improved drug dissolution of olanzapine with the carrier Crospovidone.

About Authors:
Vivek Chourasia*, Hemant Nagar, H.S. Chandel, Anindya Goswami
Truba Institute of Pharmacy,
Karond Gandhinagar Bypass
Bhopal (M.P.)-India
*vickyc.chourasia@gmail.com

ABSTRACT
Ficus religiosawas investigated for its possible protective effect against paracetamol and CCl₄-induced hepatic damage. IV administration of a sub-lethal dose of paracetamol (500 mg/kg) produced liver damage in rats as manifested by the rise in serum level of transaminases (AST and ALT). Ttreatment of rats with Ficus religiosa (200 mg/kg) prevented the paracetamol-induced rise in serum enzymes. The hepatotoxic dose of CCl₄ (1.5 ml/kg; orally) also raised the serum AST and ALT levels. The same dose of Ficus religiosa (200 mg/kg) was able to prevent the CCl₄-induced rise in serum enzymes. These results indicate that Ficus religiosa possesses hepatoprotective activity.

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About Author:
Hardik Patel^*, Sagar Solanki
Department of Pharmaceutical Chemistry,
K.B.Raval College of Pharmacy, Shertha,
Gandhinagar-382423, Gujarat, India.
*patel1928@yahoo.in

ABSTRACT
A new, simple, rapid, accurate, precise and sensitive method has been developed for the simultaneous estimation of Furosemide and Spironolactone in their combined tablet dosage form. The method was carried out on a Hiber C₁₈ column (250 mm×4.6mm, i.d.5 μm) with a mobile phase consisting of acetonitrile: water at a flow rate of 1 ml/min and the detection was carried out at 237 nm. The retention time of Furosemide and Spironolactone was 3.81 min and 7.28 min. respectively. Linearity for Furosemide and Spironolactone were found in the range of 2-10 μg/ml and 5-25 μg/ml respectively. The developed method was validated in terms of linearity, accuracy, and precision, limit of detection (LOD) and limit of quantification (LOQ). The proposed method can be used for estimation of both drugs in their combined dosage form.