September 2012

Limited, is a dominant player in the therapeutic areas of cardiovascular (CV) and central nervous system (CNS) and has achieved significant presence in gastro-intestinal, diabetology, anti-infective and pain management segments. Torrent Pharma’s competitive advantage as a manufacturer stems from its world-class manufacturing facilities.

About Authors:
Kapil Sharma^*1, Subhash Gupta ^2
1 Yaresun Pharmaceutical Pvt. Ltd.,India.
² Oasis test house ltd. Jaipur-302006, Rajasthan, India.
*pharma_kapil@rediffmail.com

ABSTRACT
A method for simultaneous estimation of Atorvastatin (ATVS) and Amlodipine (AMLD) in raw material and in combined tablet dosage form has been developed. The method employs the application of RP-HPLC. Chromatgraphy was carried out on a nucleosil C-18,250 x 4.6 mm column using a mixture of phosphate buffer and methanol  (50:50 v/v)  as the mobile phase at a flow rate of 1.3 ml/min.  Run time was 15 min.  Detection was done at 245 nm and retention time of ATVS was 6.97 min and 3.96 min of AMLD.³  This method produced linear responses in the concentration range 20-140 µg/ml and 10-70 µg/ml for ATVS and AMLD respectively. The accuracy of the method was assessed by recovery studies and was found to be 100.54± 0.19 and 100.08±0.80 for ATVS and AMLD respectively.The procedure was successfully applied for the simultaneous determination of both drugs in laboratory prepared mixture of raw material and in market available tablet dosage form. Results of the analysis were validated statistically so that it can be used for routine analysis of ATVS and AMLD in combined tablet dosage form.^4-6

About Author:
Kapil Sharma
Yaresun Pharmaceutical Pvt. Ltd., India.
pharma_kapil@rediffmail.com

Introduction:
Arthritis is the name given for painful joint diseases. Arthritis one of the most common health problems in india. Arthritis also is the leading cause of disability. Rheumatoid arthritis (RA) is a chronicinflammatory condition of the connective tissues throughout the body, but especially around the joints. The main sign of RA is often stiff, painful, and swollen joints.  Areas of typical complaint include the hands, feet, wrists, ankles, and knees.  Depending on the severity of the condition, these areas may eventually become deformed. The exact cause of rheumatoid arthritis remains, largely, unknown.  It has been theorized that a number of genetic and environmental factors may contribute to the disease process, and may include genetics, poor nutrition, lifestyle, chronic stress in rheumatoid arthritis, the joint lining, or synovial membrane, becomes inflamed and the joints become stiff and swollen. The synovial membrane secretes a slippery fluid that covers the cartilage-covered joints and reduces the friction between adjacent joints.The chronic inflammation of rheumatoid arthritis eventually leads to destruction of the cartilage covering the ends of the joints and underlying bone.  In many cases this damage causes joint deformity.

Torrent Pharmaceuticals Limited, is a dominant player in the therapeutic areas of cardiovascular (CV) and central nervous system (CNS) and has achieved significant presence in gastro-intestinal, diabetology, anti-infective and pain management segments. Torrent Pharma’s competitive advantage as a manufacturer stems from its world-class manufacturing facilities.