You are hereMETHOD DEVELOPMENT AND ITS VALIDATION FOR ESTIMATION OF SPIRONOLACTONE IN TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRY

METHOD DEVELOPMENT AND ITS VALIDATION FOR ESTIMATION OF SPIRONOLACTONE IN TABLET DOSAGE FORM BY UV SPECTROPHOTOMETRY


REFERENCES
1. K.D.Tripathi, Essential Medical Pharmacology, 5th edition, Jaypee Brothers, New Delhi, 2004;P. 525 & 537.
2. Indian Pharmacopoeia, 4th ed, New Delhi India, the controller of publications: 1996: Vol-II:709-710.
3. United states pharmacopoeia, 28th ed, Washington, Published by the board of trustees, 2005:Vol-II: 1953.
4. Dinc Erdal, Ustunda Ozgur. Spectrophotometric quantitative resolution of hydrochlorothiazide and spironolactone in tablets by chemometric analysis method. II Farmaco 2003; 58:1151-61.

5. Martin E, Jimenez AI, Hernandez O, Jimenez F,Arias JJ. Simultaneous kinetic spectrometric determination of spironolactone and canrenone in urine using partial least-square regression.Talanta 1999; 49:143-54.
6. De Croo F, Bossche W. vanden, Moerloose P.de. Simultaneous determination of spironolactone and althiazide in tablets by high-performance liquid chromatography. Fresenius’ Journal of Analytical chemistry 2004; 323:91-93.
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Table 1: Recovery study of spironolactone

S.No

Drug

Conc.  before

spiking

(mg/ml)

Reference Std.

added

(mg/ml)

Conc. after

spiking

(mg/ml)

percent

Recovery

Mean

percent

Recovery

1

Aldactone

7.96

4.12

12.16

101.94

100.55

±

1.27

7.96

6.18

14.13

99.83

7.96

8.14

16.09

99.87

Table 2: Summary of validation parameters

PARAMETER

OBSERVATION

Specificity

No interference was found w.r.t. excipients

Linearity

(Correlation coefficient r2)


0.999

Accuracy

(% Recovery)


100.55

Precision RSD

Repeatability (n= 6)

Intra-day (n=3)

Inter-day (days=3)


0.72

0.77

1.14

Figure1:Absorvance of Spironolactone

Figure 2: Calibration curve of spironolactone

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