UV

2 March 2010

Instrumentation of UV or Visible Spectroscopy

INSTRUMENTATION

Instruments for measuring the absorption of U.V. or visible radiation are made up of the following components;
1. Sources (UV and visible)
2. filter or monochromator
3. Sample containers or sample cells
4. Detector

1. Radiation source
It is important that the power of the radiation source does not change abruptly over its wavelength range.
2 March 2010

Solvent effect on absorption spectroscopy and Difference between UV and Visible Spectroscopy

Solvent Effect
Solvents play an important role in UV spectra. Compound peak could be obscured by the solvent peak. So a most suitable solvent is one that does not itself get absorbed in the region under investigation. A solvent should be transparent in a particular region. A dilute solution of sample is always prepared for analysis. Most commonly used solvents are as follows.