About Authors:
Tamal maity
Institute of pharmacy ,(govt.) ,
Jalpaiguri and west bengal university of health sciences
Vidyasagar University
tamalpharma@gmail.com
Abstract:
In an attempt to develop new therapeutics, Vitamin B12 & Folic acid was used to screen the effect on arsenic-induced hepatic toxicity in female rat of Wistar strain. Sub chronic exposure to sodium arsenite (0.4 ppm/100 g body weight/day via drinking water for a period of 28 days) significantly increased activities of hepatic. A notable distortion of hepatocellular histoarchitecture was prominent with a concomitant increase in DNA fragmentation following arsenic exposure. A marked elevation of in hepatic tissue was also evident from the hepatic accumulation of antioxidant enzymes such as superoxide dismutase, Catalase, Xanthine Oxidase, DNA Fragmentation & Histological views. However, co-administration of Vitamin B12 & folic acid (200 µl/100 g body weight/day for a period of 28 days) was found to significantly prevent the arsenic-induced alteration of hepatic function. Moreover, the degeneration of histoarchitecture of liver found in arsenic-treated rats was protected along with partial but definite prevention against DNA fragmentation induction. Similarly, generation of reactive oxygen species and free radicals were found to be significantly less along with restored activities of antioxidant like Vitamin B12 & Folic acid co-administered group with comparison to arsenic alone treatment group. The present investigation offers strong evidence for the hepato-protective and antioxidative efficiencies of Vitamin B12 & Folic acid against oxidative stress induced by arsenic.
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