Modern analytical techniques - Solved Paper 2008-09

M.Pharma

First semester examination, 2008-2009

Modern analytical techniques

Dr A.P.J. Abdul Kalam Technical University

Q1. Attempt any two parts of the following:

(a)  Define and exemplify chromophores. Discuss their interaction with UV- visible radiation and illustrate their use in structural analysis of organic compounds.

(b) Cotton effect curves and octant rule.How would you relate stereo chemical features of a compound with them?

(c)  Write a note on ATR and its application in specific cases.

Q2.  Illustrate any Two of the following:

(a)  Draw line diagrammes and discuss mode of operation of dispersive and interferomatric IR instruments with their merits & demerits.

(b) How would you identify various functional groups by IR spectroscopy provide supporting examples.

(c)  Discuss ELISA assay for antibody by indirect methods and antigen by sandwich method.

Q3. Discuss on any Two of the following:

a) Spin-spin splitting and factors influencing coupling constant.

(b)  Simplification of complex proton NMR spectrum.

(c)  Spectrum editing DEPTspectra and applications.

Q4. Write exhaustively on any two of the following:

(a) Basic fragmentation types, rules and factors influencing it.

(b) Recognition of molecular ion and its role in structure determination.

Q5. Attempt any Two parts of the following:

(a) Write principle difference between conventional chromatography and HPLC. Discuss pumping system, stationary phases and detectors with applications.

(b) Write a note on size exclusion chromatography with its applications.

(c) In NMR spectroscopy, explain shift correlation spectra- COSY and its application in simplifying complex HNMR spectra.

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