Modern analytical techniques - Solved Paper 2007-08

M.Pharma

First semester examination, 2007-2008

Modern analytical techniques

Dr A.P.J. Abdul Kalam Technical University

Q.1. (a) Define and differentiate between auxochromes and chromophores. Exemplify andexplain how auxochrome shifts λmax.

(b) Define fluorescence and phosphorescence. Explain the effect of structural rigidity, temperature and pH on fluorescence?

OR

(a)  Explain Beer’s Law and its limitations in quantitative estimation.

(b)    Give principle of UV-visible spectroscopy. Explain why cellulose is white in colour, picric acid is yellow and aromatic diazotization and coupling produces different shades.

(c) Discuss how certain compounds produce fluorescence or phosphorescence when exposed to electromagnetic radiation. How this property is exhibited only by organic compounds with certain structural specifity? Explain

Q.2. (a) Explain ATR phenomenon and its applications

(b) Show how you would distinguish between the following by spectroscopy.

(i) Aldehyde and Ketone
(ii) Alcohol and Ether
(iii) Mono substituted benzene and Di-substituted benzene

Q.3. (a) Discuss briefly the theory of ESR spectroscopy, spin-spin splitting and its qualitative applications

(b) Explain the significance of magnitude of “J” constant in structure elucidations.

(c) What do you understand by terms “Broad band decoupling” and “off resonance decoupling” in13C NMR? Discuss
Their role in structure elucidation of organic compounds

OR

(a) Illustrate the following with suitable examples

(i)    Diamagnetic anisotrop

(ii)    Correlation Spectroscopy and its significance

(b)    Explain spin labeling & Hyperfine splitting in ESR spectrometry and its application in study of macromolecules.

(c)    Discuss non first order spectra in PMR spectroscopy and its resolution by shift reagents, deuterium labeling and other methods.

Q.4. (a) Discuss the theory of Mass spectroscopy

(b) Explain Index of hydrogen deficiency and its significance.

(c) Write an exhaustive note on DSA technique, its principle and pharmaceutical applications.

Q.5. (a) Discuss various pumping systems, columns & detectors in HPLC.

(b) Write an exhaustive note on size exclusion chromatographic technique and its pharmaceutical applications.

(c) Discuss the principle of separation.

OR

(a) Classify column chromatography techniques.

(b)Discuss the principle of GLC and its column configuration, detection system & stationary phases.

(c)Explain Electrophoresis, its principle and factors governing it.

ABOUT AUTHORS
Mr. Dharmendra Kumar M.Pharm. (Pharmaceutics), Ms. Sakshi Gupta M.Pharm. (Pharmaceutical Chemistry)
Phd(p) Assistant Professor, Dr A.P.J. Abdul Kalam Technical University, Lucknow

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