An Isocratic Method Development and Validation for simultaneous estimation of Rabeprazole and Mosapride in Tablet Dosage Forms by using RP-HPLC
PharmaTutor (July- 2016)
Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 4, Issue 7)
Received On: 27/02/2016; Accepted On: 08/03/2016; Published On: 01/07/2016
S. Ashutosh Kumar*, Manidipa Debnath, Venugopal Padala,
Department of Pharmaceutical Analysis and Quality Assurance,
A.K.R.G College of Pharmacy, Nallajerla, West Godavari, A.P
ABSTRACT: Objective: The present work was undertaken with the aim to develop and validate a rapid and consistent RP-HPLC method in which the peaks will be appear with short period of time as per ICH Guidelines.
Method: The HPLC separation was achieved on an Inertsil-C18 ODS column (250 X 4.6 mm; 5 μ) column in an Isocratic Mode. The mobile phase composed of Methanol [HPLC Grade] (55 %) and Buffer (45 %) [pH 4.0 adjusted with triethylamine]. The flow rate was monitored at 1.0 mL/min. The wavelength was selected for the detection was 276 nm.
Results: The retention times found for rabeprazole and mosapride was 2.946 and 4.186 min respectively. The % recovery was 99.98- 100.03 for rabeprazole and 99.97 - 100.02 for mosapride. The linearity was established in the range of 20-80 μg/mL for both rabeprazole and mosapride. The LOD for rabeprazole and mosapride were 0.01 and 0.035 μg/mL respectively. The LOQ for rabeprazole and mosapride were 0.032and 0.11 μg/mL respectively.
Conclusion: The proposed method was adequate sensitive, reproducible, and specific for the determination of rabeprazole and mosapride in bulk as well as in tablet dosage forms.
How to cite this article: Kumar SA, Debnath M, Padala V; An Iso cratic Method Development and Validation for simultaneous estimation of Rabeprazole and Mosapride in Tablet Dosage Forms by using RP-HPLC; PharmaTutor; 2016; 4(7); 41-51