Antibacterial, Cytotoxic and Antioxidant Activities of n-Hexane, Chloroform and Ethyl Acetate extracts of Trichosanthes cucumerina leaves

  • Posted on: 31 December 2015
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JANUARY 2016 ARTICLE LIST >>

PharmaTutor (January- 2016)

 

Print-ISSN: 2394 - 6679
e-ISSN: 2347 - 7881
(Volume 4, Issue 1)

 

Received On: 02/09/2015; Accepted On: 07/09/2015; Published On: 01/01/2016

 

AUTHOR:
Israt Jahan Bulbul*, Khadiza-tul-Kubra, Mohita Chowdhury Shimu
Department of Pharmacy,
Southeast University Banani, Dhaka, Bangladesh
* israt_jahanb872@yahoo.com

 

ABSTRACT: The main aim of this study was to find out the antibacterial, cytotoxic and antioxidant activities of n-hexane, chloroform and ethyl acetate extracts of T. cucumerina (Cucurbitaceae). Disc diffusion technique was used for in vitro antibacterial screening against gram positive, gram negative human pathogenic bacteria. Here kanamycin disc (30 μg/disc) was used as standard. The chloroform and the n-hexane extract of T. cucumerina showed moderate antibacterial activity with the average zone of inhibition 7-13 mm and 7-9 mm respectively. The brine shrimp lethality bioassay method was used to determine the cytotoxic activity and vincristine sulphate was used as positive control. Among the extractives the chloroform soluble fraction demonstrated the highest cytotoxic activity with LC50 17.09 μg/ml which indicates the compounds present in the chloroform extract are promisingly cytotoxic. Antioxidant activity test of the crude extracts were assessed by means of DPPH free radical scavenging method where ascorbic acid was used as standard. The ethyl acetate fraction of T. cucumerina showed strongest antioxidant activity with IC50 value of 52.18 μg/ml. Incase of phenolic content, the n-hexane, chloroform and ethyl acetate extracts of T. cucumerina revealed 18.79, 31.33 and 29.04 mg of GAE / gm of extractives, respectively.

 

How to cite this article: Bulbul IJ, Kubra KT, Shimu MC; Antibacterial, Cytotoxic and Antioxidant Activities of n-Hexane, Chloroform and Ethyl Acetate extracts of Trichosanthes cucumerina leaves; PharmaTutor; 2016; 4(1); 42-46

 

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