Tilling: Versatile Reverse Genetic Tool

  • Posted on: 15 January 2014
  • By: admin

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PharmaTutor (January- 2014)
ISSN: 2347 - 7881


Received On: 23/12/2013; Accepted On: 27/12/2013; Published On: 15/01/2014


About Authors: Navgeet1, Balraj Singh Gill2, Arvind Negi3*, Shashi Shekhar Anand2
1Institute of Himalayan Bioresource and Technology (CSIR-IHBT), Palampur (HP)
2Centre for Biosciences Central University of Punjab, Bathinda (Punjab)
3Centre for Chemical and Pharmaceutical Sciences, Central University of Punjab, Bathinda (Punjab)


ABSTRACT: Recent years have been witnessed for exhaustive genome sequencing, nourishing a cause to track the mutation and its consequences at the phenotypic level. But reliability of these genomic studies is the critical issue which is still unaddressed and not properly understood. Moreover via TILLING techniques (reverse genetic tool), can be valuable in evaluation of these studies to much extent. Usually TILLING account on a specific gene mutation in order to observe the extent of the functionality of that particular gene at morphological level. This review compiles the literature pertaining to the art of tilling in the evaluation of genomic studies and majorly in concern of functional genomics. Moreover this review also covers the example of the most common species so as to build better understanding of the concept behind this technique of reverse genetics and can be fruitfully applied in extracting the ethano-botanical and therapeutic effect of various medicinal plants.


How to cite this article: A Negi, Navgeet, BS Gill, SS Anand, Tilling: Versatile Reverse Genetic Tool, PharmaTutor, 2014, 2(1), 26-32