Formulation of Doxycycline Loaded Floating Film using Bio-Material Extracted from Tagetes Eracta
PharmaTutor (December- 2013)
ISSN: 2347 - 7881
Received On: 26/11/2013; Accepted On: 03/12/2013; Published On: 20/12/2013
Authors: N.V.Satheesh Madhav, Rohit Singh Negi, Vikas Kumar
DIT-Faculty of Pharmacy,
Dehradun Institute of Technology,
Mussoorie Diversion Road,
Makkawala, P.O. Bhagwantpur,
ABSTRACT: The aim of research work was to isolate novel biopolymer from the seeds of Tagetes eracta and to characterize its physicochemical properties along with the acute toxicity. The isolated polymer was subjected for screening its retardability by using as a bio nano carrier for formulating Doxycycline (model drug) loaded floating films. Tagetes is a genus of 56 species of annual and perennial mostly herbaceous plants in the sunflower family. The genus is native to North and South America, but some species have become naturalized around the world. Tagetes species vary in size from 0.01 to 2.2m tall. They have pinnate green leaves blooms are naturally in golden, orange, yellow and white colors, often with maroon highlights. Tagetes grow well in almost any sort of soil. It contains essentials oils, fatty acids, carotenoids and lutein. Tagetes eracta has long been known for its medicinal use, especially for strengthening the heart, and for treating ailments like headaches, swellings and tooth aches. Tagetes eracta seeds were soaked in water and then washed with chloroform and ethyl acetate. Obtained 100 gm of fine powder was soaked in 100ml boiled water for 24 hours. The mixture was filtered and methanol was added in double. The solution was refrigerated for 24 hours and then centrifuged. Precipitate was collected as biopolymer and was dried. The separated biopolymer was subjected for various physicochemical parameters like color, texture, particle size, solubility, colour changing point. Spectral analysis such as IR spectroscopy was done to check the polymeric nature of biopolymers. Drug– polymer interaction and skin irritancy studies are also done to check the polymer safety.
Doxycycline loaded floating films was formulated using biopolymer Tagetes eracta such as (FT 1 to FT 9) in differentratio (1:2 to 1:18). Then these floating films were subjected for various evaluation parameters as weight uniformity, surface pH, thickness, folding endurance, content uniformity, floating cum mucoadhesive study, in-vitro and ex-vivo drug release and in-vivo bioavailability and stability study. Based on comparison study of t50 and t80 FT4 were considered as the best formulation.
Conclusion was drawn that isolated material from the seeds of Tagetes eracta was devoid of acute toxicity and shows polymeric nature which was conform by spectral studies. Apart from that biopolymer it also inbuilt retardability which was confirmed by formulating floating film.
How to cite this article: Kumar V, Madhav NVS, Negi RS, Formulation of Doxycycline Loaded Floating Film using Bio-Material Extracted from Tagetes Eracta, PharmaTutor, 2013, 1(2), 106-128