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  • PRODUCTION,EXTRACTION AND ASSAY OF ANTIBIOTICS FROM BACTERIAL AND FUNGAL ISOLATES

    About Author: D. Gopi Krishna
    M.Phil
    Tirumala Engineering College
    Hyderabad, Andhra Pradesh

    ABSTRACT:
    Antibiotics are one of the most important commercially exploited secondary metabolites produced by the bacteria and employed in a wide range. Most of the antibiotic producers used today are the soil microbes. Fungal strains and streptomyces members are extensively used in industrial antibiotic production. Bacteria are easy to isolate, culture, maintain and to improve their strains. Microbes are omni present and exist in a competitive environment. Bacillus species being the predominant soil bacteria because of their resistant endospore formation and production of vital antibiotics like bacitracin etc. are always found inhibiting the growth of the other organisms. In the present project a soil bacterium with the antibiotic activity is screened studied for morphological  characters which can provide valuable information about the strain. The inhibitory activity of the organism is checked against some of the important opportunistic microbial flora and inoculated into an appropriate designed media depending on the bacterial requirements, and incubated for 48 hrs at 370C.  The produced compound is extracted by solvent extraction and assayed for its activity. Enhancement in the antibiotic production is studied under various parameters like temperature, pH, carbon source concentration, and Sodium nitrate concentration, which may help in the industrial production. The extracted substance was found effective against the gram positive endospore forming bacilli and gram positive coci. Though a large list of antibiotics are commercially available, the search for the most potential one is still on, and this work may provide some potential information on the antibiotic production and the control of microbial strains.

    Objective: The objective of the present study is production extraction and assay of   antibiotics from bacterial and fungal isolates using soil as source.

  • Pharmacognostic Studies of the Stems of Rumex Hastatus

    About Author:
    Rajput Praveen Kumar*, Gahlot Kavita
    College of Pharmacy,
    Institute of Foreign Trade and Management (IFTM),
    Moradabad (UP)

    Abstract:
    Rumex hastatus is the bushy shrub or undershrub 30-90 cm,high,occurring chiefly on dry rocks and hillsides on dry rocks and hillsides of western Himalayas from Kumaun to Kashmir,at altitudes between 300 and 2400 m . Rootstock woody; leaves 2.5-6.5 cm.long,hastate, thick and fleshy ; flowers pink or green tinged with pink, polygamous ,on terminal panicles;nut small,trigonous.
    Leaves have a pleasant acid tase and can be eaten;they also serve as an ingredienr for chutney and pickles.Root and stem yield 21-23 percent tannin.

  • Lawsonia inermis - From traditional use to scientific assessment

    About Authors: Inder Kumar Makhija
    Department of Pharmacognosy,
    Manipal College of Pharmaceutical Sciences,
    Manipal University, Manipal-576 104,
    Karnataka

    ABSTRACT
    The world is endowed with a rich heritage of medicinal plants. The use of medicinal agents presumably predates the earliest recorded history. Medicinal plants are widely used by traditional practitioners for various ailments. Lawsonia inermis (Lythraceae) commonly known as ‘Henna’is a well-known plant used in the Indian medicine. Various parts of this plant have been used in traditional Indian medicine. The plant has wide range of phytochemicals including lawsone, isoplumbagin lawsoniaside, lalioside, lawsoniaside B, syringinoside, daphneside, daphnorin, agrimonolide 6-O-β-D-glucopyranoside, (+)-syringaresinol O-β-D-glucopyranoside, (+)-pinoresinol di-O-β-D-glucopyranoside, syringaresinol di-O-β-D-glucopyranoside,  isoscutellarin3β, hennadiol, (20S)-3β, 30-dihydroxylupane, lawnermis acid, 3-methyl-nonacosan-1-ol, laxanthones I, II, III and lacoumarin etc. The various in-vitro and in-vivo studies of L. inermis reported the plant to have antibacterial, antifungal, antiparasitic, antiviral, anticancer, antidiabetic, tuberculostatic, anti-inflammatory, antifertility and wound healing properties. This review discusses on the botany, traditional use, phytochemistry and pharmacological data of the plant.

  • AGELESS AGING

    About Authors: NEHA BATRA, S K Dubey
    M.PHARMACY-PHARMACOGNOSY
    JNU-JAIPUR

    ABSTRACT:
    Ageing or aging is the accumulation of changes in an organism or object over time. Ageing in humans refers to a multidimensional process of physical, psychological, and social changes, the things you do this year can halt and even reverse many of health and cognitive issues faced by those over age 50. You could be in better shape, have fewer aches and pains AND have more energy than you did 10 years ago. Anti aging is possible. The body requires two vital materials- energy and raw material for anti-aging. There are many food and herbal treatment available in market for anti aging.

  • STRESS HEALING THEREPY FOR BETTER LIFE

    About Authors: HONEY JAIN, Mr Ranjan Bairwa
    M.PHARM , PHARMACOGNOSY
    JNU
    JAIPUR

    ABSTRACT
    Even with improvements in health care, many Boomers are finding themselves overwhelmed with the stresses of modern life. Luckily, there are natural ways to relieve stress, including daily exercise, meditation, deep-breathing techniques and the use of time-honored herbal supplements.‘Stress response’ are many and varied-marriage, divorce, death of a loved one, new job, move, etc.-any intense emotion such as fear or anger, fatigue, physical injury, surgery, temperature extremes, noise, crowding, illness.
    There is a definite relationship between stress and illness, although the exact nature of this relationship is not yet fully understood. The more frequent and severe the life changes, the more likely we are to become ill.Combination of key traditional anti-stress herbs may help settle down your world and step up your energy levels to dynamic new heights.

  • Preparation and Evaluation Antioxidant Activity of Mixed Herbal Hair Oil Formulation

    About Authors: Akram Ahmad(1)*,Vikas Kumar(1) ,G.P.Mohanta(2) and Hasna Ali(2).
    1-Christian school of pharmacy, FHMSIAMS (SHIATS) Allahabad (UP)-211007.
    2-Department of pharmacy practice,Annamalai University,Chidambaram (T.N)-608002.

    Abstract:
    Antioxidants are helpful in increasing the blood circulation and thus help in hair growth as well as in the treatment a lot of diseases. Alopecia is a dermatological disorder with psychosocial implications on patients with hair loss. The Antioxidant property of plant and oil can be  utilise in hair fall .The objective of present study involves preparation of herbal hair oil using amla, brahmi, tridex, neem, shikakai and its evaluation for increase in hair growth activity. The oils with two different drug concentrations 5% & 10% were prepared using direct boiling, paste and cloth method. For determination of antioxidant activity DPPH method is used. Herbal hair oil formulation showing moderate antioxidant activity when compared with standard.it can be used as herbal hair oil for hair growth and other Free radical damage within cells has been linked to a range of disorders including cancer, arthritis, atherosclerosis, Alzheimer's disease, and diabetes.

  • Tissue Culture of Endangered Brahmi (Bacopa monnieri) (Linn) family Scrophulariaceae

    About Authors: - Tiwari1 Bhrigu Narayan*, Mr. Khatri1 Pankaj, Dr. Ali2 Julfikar.
    1 . Department of Pharmacognosy Gyan Vihar School of Pharmacy S.G.V. University ,Jaipur (Rajasthan)
    2. Department of Biotechnology, School of Science S.G.V .University, Jaipur (Rajasthan)

    Abstract
    Tissue culture
    (often called micropropagation) is a special type of asexual propagation where a very small piece of tissue (shoot apex, leaf section, or even an individual cell) is excised (cut-out) and placed in sterile (aseptic) culture in a test tube, petri-dish or tissue culture container containing a special culture medium.Several industries and nurseries have standardized protocols for the multiplication of ornamentals like orchids genera, carnation, roses, chrysanthemum and plantation crop like banana and cardamom..The compounds responsible for the memory enhancing effects of Bacopa monniera are triterpenoid saponins called bacosides .In the Ayurvedic Materia Medica, Bacopa has been recognized for its brain enhancement characteristics. Bacopa monnieri, a member of the Scrophulariaceae family, is a small, creeping herb with numerous branches, small oblong leaves and light purple flowers. In India and the tropics it grows naturally in wet soil, shallow water and marches. Brahmi (Bacopa monnieri) (Linn) familyScrophulariaceaeis also known as Madhyarasayana in Ayurveda as it increase mental-clarityand brain stimulating action It also possesses anti-inflammatory, analgesic, antipyretic, epilepsy, insanity, anticancer and antioxidant activities, saponins such as bacosides A, B, C and D which are the active triterpenoid principles and known as "memory chemicals".Only  a  very limited research has been  carried  out  on the plant,  under  the present study  assumes singular  significance and it is supposed to contribute a great deal to the existing literature. The present paper highlights the morphogenic response of various auxins and cytokinins on Bacopa monniera.

  • Eclipta alba (L.) A review on its phytochemical and pharmacological profile

    About Authors:
    Mithun NM*, Dr.Shashidhara S1

    Dept. of Pharmacognosy,
    Government college of pharmacy,
    Bangalore

    Abstract: Eclipta alba (L.) is small branched annual herbaceous plant with a long history of traditional medicines uses in many countries especially in tropical and subtropical regions. The herb has been known for its curative properties and has been utilized as antimytotoxic, analgesic, antibacterial, antihepatotoxic, antihaemorrhagic, antihyperglycemic, antioxidant, immunomodulatory properties and it is considered as a good rejuvenator too. Recent studies showed an antivenom property & corrosion pickling inhibitor action on mild steel in hydrochloric acid. A wide range of chemical compounds including coumestans, alkaloids, thiopenes, flavonoids, polyacetylenes, triterpenes and their glycosides have been isolated from this species. Extracts and metabolites from this plant have been known to possess pharmacological properties. This contribution provides an comprehensive review on ethnomedicinal uses, chemical composition, and the pharmacological profile as medicinal plant. Particular attention is given to antihepatotoxic, analgesic, antioxidant , antihyperglycemic, antiaggresive, wound healing properties and insecticidal effects presented in this review such that the potential use of this plant either in pharmaceutics or as an agricultural resource can be evaluated.

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