EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF FICUS RELIGIOSA ON RATS AGAINST CCL4 AND PARACETAMOL INDUCED HEPATOTOXICITY

 

Phytochemical Screening
Standard phytochemical methods were used to test for the presence of saponins, alkaloids, tannins, anthraquinones, cardiac glycosides, cyanogenetic glycosides, amino acid & protein and flavonoids.

Animal treatment in CCl4 induced Hepatotoxicity
Rats were divided into five groups of six animals in each group. Table 1 shows the dose schedule of carbon tetrachloride and test samples against CCl4 intoxication. Group 1 and 2 was treated for seven days with 2? accasia, group 3rd treated with silymarin and group 4th  treated with aqous extract and group 5th treated with methanolic extact. On seventh day 30 min after dosing group 2nd,3rd,4th,5th was treated with CCl4 1.5ml/kg (1:1 ccl4 in olive oil) orally.The animals were sacrificed after 36 h after administration of acute dose of CCl4. The blood was collected by carotid artery. Biochemical parameter aspartate aminotransferase (AST), alanine aminotransferase (ALT) were tested in Awasti Diagnostic Center, Bhopal  using Span diagnostic kits.

Table – 1Groups & Doses schedule for CCl4 (Carbon tetrechloride) induced hepatotoxicity

SL.NO

 GROUP

TREATMENT

   1

      I

Control received 2% acacia for 7 days (o.d.)

   2

     II

2% acacia for 7 days + ccl4 1.5ml/kg (1:1 ccl4 in olive oil) orally

   3

    III

Silymarin (100mg/kg/day,P.O.) 7 days +CCl4 on 7 day

   4

    IV

Aq. Extract (200mg/kg/day) fine suspension of 2% acacia + CCl4 on 7 day

   5

    V

Methanol Extract (200mg/kg/day) fine suspension of 2%  acacia + CCl4 on 7 day

Animal treatment in paracetamol  induced Hepatotoxicity
This model is used to produced experimental liver damage only, since rat are resistant to paracetamol induced hepatotoxicity. Paracetamol administered orally as a single dose of 500mg/kg in rate produce hepatotoxicity. Paracetamol is administered as a single dose of 500mg/kg. After 48hrs, they treated with the test drug for 7th days. At the end of experiment the blood was collected from carotid artery. Biochemical parameter aspartate aminotransferase (AST), alanine aminotransferase (ALT) were tested in Awasti Diagnostic Center, Bhopal  using Span diagnostic kits.

Table - 2Groups & Doses schedule for Paracetamol induced hepatotoxicity

SL.NO

GROUP

TREATMENT

1

      I

Control received 2% acacia for 5 days (o.d.)

2

     II

2% acacia for 5 days + Paracetamol (500mg/kg) for one day only

3

    III

Silymarin (100mg/kg/day,P.O.) 5 days + Paracetamol (500mg/kg)  for one day only

4

    IV

Aq. Extract (200mg/kg/day) fine suspension of 2% acacia + Paracetamol (500mg/kg)  for one day only

5

     V

Methanol Extract (200mg/kg/day) fine suspension of 2% acacia + Paracetamol (500mg/kg)  for one day only

Result & Observation
Result

Table 3 Effect of ficus religiosa on serum biochemical parameter in Paracetomol & Carbon tetrachloride induced hepatic toxicity in rat

GROUP

 DOSE

 SGOT(U/L)

 SGPT(U/L)

Group 1 (vehicle)

 1ml/kg

98.5±4.7

72.86±6.19

Group 2 (CCl4)

 1.5ml/kg

141.13±9.30

119.30±17.2

Group 3 (std.+ CCl4)

 100mg/kg

73.65±3.10**

57.22±2.81**

Group 4 (aqs.+ CCl4)

 200mg/kg

115±5.87*

98.00±8.9**

Group 5 (mtoh.+ CCl4)

 200mg/kg

103.89±5.7**

77.32±10.7**

Groups from II to V received CCl4 30 min after treatment on 7th day.

The data obtained were analyzed by one way ANOVA followed by Dunnett Multiple Comparisons Test. Each values represent the  mean ± SEM; n=6. **p< 0.01*p< 0.05,vs. Group II

Table 4. Effect of ficus religiosa on serum biochemical parameter in Paracetamol induced hepatotoxicity.

GROUP

 DOSE

 SGOT(U/L)

 SGPT(U/L)

Group 1 (vehicle)

 1ml/kg

98.5±4.7

72.86±6.19

Group 2 (pcm)

 500mg/kg

133.2±3.2

111.31±8.7

Group 3 (std.+ pcm)

 100mg/kg

69.76±2.9**

59.65±3.25**

Group 4 (aqs.+pcm)

 200mg/kg

119.15±4.39*

95.11±5.71ns

Group 5 (mtoh.+ pcm)

 200mg/kg

111.3±3.1**

79.88±4.5**

Groups from II to V received PCM 48 hrs before treatment on day.

The data obtained were analyzed by one way ANOVA followed by Dunnett Multiple Comparisons Tests. Each values represent the mean ± SEM; n=6. **p< 0.01*p< 0.05, ns p? 0.05 vs. Group II.

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